Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutant (B.1.526 lineage; 20A.EU2 lineage)
Cat No.: GMP-V-2019nCoV-Smu-018
Order information
Package | Catalog No. | Price(In USD) | Qty (Quantity) | Sum(In USD) |
---|---|---|---|---|
1mg | GMP-V-2019nCoV-Smu-018-1mg | 5990 | ||
10mg | GMP-V-2019nCoV-Smu-018-10mg | 38790 | ||
100mg | GMP-V-2019nCoV-Smu-018-100mg | 258000 | ||
≥100mg | GMP-V-2019nCoV-Smu-018-xmg | Inquiry | ||
Shipping Cost: | 760.00 | |||
Total: | ||||
Description
Accession Number | QHD43415.1 |
Expression platform | 2019-nCoV(SARS-CoV-2, SARS2-coronavirus) |
Isotypes | Mamamlian (human cell) |
Tag | C-His |
Products description | Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutation (B.1.526 lineage; 20A.EU2 lineage) was expressed in mammanlian cell (human cells) expression system with 6 HIS tag at the C-terminus. |
Bioactivity validation | ACE2 binding; Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays; |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Spike protein & ACE2 competition binding assay for efficacy evaluation of COVID-19 vaccines and therapeutic antibodies. Immunogen in Elisa,lateral-flow tests,and other immunoassays; Standard substance The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay. |
predicted Molecular Mass | 26kDa |
Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD S477N mutant (B.1.526 lineage; 20A.EU2 lineage)
The world is in midst of the COVID-19 pandemic. Recently a new SARS-CoV-2 (2019nCOV)lineage (B.1.526) emerged in New York and spread rapidly. The New York variant B.1.526 lineage is characterised by six lineage-defining mutations in the spike protein, including D614G mutation and one at important residues in the receptor-binding domain (S477N or E484K)1.The combination of E484K or S477N with a D253G mutation of the B.1.526 lineage that might confer immune escape and the increased number of COVID-19 cases1Mutations of Spike protein in SARS-COV-2 (2019nCOV) B.1.526 lineage spread in New York
One spike mutation is at key residues in the RBD (S477N or E484K), two are in the N-terminal domain (T95I and D253G) and one is in loop 2 (A701V) 1.
Spike Mutation in SARS-COV-2 (2019nCOV) B.1.526 lineage | ||
SP | L5F | |
Spike-S1 Subunit N Terminal |
T95I | |
D253G | Important residues mutation | |
Spike-RBD | S477N or E484K | Important residues mutation |
Spike-S1 Subunit | D614G | A popular mutation in different new SARS-CoV-2 lineage |
Spike-S2 Subunit | A701V |