Toxoplasma gondii P30 antibody and antigen (recombinant protein)

 Download 

Diagnostic anti-Toxoplasma gondii P30 antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Swine/Porcine/Pig, Avian/Bird/Poultry, Ruminants infectious disease Toxoplasmosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

Go to Multiple species disease testing products collection >>

Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P278-Tg001-Ag01	Recombinant Toxoplasma gondii P30 protein	$3090.00
GMP-VT-P278-Tg001-Ab01	Anti-Toxoplasma gondii P30 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P278-Tg001-Ab02	Anti-Toxoplasma gondii P30 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P278-Tg001-Ab03	Anti-Toxoplasma gondii P30 human monoclonal antibody (mAb)	$3090.00
GMP-VT-P278-Tg001-Ab04	Anti-Toxoplasma gondii P30 human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P278-Tg001-Ag01
Product Name	Recombinant Toxoplasma gondii P30 protein
Pathogen	Toxoplasma gondii
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Toxoplasma gondii P30 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Toxoplasma gondii level test of animal Cat/Feline, Dog/Canine, Swine/Porcine/Pig, Avian/Bird/Poultry, Ruminants infectious disease with Toxoplasmosis.
Tag	His
Product description	Recombinant Toxoplasma gondii P30 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P278-Tg001-Ab01,GMP-VT-P278-Tg001-Ab02
Pathogen	Toxoplasma gondii
Product Name	Anti-Toxoplasma gondii P30 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Toxoplasma gondii P30 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Toxoplasma gondii antibodies in Toxoplasma gondii level test of animal Cat/Feline, Dog/Canine, Swine/Porcine/Pig, Avian/Bird/Poultry, Ruminants infectious disease with Toxoplasmosis.
Product description	Anti-Toxoplasma gondii P30 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Toxoplasma gondii antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P278-Tg001-Ab03,GMP-VT-P278-Tg001-Ab04
Pathogen	Toxoplasma gondii
Product Name	Anti-Toxoplasma gondii P30 human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Toxoplasma gondii P30 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Toxoplasma gondii antibodies in Toxoplasma gondii level test of animal Cat/Feline, Dog/Canine, Swine/Porcine/Pig, Avian/Bird/Poultry, Ruminants infectious disease with Toxoplasmosis.
Product description	Anti-Toxoplasma gondii P30 mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Pathogen Information

Toxoplasma gondii (T. gondii) is a remarkable and extensively studied parasitic protozoan that belongs to the phylum Apicomplexa. This pathogen possesses a fascinating biology and is of significant medical and scientific interest due to its complex life cycle and potential impact on human health.

Pathogen Name and Description:

Toxoplasma gondii, often referred to as T. gondii, is an obligate intracellular parasite with a broad host range. It is classified within the phylum Apicomplexa, a diverse group of unicellular eukaryotic microorganisms known for their apical complex, which plays a crucial role in host cell invasion.

Pathogen Classification:

T. gondii falls under the taxonomical classification of:

1.Kingdom: Protista

2.Phylum: Apicomplexa

3.Class: Conoidasida

4.Order: Eucoccidiorida

5.Family: Sarcocystidae

6.Genus: Toxoplasma

7.Species: gondii

The apicomplexans are characterized by their unique organelles, such as the apicoplast and micronemes, which are involved in host cell attachment and invasion.

Pathogen Structure (Key Genes and Proteins):

Toxoplasma gondii possesses a complex structure, with several key genes and proteins that are instrumental in its life cycle. Some of the notable ones include:

8.SAG1 (Surface Antigen 1): SAG1 is encoded by the SAG1 gene and is a critical protein for host cell recognition and attachment during the early stages of the parasite's invasion.

9.ROP2 (Rhoptry Protein 2): ROP2 is part of the rhoptry proteins, which are secreted by T. gondii upon entering the host cell. ROP2 modulates host cell processes to favor the parasite's survival and replication.

10.GRA1 (Dense Granule Protein 1): The GRA1 gene encodes a protein found in the dense granules of the parasite. These granules are secreted during the invasion process and contribute to the formation of the parasitophorous vacuole, a specialized compartment where the parasite resides and replicates.

The interplay of these genes and proteins is essential for the successful infection of host cells by T. gondii.

Hosts and Associated Diseases:

One of the striking features of T. gondii is its ability to infect a vast range of warm-blooded hosts, including mammals and birds. In humans, this parasite can cause a disease known as toxoplasmosis. Toxoplasmosis can manifest with a wide spectrum of clinical presentations, depending on the immune status of the host.

11.Immunocompetent Individuals: In healthy individuals, the infection is often asymptomatic or results in mild, flu-like symptoms. After the initial acute phase, the parasite forms dormant cysts in various tissues, most notably the brain and muscles, where it can persist for the host's lifetime.

12.Immunocompromised Individuals: Immunocompromised individuals, such as those with HIV/AIDS or undergoing immunosuppressive therapy, are at greater risk of developing severe toxoplasmosis. This can lead to encephalitis and other life-threatening complications.

13.Congenital Toxoplasmosis: Pregnant women who acquire a primary infection during pregnancy can transmit the parasite to their developing fetus, potentially leading to congenital birth defects.

Diagnostic Methods and Target Genes/Proteins:

The precise diagnosis of T. gondii infection is crucial for effective clinical management. Various diagnostic methods are employed, each targeting specific genes and proteins associated with the parasite:

14.Serological Tests: These assays detect specific antibodies, such as IgM and IgG, against T. gondii in blood samples. Antibody production is a marker of previous or current infection.

15.PCR (Polymerase Chain Reaction): PCR-based tests directly identify T. gondii DNA in clinical specimens. Targeted genes for PCR detection may include the B1 gene, which is highly conserved among T. gondii strains, GRA6, or the 18S rRNA gene.

16.ELISA (Enzyme-Linked Immunosorbent Assay): ELISA is used to detect T. gondii-specific antibodies. Common protein targets include SAG1 and SAG2, which are surface antigens recognized by the host immune system during infection.

17.Immunofluorescence Assay (IFA): IFA is a technique that identifies antibodies in clinical specimens using fluorescence-labeled antibodies that bind to T. gondii-specific antigens, facilitating precise and specific detection.

In conclusion, Toxoplasma gondii is a complex and adaptable parasite with a remarkable ability to infect a wide range of hosts. Its biology, including key genes and proteins, is a subject of intense research, aiming to better understand the mechanisms of host cell invasion, immune evasion, and persistence. Additionally, the potential health impact of T. gondii in humans underscores the importance of accurate diagnostic methods and ongoing research to develop strategies for prevention and treatment. This detailed overview provides a comprehensive understanding of the parasite and its significance in the field of parasitology and medical science.

About GDU