Pre-made AAV Production Service for Quick Delivery

  • Product Overview
  • Manuals & MSDS
  • FAQs
  • Citations and references
  • Recommended products

Order Information

  • Step 1:
    AAV vector construction
  • Step 2:
    AAV packaging
  • Step 3:
    Custom Order confirmation

If you only konw the gene sequence,please contact us for the quotation

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Buy Product Name NMID Flurescent Label Promoter Catalog NO.
Plasmid Validation (qRCR) Plasmid Validation (Western Blot)

Note: If the selection of AAV serotype and viral packages listed above do not fully meet your requirements, please contact us.

Buy AAV Vector Production Scale Final Deliverables Lead Time(workday) Price(in USD)

Introduction to Pre-made Adeno Associated Virus (AAV) Production Service

Adeno associated virus (AAV) is one kind of human parvovirus. Recombinant AAV vectors can efficiently transfect various cell types, including dividing and quiescent cells, and induce persistent gene expression in vivo without integrating into host genome and causing any disease. These features make AAV an attractive candidate in the application of viral constructs for gene therapy and human disease model establishment. To date, AAV has been proved as the most excellent gene therapy vector. Over 204 clinical trials have been carried out using AAV vectors for gene delivery, and promising gene therapy outcomes have been achieved from clinical trials for a great number of diseases. Genemedi has launched a comprehensive AAV production service.

Genemedi offers a comprehensive library of pre-made AAV plasmid, containing full-length ORFs, which have been confirmed by sequencing, are available for direct AAV production, which may greatly shorten the supply period of AAV virus, saving more than 2 weeks.

AAV serotypes and their respective Tropism
AAV SErotype Tissue tropism
CNS Retina Lung Liver Pancreas Kidney Heart Muscle
AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV7
AAV8
AAV9
AAV-DJ
AAV-DJ/8
AAV-Rh10
AAV-retro
AAV-PHP.B
AAV8-PHP.eB
AAV-PHP.S

Properties

Genemedi AAV Particles
Quantity/Unit Vials
Form Frozen form
Suitable Types of Infection In vivo infection in animals
Sipping and Storage Guidelines Shipped by dry ice, stored at -80 ℃, effective for 1 year. Avoid repeatedly freezing and thawing.
Titer > 1*10^12v.g/ml

Advantages

1. Safety. The wild type Adeno Associated Virus (AAV) has not currently been known to cause disease, and further security of recombinant AAV is ensured after removal of most AAV genome elements.

2. Low immunogenicity. AAV causes a very mild immune response, lending further support to its apparent lack of pathogenicity.

3. Broad range of host and specificity targeting. AAV has the ability to infect both dividing and quiescent cells, allowing genetic material to be delivered to a highly diverse range of cell types. More than 12 AAV serotypes and a variety of capsid engineered AAV vectors can be selected according to their tissue tropisms.

4. Long-term stable expression. Long term and stable expression of genes in vivo can be mediated by AAV.

5. Stable physical properties. AAV is still alive at 60 ℃ and resistant to chloroform.

6. Short supply period. More than 10 thousand transgene plasmids are available for direct AAV packaging which may save more than 2 weeks.

Applications and Figures

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Figure 1
Transduction results of various AAV serotypes by Genemedi. Selected results of AAV transduction in the target organ, such as heart (A), liver (B), retina (C), whole brain (D), fat (E).

Quality control description

AAV capsid contains VR1 82kDa, VR2 72kDa and VR3 62kDa, which can be detected using the method of polyacrylamide gel electrophoresis (PAGE) followed by silver staining or Coomassie blue staining. Pure AAV should display only three major protein bands, such as shown in Figure 2.

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Figure 2. PAGE for quality testing example of AAV by Genemedi. Only 3 protein bands can be seen in the purified AAV virus samples.

Technical Documents

1. For further information about AAV administration and transduction results, please see the pdf downloadAAV(adeno associated virus) User Manual.

Frequently Asked Questions(FAQs)

1. How can I choose the optimal AAV serotypes for my in vivo study?
Answer

Genemedi has launched a comprehensive AAV production service. More than 12 AAV serotypes and a variety of capsid engineered AAV vectors are available for targeting different tissues and organs. Different AAV serotypes have different tissue tropism in vivo, the common serotypes and their tropism are listed in the above table 1. Therefore, you can select the most suitable AAV serotype for your study according to this table. For example, if you’d like to transfect your target gene into mouse ears, the AAV-Anc80 will be your best choice.

For your optimal AAV serotypes screening, Genemedi can also customize an AAV serotype testing kit with recommended serotypes of your choice.

2. How much DNA do I need to provide for Custom AAV without DNA amplification?
Answer

You will need to provide purified plasmid DNA at a concentration of 0.5ug/ul or more.
50 ug DNA needed for Custom AAV production service without DNA amplification (10^9 GC/ml).
200 ug DNA needed for Custom AAV production service without DNA amplification (10^12 GC/ml).
300 ug DNA needed for Custom AAV production service without DNA amplification (10^13 GC/ml).

3. What's the difference of AAV titer unit between GC/ml and vg/ml?
Answer

The AAV titer unit GC/ml and vg/ml can be used interchangeably, based on qPCR method.

4. Why can’t I see the expression of GFP after AAV infection in my cell line?
Answer

AAV serotypes selection is an important parameter which may affect the transduction ability of AAV particles. Thus, it is necessary to determine which serotype works best for your cell line. For instance, serotype 5 limits its transduction ability on most cell types. For detailed information, you can refer to our technical sheet "AAV - General Guideline to Serotype Selection".

5. Does Genemedi’s AAV preparations belong to in-vivo grade?
Answer

Extensive purification steps guarantee the high quality of our viral particles, which are ready to be administrated for in-vivo models. For detailed references, please see the in-vivo infection data tested by another independent lab { www.genemedi.net }.

6.What are the QC ( quality control) methods for testing AAV in Genemedi?
Answer

We provide qPCR-based titer as the primary method to determine whether the packaging/purification process is successful or not. If your virus has GFP or RFP reporter, we also perform virus infectivity testing in HEK293T cell line.

7.Can I utilize different serotypes of AAV virus in the same equipment to keep the infected cells?
Answer

There is no problem with using different serotypes in the same equipment, as long as the handler takes the basic precautions to avoid cross-contamination.

8. What is the difference between brain localization of gene expression after injection of AAV or lentiviral vectors?
Answer

Lentiviral particles don't spread well after stereotaxic injection into brain because the particles are relatively large (90nm~100nm). In contrast, AAV particles spread more readily due to smaller size (20nm~100nm). As mentioned, it has been stated that some AAV serotypes spread better than others in brain, for example, AAV5 is reported to spread exceptionally well when injected into striatum. Lastly, pretreatment with mannitol to your animal about 15 min ahead of viral injection has been reported to aid in spread of viral particles in the brain.

9. Do AAVs tranduce axons passing through a region of interest?
Answer

AAVs are able to infect axon terminals and produce retrograde transport (towards the cell body). The slightly longer answer is that this process is highly biased based on the serotype you are using. There are a number of papers reporting AAV6 (and maybe AAV6.2) is the main.

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Reference

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