Human KDM4A/JHDM3A/JMJD2 ORF/cDNA clone-Mammalian (Non-Viral Vector) plasmid (NM_014663)

Cat. No.: pGMPC000642
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human KDM4A/JHDM3A/JMJD2 Non-Viral expression plasmid (overexpression vector) for mouse KDM4A overexpression in unique cell transient transfection and stable cell line development.

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Product Description

Catalog ID pGMPC000642
Gene Name KDM4A
Accession Number NM_014663
Gene ID 9682
Species Human
Product Type Mammalian (Non-Viral Vector) plasmid (overexpression)
Insert Length 3195 bp
Gene Alias JHDM3A,JMJD2,JMJD2A,TDRD14A
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGCTTCTGAGTCTGAAACTCTGAATCCCAGTGCTAGGATAATGACCTTTTATCCAACTATGGAAGAGTTCCGAAACTTCAGTAGATACATTGCCTACATTGAATCCCAAGGAGCTCATCGGGCAGGGCTAGCCAAGGTTGTTCCTCCAAAAGAGTGGAAGCCACGAGCATCCTATGATGACATTGATGATTTGGTCATTCCTGCCCCCATTCAACAGCTGGTGACGGGGCAGTCTGGCCTCTTTACTCAGTACAACATACAGAAGAAAGCCATGACTGTTCGAGAGTTCCGCAAGATAGCCAATAGCGATAAGTACTGTACCCCACGCTATAGTGAGTTTGAAGAGCTCGAGCGGAAATACTGGAAAAATCTTACATTCAATCCTCCAATCTATGGTGCAGATGTGAATGGTACCCTCTATGAAAAGCATGTTGATGAGTGGAATATTGGCCGGCTGAGAACAATCCTGGACTTGGTGGAAAAGGAGAGTGGGATCACCATTGAGGGTGTGAACACCCCATACCTGTACTTTGGCATGTGGAAGACATCCTTTGCTTGGCACACTGAAGACATGGACCTCTACAGCATCAACTACCTGCACTTTGGAGAACCAAAGTCCTGGTACTCTGTTCCACCTGAGCATGGAAAGCGGTTGGAACGCCTCGCCAAAGGCTTTTTCCCAGGAAGTGCTCAAAGCTGTGAGGCATTTCTCCGCCACAAGATGACCCTGATTTCCCCGTTAATGCTGAAGAAATATGGAATTCCCTTTGACAAGGTGACTCAAGAGGCTGGAGAGTTTATGATCACTTTCCCTTATGGTTACCATGCCGGCTTTAACCATGGTTTTAACTGTGCGGAGTCTACCAATTTTGCTACCCGTCGGTGGATTGAGTACGGCAAGCAAGCTGTGCTGTGCTCCTGTAGAAAGGACATGGTGAAGATCTCCATGGATGTGTTTGTGAGAAAGTTCCAGCCAGAAAGGTACAAACTTTGGAAAGCTGGGAAGGACAACACAGTTATTGACCATACTCTGCCCACGCCAGAAGCAGCTGAGTTTCTTAAGGAGAGTGAACTGCCTCCAAGAGCTGGCAACGAGGAGGAGTGCCCAGAGGAGGACATGGAAGGGGTGGAGGATGGAGAGGAAGGAGACCTGAAGACAAGCCTGGCCAAGCACCGAATAGGGACAAAGAGGCACCGAGTTTGTCTTGAAATACCACAGGAGGTGAGTCAGAGTGAGCTCTTCCCCAAGGAGGATCTGAGTTCTGAGCAGTATGAGATGACGGAGTGCCCGGCAGCCCTCGCCCCTGTGAGGCCCACCCATAGCTCTGTGCGGCAAGTTGAGGATGGTCTTACCTTCCCAGATTATTCTGACTCCACTGAAGTCAAATTTGAAGAGCTTAAAAATGTCAAACTAGAAGAGGAGGATGAGGAGGAAGAACAAGCAGCAGCTGCCTTGGATCTTTCTGTGAATCCTGCGTCTGTAGGGGGACGCCTTGTCTTCTCAGGCTCCAAAAAGAAATCATCTTCTAGCCTGGGCTCTGGCTCTTCACGGGATTCTATCTCTTCTGATTCAGAAACTAGTGAGCCTCTCTCCTGCCGAGCCCAAGGGCAAACGGGAGTTCTCACTGTGCACAGTTATGCCAAAGGGGATGGCAGGGTCACTGTGGGAGAGCCATGCACGAGGAAGAAAGGAAGCGCCGCTAGAAGTTTCAGTGAGCGGGAGCTGGCAGAGGTTGCAGATGAATACATGTTTTCCCTAGAAGAGAATAAGAAGTCCAAGGGACGCCGTCAGCCTTTAAGCAAGCTCCCCCGCCATCACCCACTTGTGCTGCAGGAGTGTGTCAGTGATGATGAGACATCTGAACAGCTGACCCCTGAGGAAGAGGCTGAGGAGACAGAGGCCTGGGCCAAGCCTCTGAGCCAACTGTGGCAGAACCGACCTCCAAACTTTGAGGCTGAGAAGGAATTCAATGAGACCATGGCCCAACAGGCCCCTCACTGCGCTGTCTGTATGATCTTCCAGACTTATCATCAGGTTGAATTTGGAGGCTTTAATCAGAACTGTGGAAATGCTTCAGATTTAGCCCCCCAGAAGCAGAGGACCAAGCCATTGATTCCAGAAATGTGCTTCACTTCGACTGGCTGCAGCACGGACATCAACCTTTCTACTCCTTATCTTGAGGAGGATGGCACCAGCATACTCGTTTCCTGCAAGAAGTGCAGCGTCCGGGTCCATGCCAGTTGCTATGGGGTCCCCCCTGCAAAGGCTTCTGAAGACTGGATGTGTTCTCGGTGTTCAGCCAATGCCCTAGAGGAGGACTGCTGTTTATGCTCATTACGAGGAGGGGCCCTGCAGAGAGCAAATGATGACAGGTGGGTCCACGTTTCATGTGCTGTGGCAATTCTGGAAGCAAGGTTTGTCAACATTGCAGAAAGAAGTCCGGTGGATGTGAGCAAAATCCCCCTGCCCCGCTTCAAACTGAAATGTATCTTCTGTAAGAAGCGGAGGAAAAGAACTGCTGGCTGCTGTGTGCAGTGTTCTCACGGCCGCTGCCCAACTGCCTTCCATGTGAGCTGCGCCCAGGCTGCCGGTGTGATGATGCAGCCTGACGACTGGCCTTTTGTGGTCTTCATTACCTGCTTTCGGCACAAGATTCCTAATTTGGAGCGTGCCAAGGGGGCCTTGCAAAGCATCACTGCAGGCCAGAAAGTCATTAGCAAGCATAAGAACGGGCGCTTCTACCAGTGTGAAGTGGTCAGGCTCACCACCGAGACCTTCTATGAAGTCAACTTTGATGATGGCTCCTTCAGCGACAATCTTTATCCTGAGGACATAGTGAGCCAGGACTGTCTCCAGTTTGGTCCTCCTGCTGAAGGGGAAGTGGTCCAAGTGAGATGGACAGACGGCCAAGTCTATGGAGCCAAGTTTGTGGCCTCCCACCCTATCCAAATGTACCAGGTGGAGTTTGAGGATGGCTCACAACTTGTGGTTAAGAGAGATGATGTATACACACTGGATGAAGAGCTTCCCAAGAGAGTCAAATCTAGACTGTCAGTAGCCTCAGACATGCGCTTCAATGAGATTTTCACAGAGAAAGAGGTTAAGCAAGAAAAGAAACGGCAACGAGTTATCAACTCAAGATACCGGGAAGATTATATTGAGCCTGCACTATACCGGGCCATCATGGAGTAG
ORF Protein Sequence MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRASYDDIDDLVIPAPIQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTLYEKHVDEWNIGRLRTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYSVPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFNHGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEAAEFLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKTSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDLSSEQYEMTECPAALAPVRPTHSSVRQVEDGLTFPDYSDSTEVKFEELKNVKLEEEDEEEEQAAAALDLSVNPASVGGRLVFSGSKKKSSSSLGSGSSRDSISSDSETSEPLSCRAQGQTGVLTVHSYAKGDGRVTVGEPCTRKKGSAARSFSERELAEVADEYMFSLEENKKSKGRRQPLSKLPRHHPLVLQECVSDDETSEQLTPEEEAEETEAWAKPLSQLWQNRPPNFEAEKEFNETMAQQAPHCAVCMIFQTYHQVEFGGFNQNCGNASDLAPQKQRTKPLIPEMCFTSTGCSTDINLSTPYLEEDGTSILVSCKKCSVRVHASCYGVPPAKASEDWMCSRCSANALEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCIFCKKRRKRTAGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALQSITAGQKVISKHKNGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVSQDCLQFGPPAEGEVVQVRWTDGQVYGAKFVASHPIQMYQVEFEDGSQLVVKRDDVYTLDEELPKRVKSRLSVASDMRFNEIFTEKEVKQEKKRQRVINSRYREDYIEPALYRAIME

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-T18904-Ab Anti-KDM4A monoclonal antibody
    Target Antigen GM-Tg-g-T18904-Ag KDM4A protein
    ORF Viral Vector pGMLP001677 Human KDM4A Lentivirus plasmid
    ORF Viral Vector pGMPC000642 Human KDM4A Mammalian (Non-Viral Vector) plasmid
    ORF Viral Vector vGMLP001677 Human KDM4A Lentivirus particle


    Target information

    Target ID GM-T18904
    Target Name KDM4A
    Gene ID 9682, 230674, 701272, 313539, 101081651, 482530, 512622, 100053154
    Gene Symbol and Synonyms D4Ertd222e,JHDM3A,JMJD2,JMJD2A,KDM4A,mKIAA0677,TDRD14A
    Uniprot Accession O75164
    Uniprot Entry Name KDM4A_HUMAN
    Protein Sub-location Introcelluar Protein
    Category Therapeutics Target
    Disease Not Available
    Gene Ensembl ENSG00000066135
    Target Classification Not Available

    This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]



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