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Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre)

Cat No. Pre-made Cre Viral vector Protomer Promoter Tissue Specificity Promoter Tissue
Specificity characteristics
GMV-AdCre-01Ad-CMV-CreAdenovirusCMV Total Total
GMV-AdCre-02Ad-CMV-Cre-GFPCMV Total Total
GMV-AAV9Cre-01AAV9-CMV-CreAAV

GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option:
· AAV1-Cre
· AAV2-Cre
· AAV2 variant (Y444F)-Cre
· AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre
· AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre
· AAV2 variant(AAV2.7m8)-Cre
· AAV5-Cre
· AAV6-Cre
· AAV8-Cre
· AAV8-1m-Cre
· AAV8-2m-Cre
· AAV8 variant (Y733F, Y447F, Y275)-Cre
· AAV9-Cre
· AAV-Rh.10-Cre
· AAV-DJ-Cre
· AAV-DJ/8-Cre
· AAV-Retro (Retrograde)-Cre
· AAV9-PHP.B-Cre
· AAV9-PHP.A-Cre
· AAV9-PHP.eB-Cre
· AAV9-PHP.S-Cre
CMV Total Total
GMV-AAV9Cre-02AAV9-CMV-Cre-ZsGreen CMV Total Total
GMV-AAV9Cre-03AAV9-CAG-Cre CAG Total Total
GMV-AAV9Cre-04AAV9-CAG-Cre-ZsGreen CAG Total Total
GMV-AAV9Cre-05AAV9-Syn-Cre-EGFP synapsin (SYN) NeuroNeuron-specific promoter
GMV-AAV9Cre-06AAV9-Syn-Cre synapsin (SYN) NeuroNeuron-specific promoter
GMV-AAV9Cre-07AAV9-CaMKII-Cre-EGFP CaMKIINeuroForebrain glutamate neuron-specific promoter
GMV-AAV9Cre-08AAV9-CaMKII-Cre CaMKIINeuroForebrain glutamate neuron-specific promoter
GMV-AAV9Cre-09AAV9-GFAP-Cre-EGFP GFAPNeuroAstrocyte specific promoter
GMV-AAV9Cre-10AAV9-GFAP-Cre GFAPNeuroAstrocyte specific promoter
GMV-AAV9Cre-11AAV9-cTNT-Cre cTnTheartCardiomyocyte specific promoter
GMV-AAV9Cre-12AAV9-cTNT-Cre-EGFP cTnTheartCardiomyocyte specific promoter
GMV-AAV9Cre-13AAV9-TBG-Cre TBGliverHepatic specific promoter
GMV-AAV9Cre-14AAV9-TBG-Cre-ZsGreen TBGliverHepatic specific promoter
GMV-AAV9Cre-15AAV9-TIE-Cre TIEendothelialEndothelial-specific promoter
GMV-AAV9Cre-16AAV9-cd68-Cre cd68NeuroMicroglia-specific promoter
GMV-AAV9Cre-17AAV9-c-fos-Cre c-fosNeuroExcitatory neuron promoter
GMV-AAV9Cre-18AAV9-mecp2-Cre mecp2NeuroShorter neuron-specific promoter
GMV-AAV9Cre-19AAV9-MHCK7-Cre MHCK7muscleMuscle specific promoter
GMV-AAV9Cre-20AAV9-Rpe65-Cre Rpe65retinaRetinal specific promoter
GMV-AAV9Cre-21AAV9-sm22a-Cre sm22amuscleSmooth muscle specific promoter
GMV-AAV9Cre-22AAV9-Spc5-12-Cre Spc5-12muscleMyocyte specific promoter
GMV-AAV9Cre-23AAV9-Ta1-Cre TalNeuroEarly neuron-specific promoter
GMV-LvCre-01Lv-CMV-CreLentivirusCMV Total Total
GMV-LvCre-02Lv-CMV-Cre-zsgreenCMV Total Total



Abstract

Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI). To date, numerous systems, derived from the basic Cre-loxP system, have been developed to precisely control gene expression at desired time or cells. Here, we briefly introduce Cre-loxP system, and give a summary about its applications, especially viral vectors-mediated Cre expression (such as AAV-Cre or Ad-Cre) for in vitro or in vivo studies.

