Tissue-specific AAV-Cre for in vivo study

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Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre)

Cat No. Pre-made Cre Viral vector Protomer Promoter Tissue Specificity Promoter Tissue
Specificity characteristics
GMV-AdCre-01Ad-CMV-CreAdenovirusCMV Total Total
GMV-AdCre-02Ad-CMV-Cre-GFPCMV Total Total

GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option:
· AAV1-Cre
· AAV2-Cre
· AAV2 variant (Y444F)-Cre
· AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre
· AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre
· AAV2 variant(AAV2.7m8)-Cre
· AAV5-Cre
· AAV6-Cre
· AAV8-Cre
· AAV8-1m-Cre
· AAV8-2m-Cre
· AAV8 variant (Y733F, Y447F, Y275)-Cre
· AAV9-Cre
· AAV-Rh.10-Cre
· AAV-DJ-Cre
· AAV-DJ/8-Cre
· AAV-Retro (Retrograde)-Cre
· AAV9-PHP.B-Cre
· AAV9-PHP.A-Cre
· AAV9-PHP.eB-Cre
· AAV9-PHP.S-Cre
CMV Total Total
GMV-AAV9Cre-02AAV9-CMV-Cre-ZsGreen CMV Total Total
GMV-AAV9Cre-03AAV9-CAG-Cre CAG Total Total
GMV-AAV9Cre-04AAV9-CAG-Cre-ZsGreen CAG Total Total
GMV-AAV9Cre-05AAV9-Syn-Cre-EGFP synapsin (SYN) NeuroNeuron-specific promoter
GMV-AAV9Cre-06AAV9-Syn-Cre synapsin (SYN) NeuroNeuron-specific promoter
GMV-AAV9Cre-07AAV9-CaMKII-Cre-EGFP CaMKIINeuroForebrain glutamate neuron-specific promoter
GMV-AAV9Cre-08AAV9-CaMKII-Cre CaMKIINeuroForebrain glutamate neuron-specific promoter
GMV-AAV9Cre-09AAV9-GFAP-Cre-EGFP GFAPNeuroAstrocyte specific promoter
GMV-AAV9Cre-10AAV9-GFAP-Cre GFAPNeuroAstrocyte specific promoter
GMV-AAV9Cre-11AAV9-cTNT-Cre cTnTheartCardiomyocyte specific promoter
GMV-AAV9Cre-12AAV9-cTNT-Cre-EGFP cTnTheartCardiomyocyte specific promoter
GMV-AAV9Cre-13AAV9-TBG-Cre TBGliverHepatic specific promoter
GMV-AAV9Cre-14AAV9-TBG-Cre-ZsGreen TBGliverHepatic specific promoter
GMV-AAV9Cre-15AAV9-TIE-Cre TIEendothelialEndothelial-specific promoter
GMV-AAV9Cre-16AAV9-cd68-Cre cd68NeuroMicroglia-specific promoter
GMV-AAV9Cre-17AAV9-c-fos-Cre c-fosNeuroExcitatory neuron promoter
GMV-AAV9Cre-18AAV9-mecp2-Cre mecp2NeuroShorter neuron-specific promoter
GMV-AAV9Cre-19AAV9-MHCK7-Cre MHCK7muscleMuscle specific promoter
GMV-AAV9Cre-20AAV9-Rpe65-Cre Rpe65retinaRetinal specific promoter
GMV-AAV9Cre-21AAV9-sm22a-Cre sm22amuscleSmooth muscle specific promoter
GMV-AAV9Cre-22AAV9-Spc5-12-Cre Spc5-12muscleMyocyte specific promoter
GMV-AAV9Cre-23AAV9-Ta1-Cre TalNeuroEarly neuron-specific promoter
GMV-LvCre-01Lv-CMV-CreLentivirusCMV Total Total
GMV-LvCre-02Lv-CMV-Cre-zsgreenCMV Total Total


Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI). To date, numerous systems, derived from the basic Cre-loxP system, have been developed to precisely control gene expression at desired time or cells. Here, we briefly introduce Cre-loxP system, and give a summary about its applications, especially viral vectors-mediated Cre expression (such as AAV-Cre or Ad-Cre) for in vitro or in vivo studies.

