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Package Catalog No. Price(In USD) Qty (Quantity) Sum(In USD)
1mg GMP-V-2019nCoV-Smu-018-1mg 5990
10mg GMP-V-2019nCoV-Smu-018-10mg 38790
100mg GMP-V-2019nCoV-Smu-018-100mg 258000
≥100mg GMP-V-2019nCoV-Smu-018-xmg Inquiry
Shipping Cost: 760.00
Total:



Description

Accession Number QHD43415.1
Host specics 2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Source (Expression Host)Mamamlian (human cell)
TagC-His
Products descriptionRecombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutation (B.1.526 lineage; 20A.EU2 lineage) was expressed in mammanlian cell (human cells) expression system with 6 HIS tag at the C-terminus.
Bioactivity validation ACE2 binding;
Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays;
PurityPurity: ≥95% (SDS-PAGE)
ApplicationSpike protein & ACE2 competition binding assay for efficacy evaluation of COVID-19 vaccines and therapeutic antibodies.
Immunogen in Elisa,lateral-flow tests,and other immunoassays;
Standard substance
The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass26kDa
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutant (B.1.526 lineage; 20A.EU2 lineage)

The world is in midst of the COVID-19 pandemic. Recently a new SARS-CoV-2 (2019nCOV)lineage (B.1.526) emerged in New York and spread rapidly. The New York variant B.1.526 lineage is characterised by six lineage-defining mutations in the spike protein, including D614G mutation and one at important residues in the receptor-binding domain (S477N or E484K)1.The combination of E484K or S477N with a D253G mutation of the B.1.526 lineage that might confer immune escape and the increased number of COVID-19 cases1

Mutations of Spike protein in SARS-COV-2 (2019nCOV) B.1.526 lineage spread in New York

One spike mutation is at key residues in the RBD (S477N or E484K), two are in the N-terminal domain (T95I and D253G) and one is in loop 2 (A701V) 1.

Spike Mutation in SARS-COV-2 (2019nCOV) B.1.526 lineage
SP L5F
Spike-S1 Subunit
N Terminal
T95I
D253G Important residues mutation
Spike-RBD S477N or E484K Important residues mutation
Spike-S1 Subunit D614G A popular mutation in different new SARS-CoV-2 lineage
Spike-S2 Subunit A701V