Phospho-tau231 (p-tau231) antibody and antigen (Recombinant protein)
Diagnostic Phospho-tau231 (p-tau231) antibodies (anti-Phospho-tau231, anti-p-tau231) and antigens (recombinant Phospho-tau231 (p-tau231) protein) for Neurodegenerative diseases p-tau231 detection in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT.
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Product information
Catalog No. | Description | US $ Price (per mg) |
---|---|---|
GMP-h-p-tau231-Ag01 | Phospho-tau231 (p-tau231) antigen | 1823 |
GMP-h-p-tau231-Ag02 | Recombinant human Phospho-tau231 (p-tau231) Protein (full length) | 28000 |
GMP-h-p-tau231-Ab01 | Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) | 1953 |
GMP-h-p-tau231-Ab02 | Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) | 1953 |
GMP-h-p-tau231-Ab03 | Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) | 1953 |
GMP-h-p-tau231-Ab04 | Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) | 1953 |
Size: 1mg | 10mg | 100mg
Product Description
Cat No. of Products | GMP-h-p-tau231-Ag01 |
Product Name | Phospho-tau231 (p-tau231) antigen |
Target/Biomarker | Phospho-tau231 (p-tau231) |
Alias of Target/Biomarker | DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40 |
Expression platform | synthesized |
Isotypes | NA |
Bioactivity validation | Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation. |
Products description | Phospho-tau231 (p-tau231) antigen was expressed in synthesized expression system and is expressed with 6 HIS tag at the C-terminus. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | positive control, ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Cat No. of Products | GMP-h-p-tau231-Ag02 |
Product Name | Recombinant human Phospho-tau231 (p-tau231) Protein (full length) |
Target/Biomarker | Phospho-tau231 (p-tau231) |
Alias of Target/Biomarker | DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40 |
Expression platform | mammalian |
Isotypes | Recombinant Antigen |
Bioactivity validation | Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation. |
Products description | Recombinant human Phospho-tau231 (p-tau231) Protein (full length) was expressed in mammalian expression system and is expressed with 6 HIS tag at the C-terminus. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | positive control, ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Cat No. of Products | GMP-h-p-tau231-Ab01, GMP-h-p-tau231-Ab02 |
Product Name | Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) |
Target/Biomarker | Phospho-tau231 (p-tau231) |
Alias of Target/Biomarker | DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40 |
Expression platform | CHO |
Isotypes | Mouse IgG |
Bioactivity validation | Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation. |
Tag | mFc |
Products description | Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) was expressed in CHO expression system and is expressed with 6 HIS tag at the C-terminus. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT |
Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Cat No. of Products | GMP-h-p-tau231-Ab03, GMP-h-p-tau231-Ab04 |
Product Name | Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) |
Target/Biomarker | Phospho-tau231 (p-tau231) |
Alias of Target/Biomarker | DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40 |
Expression platform | CHO |
Isotypes | Human lgG1 |
Bioactivity validation | Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation. |
Tag | hFc |
Products description | Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) was expressed in CHO expression system and is expressed with 6 HIS tag at the C-terminus. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT |
Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Reference
Validation Data
Good performance of GeneMedi's p-tau/tau antibodies in ELISA Validation
1. GeneMedi's three phosphorylated tau protein antibodies (p-tau181, p-tau217, and p-tau231) demonstrate outstanding sensitivity and exceptional specificity with their corresponding phosphorylated forms in direct ELISA assays.
GeneMedi's p-tau antibodies and antigens demonstrate excellent binding affinity: GMP-h-p-tau181-Ab01 effectively binds with GMP-h-p-tau181-Ag01, GMP-h-p-tau217-Ab01 binds well with GMP-h-p-tau217-Ag01, and GMP-h-p-tau231-Ab01 shows strong binding with GMP-h-p-tau231-Ag01. These results indicate that GeneMedi's p-tau antibodies and antigens possess outstanding affinity.
Product used as below:
Cat NO. | Description |
GMP-h-p-tau181-Ab01 | Anti-Phospho-tau181 (p-tau181) mouse monoclonal antibody (mAb) |
GMP-h-p-tau181-Ag01 | Phospho-tau181 (p-tau181) antigen (peptide) |
GMP-h-p-tau217-Ab01 | Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb) |
GMP-h-p-tau217-Ag01 | Phospho-tau217 (p-tau217) antigen (peptide) |
GMP-h-tau231-Ab01 | Anti-Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) |
GMP-h-p-tau231-Ag01 | Phospho-tau231 (p-tau231) antigen (peptide) |
Fig 1. Validation of GeneMedi's Anti-human p-tau181/p-tau217/p-tau231 antibody in direct ELISA.