Tissue-specific AAV-Cre for in vivo study

As the most excellent gene therapy vector, recombinant AAV can mediate long-term stable expression of target genes in vitro/vivo with broad range of host and low immunity. Over the past decades, numerous AAV serotypes have been discovered or developed, and each serotype has its special tropism. For instance, AAV2 can moderately transduce several tissue types, including central nervous system, liver, muscle, and lung. Similarly within the CNS, AAV1 and AAV5 show higher transduction frequencies than AAV2 in all injected regions [74, 75], while AAV4 just appears to transduce specific cell types, such as the astrocytes and ependyma in the subventricular zone [76]. In skeletal muscle cells, AAV1, AAV6 and AAV7 is reported to present very high transduction rate [77-80], while AAV8 exhibits transduction ability with super efficiency not only in hepatocytes but also in other organs [78, 81, 82]. In addition to skeletal muscle cells, AAV6 has been also reported to mediate more efficient transduction of airway epithelial cells in mouse lungs compared to that of AAV2 [81], which may supply AAV6 with more significant advantages over AAV2 for gene therapy of lung diseases like cystic fibrosis considering the lower immunogenicity of AAV6 than of AAV2 [78, 82].

GeneMedi holds the expertise at AAV production, you can find more information and protocols about AAV on this website: https://www.genemedi.net/i/aav-packaging.

Combined with Cre-loxP system, AAV containing Cre (AAV-Cre) can help mediate GOI conditional KO in specific tissues or organs based on its special tissue trophism (Fig. 5). This technology has been widely applied into several research fields, especially for the gene function studies in neurosciences. Some of examples are listed in Table 2.

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Figure 5. Generation schematics for AAV-mediated tissue-specific GOI deletion. About two weeks post-infection of AAV-Cre viruses, the transgenic mice with floxed DNA will express Cre in specific tissue based on the AAV tropism. Then, in this specific tissue, Cre recombinase will bind and excise the loxP sites, thus inactivate the GOI.

Table 2. Examples of tissue-specific KO via AAV-Cre/loxP system
AAV serotype Tissue/organ Delivery route Gene Reference
AAV9Hippocampus or central amygdalaStereotaxic microinjectionIRs and IGF1Rs[11]
AAV9AstrocyteIn vitro transductionIGFR[83]
AAV2/5Large DRG neuronsIntrathecal injectionNa(V)1.6[84]
AAV9Spinal dorsal horn neuronsIntrathecal injectionSIRT1[85]
AAV2Cortical neuronsUnilateral intracortical injectionsPTEN[86]
AAV5Forebrain excitatory neurons Stereotaxic microinjectionSIRT1[87]
AAV2/1MuscleIntramuscular injectionMtm1[88]
AAV2Ventral tegmental area, VTAIntra-VTA infusionGabrd[89]
AAV8Sensorimotor cortex, hippocampus, and spinal cordIntra-sensorimotor cortex injectionPTEN[90]
AAV2/5mesolimbic Ventral tegmental area (VTA), NAc core and central amygdala (CeA)Intracerebroventricular (ICV) injectionLepr[91]
AAV9Dentate gyrus (DG)Intra-DG injectionShh[92]
AAV8LiverLateral tail vein injectionHKDC1[93]
AAV1HypothalamusStereotaxic microinjectionAC3[94]
Additionally, tissue-specific promoter is also used to drive Cre expression to strengthen the tissue specificity of GOI knockout. Several tissue-specific promoters, within the loading capacity of AAV vectors, are listed in the following table 3.

Table 3. Tissue-specific promoters in AAV-Cre
Tissue/organ Promoter Specificity Promoter size Species
mecp2Short neuron0.2kbmouse
TUBA1ADeveloping neuron1.2kbhuman
c-fosExcitatory neuron1.7kbmouse
CaMKIIaGlutamate neurons in the forebrain1.1kbmouse
hVGATGABAergic interneurons1.8kbhuman
Slc6a3Dopamine and serotonin neurons1.2kbmouse
CNPSchwann cell 1.5kbmouse
SM22aSmooth muscle0.5kbmouse
SkeletonCOL2A1 Chondrocyte 1.2kbmouse
LungSP-CLung epithelial cells0.3kbmouse
LiverTBGLiver cells0.4kbhuman
PanCreasPDX1PanCreas cells1.2kbmouse
FatFABP4Fat cells1.6kbmouse
EndothelialTIEEndothelial cells1.2kbmouse
CorneumK14Keratinocyte 1.5kbmouse
Retinarpe65Retinal cells 0.7kbmouse
GeneMedi offers kinds of pre-made AAV-Cre particles in different serotypes driven by optional promoters. Click here for more information about GeneMedi’s ready-to-use AAV-Cre:https://www.genemedi.net/i/Cre-products


The Cre-loxP system, especially inducible tissue-specific knockout and viral vector-mediated Cre expression, has been highly utilized in genetics and cell biology research. Nevertheless, it seems that Cre-loxP system will continue to be prevalent in current and future research studies. GeneMedi is proficient in viral vector development and offers kinds of viral vector-based Cre tools, we can provide the best services and products if required.


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