Diagnostic anti-swine influenza A virus antibodies pairs and antigens for animal health (animal infectious disease ) testing in ELISA test,competitive ELISA test, blocking ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), and immunonephelometry

Definition of the disease: Swine Influenza (swine flu) is a respiratory disease of pigs caused by type A influenza virus that regularly causes outbreaks of influenza in pigs. Swine flu viruses can cause high levels of illness in pig herds, but cause few deaths in pigs. Swine influenza viruses can circulate among swine throughout the year, but most outbreaks occur during the late fall and winter months similar to outbreaks of seasonal influenza in humans. The main swine flu viruses circulating in U.S. pigs in recent years are: swine triple reassortant (tr) H1N1, trH3N2 and trH1N2. With the exception of the 2009 H1N1 virus, influenza viruses that circulate in swine are very different from influenza viruses that commonly circulate in people.

Genemedi produces core animal health diagnostic ingredients-validated antibodies pairs Mouse anti-swine influenza A virus monoclonal antibody and swine influenza A virus antigens for rapid test kit of animal infectious disease with swine influenza A virus to evaluate the animal health of Pig.

The paired antibodies are both monoclonal antibody(mab).

All the antibodies and antiges of animal disease test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibody can act as a capture antibody and detection antibody. Antigens are validated as positive control materials.

Order information

Catalog No. (1~4, 4 antibodies in pairs)	Size	Price(In USD)	Qty (Quantity)	Sum(In USD)
GMP-AD-Pig-17Ab-1	Size:1mg	3090
GMP-AD-Pig-17Ab-1	Size:10mg	21935
GMP-AD-Pig-17Ab-1	Size:100mg	148000
GMP-AD-Pig-17Ab-2	Size:1mg	3090
GMP-AD-Pig-17Ab-2	Size:10mg	21935
GMP-AD-Pig-17Ab-2	Size:100mg	148000
GMP-AD-Pig-17Ab-3	Size:1mg	3090
GMP-AD-Pig-17Ab-3	Size:10mg	21935
GMP-AD-Pig-17Ab-3	Size:100mg	148000
GMP-AD-Pig-17Ab-4	Size:1mg	3090
GMP-AD-Pig-17Ab-4	Size:10mg	21935
GMP-AD-Pig-17Ab-4	Size:100mg	148000
GMP-AD-Pig-17Ag-1	Size:1mg	3090
GMP-AD-Pig-17Ag-1	Size:10mg	21935
GMP-AD-Pig-17Ag-1	Size:100mg	148000
GMP-AD-Pig-17Ag-2	Size:1mg	3090
GMP-AD-Pig-17Ag-2	Size:10mg	21935
GMP-AD-Pig-17Ag-2	Size:100mg	148000
			Shipping Cost:	760.00
			Total:

Description

GMP-AD-Pig-17Ab, GMP-AD-Pig-17Ag

Cat No.	GMP-AD-Pig-17Ab
Antigens	swine influenza A virus
Antibody	Mouse anti-swine influenza A virus monoclonal antibody
Resource (expression host)	hybridoma
Specics/Isotypes	Mouse IgG
Bioactivity validation	Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays in swine influenza A virus level test and Pig-diagnositcs.
Antigen description	The porcine epidemic diarrhea virus (PEDV) causes an acute and highly contagious enteric disease characterized by severe enteritis, vomiting, watery diarrhea, and a high mortality rate in seronegative neonatal piglets. In the last few years, PED had a large economic impact on the swine industries in Asia and the US, and in 2014, the PEDV also re-emerged in Europe. Two main PEDV variants circulate worldwide but only the S INDEL variant, considered a mild strain, is spreading in Europe. To gain insights into the pathogenicity of this variant, its viral load and temporal shedding pattern were evaluated in piglets from infected farms. Quantitative real-time PCR (qPCR) targeting the spike gene, was validated according to the minimum information for quantitative real-time PCR experiments guidelines. The qPCR was applied to longitudinal studies conducted in four swine farms naturally infected with the PEDV S INDEL variant. Clinical data, fecal swabs, and blood samples were collected from 103 piglets at 15–30-day intervals for 2–5 months. On all four farms, diarrhea was observed in sows during gestation and in farrowing units, and the mortality rates of piglets were 18, 25, 30, and 35%. Different clinical pictures (0-50% of diarrhea positivity), viral titer levels (mean 5.3-7.2 log10 genome copies/mL), and antibody conditions (30-80% of positivity) were registered among sows on the four farms. The percentage of qPCR positive piglets varied greatly from the beginning (63–100%) to the end (0%) of the infection course. Clinical signs were present in 96% of the qPCR positive animals. Viral loads ranged from 8.5 log10 to 4 log10 genome copies/mL in suckling pigs at 3–6 days of age and were not statistically different among farms, despite the different patterns observed in sows. After 2–3 weeks, only a few piglets still showed detectable viral levels and clinical signs, and they developed antibody responses. Moreover, co-infections with other pathogens and biosecurity procedures limiting the circulation of the virus could have influenced the severity of PED infection. QPCR and clinical data were useful in understanding the dynamics of PEDV infections and, therefore, in implementing appropriate control measures.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, Lateral flow immunoassay (LFIA), and other immunoassays;
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-AD-Pig-17Ag
Antigens	swine influenza A virus
Resource (expression host)	E.coli
Specics/Isotypes	swine influenza A virus
Bioactivity validation	Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays in swine influenza A virus level test and Pig-diagnositcs.
Tag	His
Antigen description	The porcine epidemic diarrhea virus (PEDV) causes an acute and highly contagious enteric disease characterized by severe enteritis, vomiting, watery diarrhea, and a high mortality rate in seronegative neonatal piglets. In the last few years, PED had a large economic impact on the swine industries in Asia and the US, and in 2014, the PEDV also re-emerged in Europe. Two main PEDV variants circulate worldwide but only the S INDEL variant, considered a mild strain, is spreading in Europe. To gain insights into the pathogenicity of this variant, its viral load and temporal shedding pattern were evaluated in piglets from infected farms. Quantitative real-time PCR (qPCR) targeting the spike gene, was validated according to the minimum information for quantitative real-time PCR experiments guidelines. The qPCR was applied to longitudinal studies conducted in four swine farms naturally infected with the PEDV S INDEL variant. Clinical data, fecal swabs, and blood samples were collected from 103 piglets at 15–30-day intervals for 2–5 months. On all four farms, diarrhea was observed in sows during gestation and in farrowing units, and the mortality rates of piglets were 18, 25, 30, and 35%. Different clinical pictures (0-50% of diarrhea positivity), viral titer levels (mean 5.3-7.2 log10 genome copies/mL), and antibody conditions (30-80% of positivity) were registered among sows on the four farms. The percentage of qPCR positive piglets varied greatly from the beginning (63–100%) to the end (0%) of the infection course. Clinical signs were present in 96% of the qPCR positive animals. Viral loads ranged from 8.5 log10 to 4 log10 genome copies/mL in suckling pigs at 3–6 days of age and were not statistically different among farms, despite the different patterns observed in sows. After 2–3 weeks, only a few piglets still showed detectable viral levels and clinical signs, and they developed antibody responses. Moreover, co-infections with other pathogens and biosecurity procedures limiting the circulation of the virus could have influenced the severity of PED infection. QPCR and clinical data were useful in understanding the dynamics of PEDV infections and, therefore, in implementing appropriate control measures.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, Lateral flow immunoassay (LFIA), and other immunoassays;
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -145℃ under sterile conditions. Avoid repeated freeze-thaw cycles.