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1. What is the recommended dilution range for Femto Signal Western ECL Substrate?

Primary antibody recommended dilution range is 1 to 0.2 µg/mL primary antibody (1:5,000 to 1:100,000 dilution from 1µg/mL stock). Secondary antibody recommended dilution range is 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from µg/mL stock).


2. What is the sensitivity of Femto Signal Western ECL Substrate?

Femto Signal Western ECL Substrate enables detection of low-femtogram amounts of target protein on nitrocellulose or PVDF membrane.