Escherichia coli F4 fimbrial adhesin FaeG antibody and antigen (recombinant protein)

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Diagnostic anti-Escherichia coli F4 fimbrial adhesin FaeG antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease post-weaning diarrhea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P283-Tg001-Ag01	Recombinant Escherichia coli F4 fimbrial adhesin FaeG protein	$3090.00
GMP-VT-P283-Tg001-Ab01	Anti-Escherichia coli F4 fimbrial adhesin FaeG mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P283-Tg001-Ab02	Anti-Escherichia coli F4 fimbrial adhesin FaeG mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P283-Tg001-Ab03	Anti-Escherichia coli F4 fimbrial adhesin FaeG human monoclonal antibody (mAb)	$3090.00
GMP-VT-P283-Tg001-Ab04	Anti-Escherichia coli F4 fimbrial adhesin FaeG human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P283-Tg001-Ag01
Product Name	Recombinant Escherichia coli F4 fimbrial adhesin FaeG protein
Pathogen	Escherichia coli F4
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Escherichia coli F4 fimbrial adhesin FaeG antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Escherichia coli F4 level test of animal Swine/Porcine/Pig infectious disease with post-weaning diarrhea.
Tag	His
Product description	Recombinant Escherichia coli F4 fimbrial adhesin FaeG proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P283-Tg001-Ab01,GMP-VT-P283-Tg001-Ab02
Pathogen	Escherichia coli F4
Product Name	Anti-Escherichia coli F4 fimbrial adhesin FaeG mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Escherichia coli F4 fimbrial adhesin FaeG antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli F4 antibodies in Escherichia coli F4 level test of animal Swine/Porcine/Pig infectious disease with post-weaning diarrhea.
Product description	Anti-Escherichia coli F4 fimbrial adhesin FaeG mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli F4 antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P283-Tg001-Ab03,GMP-VT-P283-Tg001-Ab04
Pathogen	Escherichia coli F4
Product Name	Anti-Escherichia coli F4 fimbrial adhesin FaeG human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Escherichia coli F4 fimbrial adhesin FaeG antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli F4 antibodies in Escherichia coli F4 level test of animal Swine/Porcine/Pig infectious disease with post-weaning diarrhea.
Product description	Anti-Escherichia coli F4 fimbrial adhesin FaeG mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Escherichia coli F4: A Comprehensive Pathogen Profile

Escherichia coli F4, often referred to as E. coli F4 or Enterotoxigenic Escherichia coli F4 (ETEC F4), is a notable pathogenic bacterial strain, warranting an in-depth exploration. This comprehensive pathogen profile provides a detailed overview of E. coli F4, delving into its classification, structural attributes, hosts infected, diseases caused, and diagnostic methods employed for its identification.

Pathogen Classification:

E. coli F4, as the name suggests, is a strain of Escherichia coli, a bacterial species that belongs to the Escherichia genus within the family Enterobacteriaceae. Taxonomically, it is classified under the domain Bacteria, specifically in the phylum Proteobacteria. Within Proteobacteria, E. coli F4 is placed in the class Gammaproteobacteria and order Enterobacterales. This classification provides insights into the pathogen's phylogenetic relationships and underscores its prokaryotic nature.

Pathogen Description:

Escherichia coli F4 falls into the category of enterotoxigenic E. coli (ETEC) strains. ETEC strains are renowned for their ability to produce enterotoxins, which are the primary culprits behind the induction of diarrhea in their hosts. E. coli F4 is particularly noteworthy for its role in causing diseases in specific host species.

Pathogen Structure:

The structural composition of E. coli F4 is characterized by a complex interplay of genes and proteins. These genetic elements include a repertoire of genes responsible for the production of virulence factors. Among the notable virulence factors are enterotoxins, which play a central role in the pathogenesis of E. coli F4-induced diseases. Additionally, adhesion factors are crucial components of the pathogen's structure, facilitating its ability to adhere to host cells and initiate infection. The precise genetic and protein components involved can vary among E. coli strains and are a subject of ongoing research.

Hosts Infected and Diseases Caused:

Escherichia coli F4 primarily targets domestic animals, with piglets being especially susceptible to its pathogenic effects. Infection by E. coli F4 in piglets can result in the development of specific diseases, including neonatal diarrhea and post-weaning diarrhea. Collectively, these diseases fall under the umbrella of enterotoxigenic colibacillosis in pigs. The consequences of such infections can extend to economic losses in the livestock industry due to reduced growth rates, increased mortality, and the cost of treatment.

Diagnostic Methods:

Accurate and timely diagnosis of E. coli F4 infection is pivotal for effective disease management and control. Several diagnostic methods are employed, catering to both traditional microbiological techniques and advanced molecular approaches:

1.Microbiological Isolation and Identification: This involves the isolation of E. coli F4 from clinical samples, typically sourced from feces or intestinal tissues. The bacterium's characteristic growth and biochemical reactions are used for identification.

2.Serological Testing: Serological assays are utilized to detect antibodies generated by the host against E. coli F4. The presence of specific antibodies indicates exposure and immune response.

3.Polymerase Chain Reaction (PCR): Molecular methods, particularly PCR, are invaluable for the detection of E. coli F4. Targeting specific genes associated with the pathogen, such as "lt" (heat-labile toxin gene) and "sta" (heat-stable toxin gene), PCR offers rapid and highly specific confirmation of the pathogen's presence.

4.DNA Sequencing: DNA sequencing techniques are employed to further validate the presence of distinct E. coli F4 genes. This provides a level of specificity and accuracy that is essential in identifying the pathogen.

5.Enzyme-Linked Immunosorbent Assay (ELISA): ELISA is a sensitive and specific method used to detect E. coli F4 antigens. It plays a crucial role in identifying the pathogen in clinical samples.

These diagnostic methods collectively form a robust toolkit for the identification and confirmation of E. coli F4 in affected animals. Timely and accurate diagnosis is pivotal in implementing appropriate control measures and minimizing the impact of E. coli F4-induced diseases on livestock populations.

In conclusion, Escherichia coli F4 is a pathogenic bacterial strain with a particular affinity for piglets, causing diseases that can lead to significant economic losses in the livestock industry. Its classification within the Escherichia genus and its prokaryotic nature make it a distinct member of the enterotoxigenic E. coli family. Understanding its structure, host specificity, and diagnostic methods is essential for managing and mitigating the impact of E. coli F4 infections in domestic animals.

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