Escherichia coli antibody and antigen (recombinant protein)

Diagnostic anti-Escherichia coli antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Deer, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease cholecystitis, bacteremia, cholangitis, urinary tract infection (UTI), and traveler's diarrhea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P282-Tg002-Ag01 Recombinant Escherichia coli protein $3090.00
GMP-VT-P282-Tg002-Ab01 Anti-Escherichia coli mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P282-Tg002-Ab02 Anti-Escherichia coli mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P282-Tg002-Ab03 Anti-Escherichia coli human monoclonal antibody (mAb) $3090.00
GMP-VT-P282-Tg002-Ab04 Anti-Escherichia coli human monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P282-Tg002-Ag01
Product Name Recombinant Escherichia coli protein
Pathogen Escherichia coli
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Escherichia coli antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Escherichia coli level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Deer, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with cholecystitis, bacteremia, cholangitis, urinary tract infection (UTI), and traveler's diarrhea.
Tag His
Product description Recombinant Escherichia coli proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P282-Tg002-Ab01,GMP-VT-P282-Tg002-Ab02
Pathogen Escherichia coli
Product Name Anti-Escherichia coli mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Escherichia coli antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli antibodies in Escherichia coli level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Deer, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with cholecystitis, bacteremia, cholangitis, urinary tract infection (UTI), and traveler's diarrhea.
Product description Anti-Escherichia coli mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli antibodies.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P282-Tg002-Ab03,GMP-VT-P282-Tg002-Ab04
Pathogen Escherichia coli
Product Name Anti-Escherichia coli human monoclonal antibody (mAb)
Expression platform CHO
Isotypes Human lgG1
Bioactivity validation Recombinant Escherichia coli antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli antibodies in Escherichia coli level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Deer, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with cholecystitis, bacteremia, cholangitis, urinary tract infection (UTI), and traveler's diarrhea.
Product description Anti-Escherichia coli mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


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    Pathogen Information


    Escherichia coli, commonly referred to as E. coli, is a Gram-negative, rod-shaped bacterium that is a member of the Enterobacteriaceae family. It is a prokaryotic organism that is commonly found in the intestines of humans and other animals.

    E. coli has a simple cell structure consisting of a cytoplasm, cell membrane, and cell wall. Its genome comprises a single circular chromosome that contains approximately 4.6 million base pairs. The genome size of E. coli is relatively small compared to other bacteria, which makes it an ideal organism for studying molecular biology and genetic manipulation.

    The genome of E. coli contains several thousand genes, with its main genetic components being the DNA gyrase gene, which regulates DNA replication and maintains the bacteria's shape, and RNA polymerase, which is involved in transcription and gene expression. E. coli also contains plasmids, which are small, circular pieces of DNA that can replicate independently of the chromosomal DNA. Plasmids often contain genes that provide the bacteria with advantages such as antibiotic resistance or the ability to produce virulence factors.

    E. coli primarily infects humans and other animals through the ingestion of contaminated food or water. It adheres to the intestinal epithelium and produces toxins that damage the cells, leading to inflammation and diarrhea. Some strains of E. coli are highly virulent, such as E. coli O157:H7, which can cause severe illness and even death, most notably through Hemolytic Uremic Syndrome (HUS).

    E. coli is responsible for several diseases in humans, including urinary tract infections (UTIs), meningitis, pneumonia, and sepsis. UTIs are one of the most common bacterial infections in humans, with E. coli accounting for up to 80% of cases. Meningitis, or inflammation of the membranes surrounding the brain and spinal cord, is a severe infection that can be caused by various bacteria, including E. coli. Pneumonia is an infection of the lungs that can be caused by many types of bacteria, including E. coli. Sepsis is a life-threatening condition in which the body's immune response to an infection damages its own tissues and organs.

    Various diagnostic methods are available for detecting E. coli infections, including culture-based methods and nucleic acid-based methods. Culture-based methods involve growing the bacteria in the laboratory and identifying it based on its characteristics. These methods include the use of selective media, such as MacConkey agar, that only allow Gram-negative bacteria like E. coli to grow. Nucleic acid-based methods utilize specific genes or proteins related to E. coli, such as the rfbE gene for O157:H7 serotype detection and stx1/stx2 genes for detecting Shiga toxin-producing E. coli (STEC) strains. Polymerase Chain Reaction (PCR) is an example of a widely used nucleic acid-based detection method.

    In conclusion, Escherichia coli is a prokaryotic bacterium that can cause a range of illnesses in humans and other animals. It has a relatively small genome size, which makes it an ideal organism for studying molecular biology and genetic manipulation. Various diagnostic methods are available for detecting E. coli infections, including culture-based methods and nucleic acid-based methods targeting specific genes or proteins.



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