Human NME1/AWD/GAAD ORF/cDNA clone-Lentivirus plasmid (NM_000269)

Cat. No.: pGMLP000505
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human NME1/AWD/GAAD Lentiviral expression plasmid for NME1 lentivirus packaging, NME1 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

Our GM-Lentiviral vector is seamlessly integrated with the GM lentivirus packaging system. Discover more about the GM lentivirus packaging system.

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Product Description

Catalog ID pGMLP000505
Gene Name NME1
Accession Number NM_000269
Gene ID 4830
Species Human
Product Type Lentivirus plasmid (overexpression)
Insert Length 459 bp
Gene Alias AWD,GAAD,NB,NBS,NDKA,NDPK-A,NDPKA,NM23,NM23-H1
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGCCAACTGTGAGCGTACCTTCATTGCGATCAAACCAGATGGGGTCCAGCGGGGTCTTGTGGGAGAGATTATCAAGCGTTTTGAGCAGAAAGGATTCCGCCTTGTTGGTCTGAAATTCATGCAAGCTTCCGAAGATCTTCTCAAGGAACACTACGTTGACCTGAAGGACCGTCCATTCTTTGCCGGCCTGGTGAAATACATGCACTCAGGGCCGGTAGTTGCCATGGTCTGGGAGGGGCTGAATGTGGTGAAGACGGGCCGAGTCATGCTCGGGGAGACCAACCCTGCAGACTCCAAGCCTGGGACCATCCGTGGAGACTTCTGCATACAAGTTGGCAGGAACATTATACATGGCAGTGATTCTGTGGAGAGTGCAGAGAAGGAGATCGGCTTGTGGTTTCACCCTGAGGAACTGGTAGATTACACGAGCTGTGCTCAGAACTGGATCTATGAATGA
ORF Protein Sequence MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-T42533-Ab Anti-NDKA/ NM23-H1/ NME1 functional antibody
    Target Antigen GM-Tg-g-T42533-Ag NM23-H1/NME1 protein
    ORF Viral Vector pGMLP000505 Human NME1 Lentivirus plasmid
    ORF Viral Vector pGMAP000091 Human NME1 Adenovirus plasmid
    ORF Viral Vector vGMLP000505 Human NME1 Lentivirus particle
    ORF Viral Vector vGMAP000091 Human NME1 Adenovirus particle


    Target information

    Target ID GM-T42533
    Target Name NM23-H1
    Gene ID 4830, 100533956, 101088196
    Gene Symbol and Synonyms AWD,GAAD,NB,NBS,NDKA,NDPK-A,NDPKA,NM23,NM23-H1,NME1
    Uniprot Accession P15531
    Uniprot Entry Name NDKA_HUMAN
    Protein Sub-location Secreted Protein/Potential Cytokines
    Category Therapeutics Target
    Disease Cancer
    Gene Ensembl ENSG00000239672
    Target Classification Tumor-associated antigen (TAA)

    This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]



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