Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutant (B.1.526 lineage; 20A.EU2 lineage)

Order information

Package	Catalog No.	Price(In USD)	Qty (Quantity)	Sum(In USD)
1mg	GMP-V-2019nCoV-Smu-018-1mg	5990
10mg	GMP-V-2019nCoV-Smu-018-10mg	38790
100mg	GMP-V-2019nCoV-Smu-018-100mg	258000
≥100mg	GMP-V-2019nCoV-Smu-018-xmg	Inquiry
			Shipping Cost:	760.00
			Total:

Description

Accession Number	QHD43415.1
Expression platform	2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Isotypes	Mamamlian (human cell)
Tag	C-His
Products description	Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutation (B.1.526 lineage; 20A.EU2 lineage) was expressed in mammanlian cell (human cells) expression system with 6 HIS tag at the C-terminus.
Bioactivity validation	ACE2 binding; Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays;
Purity	Purity: ≥95% (SDS-PAGE)
Application	Spike protein & ACE2 competition binding assay for efficacy evaluation of COVID-19 vaccines and therapeutic antibodies. Immunogen in Elisa,lateral-flow tests,and other immunoassays; Standard substance The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass	26kDa
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutant (B.1.526 lineage; 20A.EU2 lineage)

The world is in midst of the COVID-19 pandemic. Recently a new SARS-CoV-2 (2019nCOV)lineage (B.1.526) emerged in New York and spread rapidly. The New York variant B.1.526 lineage is characterised by six lineage-defining mutations in the spike protein, including D614G mutation and one at important residues in the receptor-binding domain (S477N or E484K)1.The combination of E484K or S477N with a D253G mutation of the B.1.526 lineage that might confer immune escape and the increased number of COVID-19 cases¹

Mutations of Spike protein in SARS-COV-2 (2019nCOV) B.1.526 lineage spread in New York

One spike mutation is at key residues in the RBD (S477N or E484K), two are in the N-terminal domain (T95I and D253G) and one is in loop 2 (A701V) ¹.