Human RPTOR/KOG1/Mip1 ORF/cDNA clone-Mammalian (Non-Viral Vector) plasmid (NM_020761.2)

Cat. No.: pGMPC000108
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human RPTOR/KOG1/Mip1 Non-Viral expression plasmid (overexpression vector) for mouse RPTOR overexpression in unique cell transient transfection and stable cell line development.

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Product Description

Catalog ID pGMPC000108
Gene Name RPTOR
Accession Number NM_020761.2
Gene ID 57521
Species Human
Product Type Mammalian (Non-Viral Vector) plasmid (overexpression)
Insert Length 4008 bp
Gene Alias KOG1,Mip1
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag Null
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGAGTCCGAAATGCTGCAATCGCCTCTTCTGGGCCTGGGGGAGGAAGATGAGGCTGATCTTACAGACTGGAACCTACCTTTGGCTTTTATGAAAAAGAGGCACTGTGAGAAAATTGAAGGCTCCAAATCCTTAGCTCAGAGCTGGAGGATGAAGGATCGGATGAAGACAGTCAGTGTTGCCTTAGTTTTGTGCCTGAATGTTGGTGTGGACCCTCCCGATGTGGTGAAGACCACGCCCTGTGCACGCTTGGAATGCTGGATCGATCCTCTGTCGATGGGTCCTCAGAAAGCTCTGGAAACCATCGGTGCAAATTTACAGAAGCAGTACGAGAACTGGCAGCCAAGGGCCCGGTACAAGCAGAGCCTTGACCCAACTGTGGATGAAGTCAAGAAGCTCTGCACGTCCTTACGTCGCAACGCCAAGGAGGAGCGAGTCCTCTTTCACTACAATGGCCACGGGGTGCCCCGGCCCACAGTCAACGGGGAGGTCTGGGTCTTCAACAAGAACTACACGCAGTACATCCCTCTGTCCATATATGACCTGCAGACGTGGATGGGCAGCCCGTCGATCTTCGTCTACGACTGCTCCAATGCTGGCTTGATCGTCAAGTCCTTCAAGCAGTTCGCACTACAGCGGGAGCAGGAGCTGGAGGTAGCTGCAATCAACCCAAATCACCCTCTTGCTCAGATGCCTTTGCCTCCGTCGATGAAAAACTGCATCCAGCTGGCAGCCTGCGAGGCCACCGAGCTGCTGCCCATGATCCCCGACCTCCCGGCTGACCTATTCACCTCCTGCCTCACCACCCCCATCAAGATCGCCCTGCGCTGGTTTTGCATGCAGAAATGTGTCAGTCTGGTGCCTGGCGTCACACTGGATTTGATAGAAAAGATCCCTGGCCGCCTGAACGACAGGAGGACGCCCCTGGGTGAACTGAACTGGATCTTCACAGCCATCACAGACACCATCGCGTGGAACGTGCTCCCCCGGGATCTCTTCCAAAAGCTCTTCAGACAGGACTTGCTGGTGGCTAGTCTGTTTCGAAATTTTTTATTGGCGGAAAGGATTATGAGGTCGTATAACTGCACTCCCGTCAGCAGCCCGCGTCTGCCGCCCACGTACATGCACGCCATGTGGCAAGCCTGGGACCTGGCTGTTGACATCTGTCTGTCTCAGCTGCCGACGATCATCGAGGAAGGCACTGCGTTTCGGCACAGCCCGTTCTTCGCCGAGCAGCTGACCGCATTCCAGGTGTGGCTCACCATGGGCGTGGAGAACCGAAACCCACCCGAACAGCTGCCCATCGTCCTGCAGGTGCTGTTAAGCCAAGTGCACCGGCTGAGAGCATTGGACTTGCTTGGAAGATTTTTGGACCTGGGTCCCTGGGCAGTGAGCCTGGCCTTGTCTGTCGGCATCTTCCCCTACGTGCTGAAGCTGCTCCAGAGCTCGGCCCGAGAGCTGCGGCCACTTCTCGTTTTCATCTGGGCCAAGATCCTCGCAGTGGACAGCTCGTGCCAAGCGGACCTCGTGAAGGACAACGGCCACAAGTACTTCCTGTCGGTCCTGGCGGACCCCTACATGCCAGCTGAACACCGGACCATGACGGCTTTCATTCTCGCCGTGATCGTCAACAGCTATCACACGGGGCAGGAAGCCTGCCTTCAGGGAAACCTCATTGCCATCTGCCTGGAGCAGCTCAACGACCCGCACCCCTTGCTGCGCCAGTGGGTGGCCATCTGCCTCGGCAGGATCTGGCAGAACTTCGACTCGGCGAGGTGGTGCGGCGTGAGGGACAGCGCTCATGAGAAGCTCTACAGCCTCCTCTCCGACCCCATTCCCGAGGTCCGCTGCGCAGCGGTCTTCGCCCTTGGCACGTTCGTGGGCAACTCTGCAGAGAGGACGGACCACTCCACCACCATCGACCACAACGTGGCCATGATGCTGGCCCAGCTGGTCAGCGACGGGAGCCCCATGGTCCGGAAGGAGCTGGTGGTGGCTCTGAGTCATCTTGTGGTTCAGTATGAAAGCAATTTCTGCACCGTGGCCCTGCAGTTCATAGAAGAGGAAAAGAACTACGCCTTGCCTTCTCCAGCAACCACAGAGGGAGGGAGTTTGACCCCAGTGCGAGACAGCCCGTGCACCCCCAGACTTCGTTCTGTGAGCTCCTATGGAAACATCCGTGCTGTCGCCACAGCCAGGAGCCTCAACAAATCTTTGCAGAACCTGAGTTTGACAGAGGAATCTGGTGGCGCGGTGGCGTTCTCCCCCGGAAACCTCAGCACCAGCAGCAGCGCCAGCAGCACCCTGGGCAGCCCCGAGAATGAGGAGCATATCCTGTCCTTCGAGACCATCGACAAGATGCGCCGCGCCAGCTCCTACTCCTCCCTCAACTCCCTCATCGGAGTTTCCTTTAACAGTGTTTACACTCAGATTTGGAGAGTCCTGCTGCACCTGGCTGCTGACCCCTATCCAGAGGTCTCGGACGTGGCCATGAAAGTACTCAACAGCATCGCCTACAAGGCCACCGTGAACGCCCGGCCGCAGCGCGTCCTGGACACCTCCTCCCTCACGCAGTCGGCCCCCGCCAGCCCCACCAACAAGGGCGTGCACATCCACCAGGCGGGGGGCTCCCCTCCGGCGTCCAGCACCAGCAGCTCCAGCCTGACCAACGATGTGGCCAAGCAGCCGGTCAGCCGAGACTTGCCTTCTGGCCGGCCGGGCACCACAGGCCCCGCTGGGGCGCAGTACACCCCTCACTCCCACCAGTTCCCCCGGACACGGAAGATGTTCGACAAGGGCCCAGAGCAGACTGCGGACGACGCGGACGATGCTGCTGGACACAAAAGTTTCATCTCCGCCACGGTGCAGACGGGGTTCTGCGACTGGAGCGCCCGCTATTTTGCCCAGCCCGTCATGAAGATCCCAGAAGAGCACGACCTGGAGAGTCAGATCCGCAAGGAGCGGGAGTGGCGGTTCCTGCGAAACAGCCGTGTCAGGAGGCAGGCCCAGCAAGTCATTCAGAAGGGCATTACGAGATTGGACGACCAAATATTTCTGAACAGGAACCCCGGCGTCCCCTCTGTGGTGAAATTCCACCCCTTCACGCCGTGCATCGCCGTAGCCGACAAGGACAGCATCTGCTTTTGGGACTGGGAGAAAGGGGAGAAGCTGGATTATTTCCACAATGGGAACCCTCGGTACACGAGGGTCACTGCCATGGAGTATCTGAACGGCCAGGACTGCTCGCTTCTGCTGACGGCCACAGACGATGGTGCCATCAGGGTCTGGAAGAATTTTGCTGATTTGGAAAAGAACCCAGAGATGGTGACCGCGTGGCAGGGGCTCTCGGACATGCTGCCAACGACGCGAGGAGCTGGGATGGTGGTGGACTGGGAGCAGGAGACCGGCCTCCTCATGAGCTCAGGAGACGTGCGGATCGTCCGGATCTGGGACACAGACCGTGAGATGAAGGTGCAGGACATCCCTACGGGCGCAGACAGCTGTGTGACGAGTCTGTCCTGTGATTCCCACCGCTCACTCATCGTGGCTGGCCTCGGTGACGGCTCCATCCGCGTCTACGACAGAAGGATGGCACTCAGCGAATGCCGCGTCATGACGTACCGGGAGCACACAGCCTGGGTGGTGAAGGCCTCCCTGCAGAAGCGTCCCGACGGCCACATCGTGAGTGTGAGCGTCAATGGAGATGTGCGCATCTTTGATCCCCGGATGCCTGAGTCGGTAAATGTGCTTCAGATCGTGAAGGGGCTGACGGCCCTGGACATCCACCCCCAGGCGGACCTGATCGCATGTGGCTCCGTCAATCAGTTCACCGCCATCTACAACAGCAGCGGAGAGCTCATCAACAACATCAAGTACTACGACGGCTTCATGGGCCAGCGGGTCGGCGCCATCAGCTGCCTGGCCTTCCACCCGCACTGGCCTCACCTGGCCGTGGGAAGCAACGACTACTACATCTCCGTGTACTCGGTGGAGAAGCGTGTCAGATAG
ORF Protein Sequence MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYHTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLVSDGSPMVRKELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLGSPENEEHILSFETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYKATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRDLPSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMSSGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALDIHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-IP1556-Ab Anti-RPTOR monoclonal antibody
    Target Antigen GM-Tg-g-IP1556-Ag RPTOR protein
    ORF Viral Vector pGMLP005708 Human RPTOR Lentivirus plasmid
    ORF Viral Vector pGMPC000108 Human RPTOR Mammalian (Non-Viral Vector) plasmid
    ORF Viral Vector vGMLP005708 Human RPTOR Lentivirus particle


    Target information

    Target ID GM-IP1556
    Target Name RPTOR
    Gene ID 57521, 74370, 712848, 287871, 101086862, 608378, 507056, 100056244
    Gene Symbol and Synonyms 4932417H02Rik,KOG1,Mip1,mKIAA1303,Rap,Raptor,RGD1311784,RPTOR
    Uniprot Accession Q8N122
    Uniprot Entry Name RPTOR_HUMAN
    Protein Sub-location Introcelluar Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000141564
    Target Classification Not Available

    This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]



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