2019 nCoV (SARS CoV2 coronavirus) Spike(S1+S2)-RBD N501Y mutation (B.1.1.7 lineage RBD) Pre-made adenovirus vector
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|Catalog No.||Package||Price(In USD)||Qty (Quantity)||Sum(In USD)|
|GMV-V-2019nCoV-105||In vitro grade: 1E+10PFU (1E+10pfu/ml×1ml)||3390|
|GMV-V-2019nCoV-105||In vitro grade: 5E+10PFU (1E+10pfu/ml×5ml)||4990|
|GMV-V-2019nCoV-105||In vitro grade: 1E+11PFU (1E+10pfu/ml×10ml)||9090|
|GMV-V-2019nCoV-105||In vivo grade: 1E+11PFU (1E+11pfu/ml×1ml)||Inquiry.|
|2019 nCoV related Gene||Spike(S1+S2)-RBD N501Y mutation (B.1.1.7 lineage RBD)|
|Species Host||SARS-COV-2 B.1.1.7 lineage|
|Antibiotic in Mammalian cell||null|
|Codon Optimized||Codon Optimized for mamamlian|
|Gene Accession Number||QHD43416.1|
|Application||Adenovirus-based 2019nCoV vaccine; Adenovirus production; Mammalian cells expression|
SARS-CoV-2 spike-N501Y mutation
The world is in midst of the COVID-19 pandemic caused by SARS-CoV-2 (2019nCoV) infection. The Spike protein (S-protein) of SARS-CoV-2 (2019nCoV) mediates receptor (ACE2) binding and cell entry and is the dominant target of the immune system. Most mutations and deletions of SARS-CoV-2 occur in the coronavirus spike protein. Spike position N501, one of the key contact residues in the receptor-binding domain (RBD), and experimental data suggest mutation N501Y can increase ACE2 receptor affinity (Starr et al. 2020). N501Y has also been associated with increased infectivity and virulence in a mouse model (Gu et al. 2020).
Recently a novel SARS-COV-2 (2019nCOV) lineage, the B.1.1.7 lineage, with serials of site mutation, shows stronger infection ability in the UK. In SARS-COV-2 B.1.1.7 lineage, most mutations and deletions occur in the coronavirus spike protein. These mutantions also include SARS-CoV-2 spike- mutation. N501Y mutation is also found in the South Africa 501Y.V2 lineage.
The pre-made gene ORF vector of Spike(S1+S2)-N501Y mutation is Codon Optimized for mamamlian and can be used for mammalian expression.
GeneMedi also provides pre-made the lentivirus, adenovirus and AAV vector for the gene ORF plasmids of 2019 nCoV (SARS2 coronavirus).
GeneMedi codon-optimized spike mammalian expression vector for SARS-COV-2 (2019nCOV) spike-N501Y mutation
GeneMedi pseudotype virus (pseudovirus) of SARS-COV-2 (2019nCOV) Spike-N501Y mutation
1 Gu, Hongjing, Qi Chen, Guan Yang, Lei He, Hang Fan, Yong-Qiang Deng, Yanxiao Wang, et al. 2020. “Adaptation of SARS-CoV-2 in BALB/c Mice for Testing Vaccine Efficacy.” Science 369 (6511): 1603–7.
2 Starr, Tyler N., Allison J. Greaney, Sarah K. Hilton, Daniel Ellis, Katharine H. D. Crawford, Adam S. Dingens, Mary Jane Navarro, et al. 2020. “Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding.” Cell 182 (5): 1295–1310.e20.