Human RNF128/GRAIL ORF/cDNA clone-Lentivirus plasmid (NM_194463)

Cat. No.: pGMLP004137
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human RNF128/GRAIL Lentiviral expression plasmid for RNF128 lentivirus packaging, RNF128 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

Our GM-Lentiviral vector is seamlessly integrated with the GM lentivirus packaging system. Discover more about the GM lentivirus packaging system.

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Product Description

Catalog ID pGMLP004137
Gene Name RNF128
Accession Number NM_194463
Gene ID 79589
Species Human
Product Type Lentivirus plasmid (overexpression)
Insert Length 1287 bp
Gene Alias GRAIL
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGGGCCGCCGCCTGGGGCCGGGGTCTCCTGCCGCGGTGGCTGCGGCTTTTCCAGATTGCTGGCATGGTGCTTCCTGCTGGCCCTGAGTCCGCAGGCACCCGGTTCCCGGGGGGCTGAAGCAGTGTGGACCGCGTACCTCAACGTGTCCTGGCGGGTTCCGCACACGGGAGTGAACCGTACGGTGTGGGAGCTGAGCGAGGAGGGCGTGTACGGCCAGGACTCGCCGCTGGAGCCTGTGGCTGGGGTCCTGGTACCGCCCGACGGGCCCGGGGCGCTTAACGCCTGTAACCCGCACACGAATTTCACGGTGCCCACGGTTTGGGGAAGCACCGTGCAAGTCTCTTGGTTGGCCCTCATCCAACGCGGCGGGGGCTGCACCTTCGCAGACAAGATCCATCTGGCTTATGAGAGAGGGGCGTCTGGAGCCGTCATCTTTAACTTCCCCGGGACCCGCAATGAGGTCATCCCCATGTCTCACCCGGGTGCAGTAGACATTGTTGCAATCATGATCGGCAATCTGAAAGGCACAAAAATTCTGCAATCTATTCAAAGAGGCATACAAGTGACAATGGTCATAGAAGTAGGGAAAAAACATGGCCCTTGGGTGAATCACTATTCAATTTTTTTCGTTTCTGTGTCCTTTTTTATTATTACGGCGGCAACTGTGGGCTATTTTATCTTTTATTCTGCTCGAAGGCTACGGAATGCAAGAGCTCAAAGCAGGAAGCAGAGGCAATTAAAGGCAGATGCTAAAAAAGCTATTGGAAGGCTTCAACTACGCACACTGAAACAAGGAGACAAGGAAATTGGCCCTGATGGAGATAGTTGTGCTGTGTGCATTGAATTGTATAAACCAAATGATTTGGTACGCATCTTAACGTGCAACCATATTTTCCATAAGACATGTGTTGACCCATGGCTGTTAGAACACAGGACTTGCCCCATGTGCAAATGTGACATACTCAAAGCTTTGGGAATTGAGGTGGATGTTGAAGATGGATCAGTGTCTTTACAAGTCCCTGTATCCAATGAAATATCTAATAGTGCCTCCTCCCATGAAGAGGATAATCGCAGCGAGACCGCATCATCTGGATATGCTTCAGTACAGGGAACAGATGAACCGCCTCTGGAGGAACACGTGCAGTCAACAAATGAAAGTCTACAGCTGGTAAACCATGAAGCAAATTCTGTGGCAGTGGATGTTATTCCTCATGTTGACAACCCAACCTTTGAAGAAGACGAAACTCCTAATCAAGAGACTGCTGTTCGAGAAATTAAATCTTAA
ORF Protein Sequence MGPPPGAGVSCRGGCGFSRLLAWCFLLALSPQAPGSRGAEAVWTAYLNVSWRVPHTGVNRTVWELSEEGVYGQDSPLEPVAGVLVPPDGPGALNACNPHTNFTVPTVWGSTVQVSWLALIQRGGGCTFADKIHLAYERGASGAVIFNFPGTRNEVIPMSHPGAVDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFIITAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGRLQLRTLKQGDKEIGPDGDSCAVCIELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-IP1504-Ab Anti-RNF128 monoclonal antibody
    Target Antigen GM-Tg-g-IP1504-Ag RNF128 protein
    ORF Viral Vector pGMLP004137 Human RNF128 Lentivirus plasmid
    ORF Viral Vector vGMLP004137 Human RNF128 Lentivirus particle


    Target information

    Target ID GM-IP1504
    Target Name RNF128
    Gene ID 79589, 66889, 700890, 315911, 101080265, 492051, 535869, 100054821
    Gene Symbol and Synonyms 1300002C13Rik,GRAIL,Greul1,RGD1566282,RNF128
    Uniprot Accession Q8TEB7
    Uniprot Entry Name RN128_HUMAN
    Protein Sub-location Introcelluar Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000133135
    Target Classification Not Available

    The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]



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