AAV-DIO in neuroscience

In the research field of neuroscience, AAV-DIO shows outstanding spatiotemporal fidelity and is perfectly suitable for examining and manipulating neuronal function in vivo, such as studies in neural circuits and networks, behavior and so on. Optogenetics integrates optics with genetic engineering to measure or manipulate the biomolecular processes, especially in neuroscience [108]. By delivering optical signals with specific wavelength to precisely detect, measure, or control biological processes in living tissues, optogenetics opens up new landscapes for the study of biology, both in health and disease [109]. With the combination of AAV-DIO and optogenetics, researchers control and monitor the activities of individual neurons, and precisely measure the manipulation effects in real-time. For example, in Fig. 8, with AAV-DIO system, the neuron X specifically express opsin (such ChR2) and is labeled by the reporter gene (such as mCherry), but remains silenced. When exposed to light in correct wavelength, neuron X will be activated (Fig. 8) [110].

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Figure 8. Principle of optogenetics tool in combination with AAV-DIO. With no light in specific wavelength matched to opsin, such as ChR2, ChR2 gene and the reporter gene is specifically expressed in neurons (location in red). Once exposed to light with the matched wavelength, ChR2 cation channels will be open and Na+ flow into neural cells. Thus, the neuron will be activated.


Genemedi possesses a complete library of optogenetics components into serotypes of AAV vector, which is listed in table 5. You can find more information on this website: https://www.genemedi.net/i/aav-optogenetics.

Table 5. List of optogenetics tools in AAV
AAV Plasmids Promoter and Expression Characteristics Fluorescent Protein Label Cre dependent Optogenetics effect
pAAV-CAG-DIO-eNpHR3.0-EYFPCAG general and strong expressionEYFPyesInhibition
pAAV-CAG -DIO-hChR2(H134R)-mCherry mCherryyesactivation
pAAV-CAG-DIO-ArchT-EYFP EYFPyesinhibition
pAAV-CAG -DIO-C1V1 (t/t)-TS-mCherry mCherryyesactivation
pAAV-CAG-DIO-Arch3.0-EYFP EYFPyesinhibition
pAAV- CAG -DIO-hCHETA-EYFP EYFPyesactivation
pAAV-CMV-DIO-eNpHR3.0-EYFPCMV(general and strong expression)EYFPyesinhibition
pAAV-CMV-DIO-hChR2(H134R)-mCherry mCherryyesactivation
pAAV-CMV-DIO-ArchT-EYFP EYFPyesinhibition
pAAV-CMV-DIO-C1V1 (t/t)-TS-mCherry mCherryyesactivation
pAAV-CMV-DIO-Arch3.0-EYFP EYFPyesinhibition
pAAV-CMV-DIO-hCHETA-EYFP EYFPyesactivation
pAAV-hSyn-eNpHR3.0-EYFPhSyn neuron specific expressionEYFPNoinhibition
pAAV-hSyn-hChR2(H134R)-mCherry mCherryNoactivation
pAAV-hSyn-ArchT-EYFP EYFPNoinhibition
pAAV-hSyn-C1V1-(t/t)-TS-mCherry mCherryNoactivation
pAAV-hSyn-Arch3.0-EYFP EYFPNoinhibition
pAAV-hSyn-DIO-hCHETA-EYFP EYFPyesactivation
pAAV-GFAP-eNpHR3.0-EYFPGFAP astrocyte specific expressionEYFPNoinhibition
pAAV-GFAP-hChR2(H134R)-mCherry mCherryNoactivation
pAAV-GFAP-ArchT-EYFP EYFPNoinhibition
pAAV-GFAP-C1V1 (t/t)-TS-mCherry mCherryNoactivation
pAAV-GFAP-Arch3.0-EYFP EYFPNoinhibition
pAAV-GFAP-hCHETA-EYFP EYFPNoactivation
pAAV-CaMKII-eNpHR3.0-EYFPCaMKII neuron specific expressionEYFPNoinhibition
pAAV-CaMKII-hChR2(H134R)-mCherry mCherryNoactivation
pAAV-CaMKII-ArchT-EYFP EYFPNoinhibition
pAAV-CaMKII-C1V1 (t/t)-TS-mCherry mCherryNoactivation
pAAV-CaMKII-Arch3.0-EYFP EYFPNoinhibition
pAAV-CaMKII-hCHETA-EYFP EYFPNoactivation


Summary

The Cre-loxP system, especially inducible tissue-specific knockout and viral vector-mediated Cre expression, has been highly utilized in genetics and cell biology research. Nevertheless, it seems that Cre-loxP system will continue to be prevalent in current and future research studies. GeneMedi is proficient in viral vector development and offers kinds of viral vector-based Cre tools, we can provide the best services and products if required.

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