Human MRPS27/MRP-S27/S27mt ORF/cDNA clone-Lentivirus plasmid (NM_015084)

Cat. No.: pGMLP002555
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human MRPS27/MRP-S27/S27mt Lentiviral expression plasmid for MRPS27 lentivirus packaging, MRPS27 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

Our GM-Lentiviral vector is seamlessly integrated with the GM lentivirus packaging system. Discover more about the GM lentivirus packaging system.

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Product Description

Catalog ID pGMLP002555
Gene Name MRPS27
Accession Number NM_015084
Gene ID 23107
Species Human
Product Type Lentivirus plasmid (overexpression)
Insert Length 1245 bp
Gene Alias MRP-S27,S27mt
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGCTGCCTCCATAGTGCGGCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCTTCCTCAGCTCTCTCCTGCAGGTAAAAGATACCTGCTTTCTTCAGCCTATGTAGACAGCCACAAATGGGAAGCAAGAGAAAAAGAACATTACTGTCTTGCTGATCTTGCATCTTTAATGGATAAAACATTTGAGAGAAAGTTGCCTGTTAGTTCTTTAACAATATCACGGCTTATAGACAACATTTCCTCTCGGGAAGAGATAGATCATGCAGAGTATTACCTTTACAAGTTTCGACACAGCCCCAACTGCTGGTACCTGAGAAACTGGACTATCCACACCTGGATTAGGCAGTGTCTAAAATATGATGCACAAGACAAAGCCCTATATACCCTTGTAAATAAGGTTCAATATGGAATTTTTCCAGATAACTTTACATTCAATTTACTGATGGATTCTTTCATAAAGAAAGAAAATTACAAAGATGCTTTATCTGTGGTTTTTGAGGTCATGATGCAAGAAGCCTTTGAAGTGCCTTCCACCCAACTTCTCTCCCTCTATGTTTTATTTCATTGCCTGGCAAAGAAGACAGACTTCAGTTGGGAAGAGGAGAGGAACTTTGGTGCATCCCTTTTGCTTCCAGGCCTAAAACAAAAGAACTCAGTGGGTTTCAGTTCCCAGTTGTATGGCTATGCACTTCTTGGGAAGGTGGAGTTGCAGCAAGGGCTACGGGCTGTGTACCACAACATGCCTCTGATATGGAAACCAGGCTACCTTGACAGAGCCCTTCAAGTGATGGAGAAAGTGGCTGCCTCCCCAGAAGACATAAAGCTGTGTAGAGAAGCGCTCGATGTGCTGGGTGCAGTGCTGAAGGCTCTGACTTCAGCTGATGGGGCTTCAGAGGAGCAGTCCCAAAATGATGAAGACAACCAGGGGTCAGAAAAACTGGTGGAGCAGTTAGACATCGAGGAAACAGAGCAGTCCAAGCTTCCTCAATACCTGGAACGATTTAAGGCCTTACATTCTAAGCTTCAAGCTCTGGGCAAAATTGAGTCAGAAGGTCTTTTAAGTCTGACCACCCAGCTTGTCAAGGAAAAACTCTCCACCTGTGAAGCAGAGGACATCGCCACCTATGAGCAGAATCTGCAGCAGTGGCATCTAGACCTTGTACAGTTGATCCAGAGAGAACAGCAACAGAGGGAGCAAGCGAAGCAGGAGTACCAGGCTCAGAAAGCAGCAAAGGCATCTGCCTAA
ORF Protein Sequence MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-IP2608-Ab Anti-MRPS27 monoclonal antibody
    Target Antigen GM-Tg-g-IP2608-Ag MRPS27 protein
    ORF Viral Vector pGMLP002555 Human MRPS27 Lentivirus plasmid
    ORF Viral Vector vGMLP002555 Human MRPS27 Lentivirus particle


    Target information

    Target ID GM-IP2608
    Target Name MRPS27
    Gene ID 23107, 218506, 701585, 361883, 101091995, 610387, 514740, 100073298
    Gene Symbol and Synonyms 2610028H14Rik,MRP-S27,MRPS27,S27mt
    Uniprot Accession Q92552
    Uniprot Entry Name RT27_HUMAN
    Protein Sub-location Introcelluar Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000113048
    Target Classification Not Available

    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]



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