Human ZBTB33/ZNF-kaiso/ZNF348 ORF/cDNA clone-Adenovirus plasmid (NM_001184742.1)

Cat. No.: pGMAD000086
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human ZBTB33/ZNF-kaiso/ZNF348 adenoviral expression plasmid for ZBTB33 adenovirus packaging, ZBTB33 adenovirus.

Our GM-Adenovirus vector is optimized with the GMVC-modified Adeasy adenovirus packaging system. Find more about the GMVC-modified adenovirus packaging system.

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Product Description

Catalog ID pGMAD000086
Gene Name ZBTB33
Accession Number NM_001184742.1
Gene ID 10009
Species Human
Product Type Adenovirus plasmid (overexpression)
Insert Length 2019 bp
Gene Alias ZNF-kaiso,ZNF348
Fluorescent Reporter Null
Mammalian Cell Selection Null
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGGCAGTCTGCTGAACTCCTTGAATGAGCAACGTGGCCATGGACTCTTCTGTGATGTTACCGTTATTGTGGAAGACCGAAAATTCCGGGCTCACAAGAATATTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTCTTCTCTGTTGCTGGGCAAGTTGTTGAACTGAGCTTTATAAGAGCAGAGATCTTTGCAGAAATTCTCAATTATATCTATAGTTCTAAAATTGTTCGTGTTAGATCAGATTTGCTTGATGAGTTAATTAAATCAGGGCAGTTATTAGGAGTGAAATTTATAGCAGAGCTTGGTGTCCCATTGTCACAGGTTAAAAGCATCTCAGGTACAGCGCAGGATGGTAATACTGAGCCTTTACCTCCTGATTCTGGTGACAAGAACCTTGTAATACAGAAATCAAAAGATGAAGCCCAAGATAATGGGGCTACTATAATGCCTATTATAACAGAGTCTTTTTCATTATCTGCCGAAGATTATGAAATGAAAAAGATCATTGTTACCGATTCTGATGATGATGATGATGATGTCATTTTTTGCTCCGAGATTCTGCCCACAAAGGAGACTTTGCCGAGTAATAACACAGTGGCACAGGTCCAATCTAACCCAGGCCCTGTTGCTATTTCAGATGTTGCACCTAGTGCTAGCAATAACTCGCCCCCTTTAACAAATATCACACCTACTCAGAAACTTCCTACTCCTGTGAATCAGGCAACTTTGAGCCAAACACAAGGAAGTGAAAAATTGTTGGTATCTTCAGCTCCAACACATCTGACTCCCAATATTATTTTGTTAAATCAGACACCACTTTCTACACCACCAAATGTCAGTTCTTCACTTCCAAATCATATGCCCTCTTCAATCAATTTACTTGTGCAGAATCAGCAGACACCAAACAGTGCTATTTTAACAGGAAACAAGGCCAATGAAGAGGAGGAGGAGGAAATAATAGATGATGATGATGACACTATTAGCTCCAGTCCTGACTCGGCCGTCAGTAATACATCTTTGGTCCCACAGGCTGATACCTCCCAAAATACCAGTTTTGATGGATCATTAATACAGAAGATGCAGATTCCTACACTTCTTCAAGAACCACTTTCCAATTCCTTAAAAATTTCAGATATAATTACTAGAAATACTAATGATCCAGGCGTAGGATCAAAACATCTAATGGAGGGTCAGAAGATCATTACTTTAGATACAGCTACTGAAATTGAAGGCTTATCGACTGGTTGCAAGGTTTATGCAAATATCGGTGAAGATACTTATGATATAGTGATCCCTGTCAAAGATGACCCTGATGAAGGGGAGGCCAGACTTGAGAATGAAATACCAAAAACGTCTGGCAGCGAGATGGCAAACAAACGTATGAAAGTAAAACATGATGATCACTATGAGTTAATAGTAGATGGAAGGGTCTATTATATCTGTATTGTATGCAAAAGGTCATATGTCTGTCTGACAAGCTTGCGGAGACATTTTAACATTCATTCTTGGGAGAAGAAGTATCCGTGCCGTTACTGTGAGAAGGTATTTCCTCTTGCAGAATATCGCACAAAGCATGAAATTCATCACACAGGGGAGCGAAGGTATCAGTGTTTGGCCTGTGGCAAATCTTTCATCAACTATCAGTTTATGTCTTCACATATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTTATCGTTTACATCCATGCAGGTCTTTACAAATCAGACAATATGCATATCTTTCCGATAGATCAAGCACTATTCCTGCAATGAAGGATGATGGTATTGGGTATAAGGTTGACACTGGAAAAGAACCTCCAGTAGGGACCACTACATCTACTCAGAACAAGCCAATGACCTGGGAAGATATTTTTATTCAGCAGGAAAATGATTCAATTTTTAAACAAAATGTAACAGATGGCAGTACTGAGTTTGAATTTATAATACCAGAGTCTTACTAA
ORF Protein Sequence MESRKLISATDIQYSGSLLNSLNEQRGHGLFCDVTVIVEDRKFRAHKNILSASSTYFHQLFSVAGQVVELSFIRAEIFAEILNYIYSSKIVRVRSDLLDELIKSGQLLGVKFIAELGVPLSQVKSISGTAQDGNTEPLPPDSGDKNLVIQKSKDEAQDNGATIMPIITESFSLSAEDYEMKKIIVTDSDDDDDDVIFCSEILPTKETLPSNNTVAQVQSNPGPVAISDVAPSASNNSPPLTNITPTQKLPTPVNQATLSQTQGSEKLLVSSAPTHLTPNIILLNQTPLSTPPNVSSSLPNHMPSSINLLVQNQQTPNSAILTGNKANEEEEEEIIDDDDDTISSSPDSAVSNTSLVPQADTSQNTSFDGSLIQKMQIPTLLQEPLSNSLKISDIITRNTNDPGVGSKHLMEGQKIITLDTATEIEGLSTGCKVYANIGEDTYDIVIPVKDDPDEGEARLENEIPKTSGSEMANKRMKVKHDDHYELIVDGRVYYICIVCKRSYVCLTSLRRHFNIHSWEKKYPCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSFINYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-MP2391-Ab Anti-KAISO/ ZBTB33/ ZNF-kaiso monoclonal antibody
    Target Antigen GM-Tg-g-MP2391-Ag ZBTB33 VLP (virus-like particle)
    ORF Viral Vector pGMLP001312 Human ZBTB33 Lentivirus plasmid
    ORF Viral Vector pGMAD000086 Human ZBTB33 Adenovirus plasmid
    ORF Viral Vector pGMPC001859 Human ZBTB33 Mammalian (Non-Viral Vector) plasmid
    ORF Viral Vector vGMLP001312 Human ZBTB33 Lentivirus particle
    ORF Viral Vector vGMAD000086 Human ZBTB33 Adenovirus particle


    Target information

    Target ID GM-MP2391
    Target Name ZBTB33
    Gene ID 10009, 56805, 695801, 501506, 101086378, 100685062, 784871, 100062002
    Gene Symbol and Synonyms E130014G12Rik,Kaiso,RGD1566309,ZBTB33,ZNF-kaiso,ZNF348
    Uniprot Accession Q86T24
    Uniprot Entry Name KAISO_HUMAN
    Protein Sub-location Transmembrane Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000177485
    Target Classification Not Available

    This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010]



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