A. GeneMedi's GMP-h-p-tau181-Ab01 (Anti-human p-tau181 antibody) is validated to detect the GMP-h-p-tau181-Ag01 (p-tau181 antigen (peptide)) in ELISA, EC50 = 25.26 ng/ml.
B. GeneMedi's GMP-h-p-tau217-Ab01 (Anti-human p-tau217 antibody) is validated to detect the GMP-h-p-tau217-Ag01 (p-tau217 antigen (peptide)) in ELISA, EC50 = 25.85 ng/ml.
C.
GeneMedi's GMP-h-p-tau231-Ab01 (Anti-human p-tau231 antibody) is validated to detect the GMP-h-p-tau231-Ag01 (p-tau231 antigen (peptide)) in ELISA, EC50 = 64.34 ng/ml.
The total tau antibody (GMP-h-TauAb01) does not interact with the p-tau181 (GMP-h-p-tau181-Ag01), p-tau217 (GMP-h-p-tau217-Ag01), and p-tau231 (GMP-h-p-tau231-Ag01) peptide antigen. (Data not shown)
2. In direct ELISA assays, GeneMedi's phosphorylated tau217 antibody demonstrates excellent sensitivity and precision for both full-length and peptide forms of the phosphorylated tau217 antigen. However, its ability to detect other phosphorylated antigens and total tau antigen is limited, indicating its highly specificity.
GeneMedi's GMP-h-p-tau217-Ab01 antibody demonstrates excellent binding ability with the full-length antigen GMP-h-p-tau217-Ag02 as well as the peptide GMP-h-p-tau217-Ag01. However, when interacting with the antigens GMP-h-p-tau181-Ag01, GMP-h-p-tau231-Ag01, and GMP-h-Tau-Ag01, the affinity is relatively weaker, as reflected by significant differences in EC50 values. These results indicate that GeneMedi's p-tau 217 antibody exhibits high affinity and specificity. Product used as below:
Cat NO. | Description |
GMP-h-p-tau181-Ag01 | Phospho-tau181(p-tau181) antigen (peptide) |
GMP-h-p-tau217-Ag01 | Phospho-tau217 (p-tau217) antigen (peptide) |
GMP-h-p-tau231-Ag01 | Phospho-tau231 (p-tau231) antigen (peptide) |
GMP-h-p-tau217-Ag02 | Phospho-tau217 (p-tau217) antigen (full length) |
GMP-h-Tau-Ag01 | Recombinant human MAPT/Tau/MAPTL/MTBT1 Protein |
GMP-h-p-tau217-Ab01 | Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb) |
Fig 2. Direct ELISA Binding Curves of Phosphorylated Tau217 Antibody with Various Antigens.
This graph displays the binding efficiency of the phosphorylated tau217 antibody with five different antigens in an ELISA setup. The x-axis represents the antibody concentration in a logarithmic scale (μg/ml), and the y-axis indicates the corrected absorbance, reflecting the binding efficiency between the antibody and antigens. The data illustrate that GMP-h-p-tau217-Ab01 exhibits high detection efficiency for GMP-h-p-tau217-Ag01 (peptide) with an EC50 of 4.326 ng/ml. For GMP-h-p-tau217-Ag02 (full length), the antibody demonstrates exceptionally high sensitivity, with an EC50 of only 6.943 ng/ml. Additionally, this antibody effectively detects GMP-h-p-tau181-Ag01 (peptide), GMP-h-p-tau231-Ag01 (peptide) and GMP-h-tau-Ag01 (full length) with EC50 values of 27320 ng/ml, 3563 ng/ml and 1843 ng/ml.
3. In sandwich ELISA assays, GeneMedi's p-tau217 antibody and tau antibody pair can detect the full-length phosphorylated 217 protein but are unable to detect phosphorylated 217 peptides or non-phosphorylated proteins.
Cat NO. | Description |
GMP-h-p-tau217 Ag01 | Phospho-tau217 (p-tau217) antigen (peptide) |
GMP-h-p-tau217 Ag02 | Phospho-tau217 (p-tau217) antigen (full length) |
GMP-h-p-tau217 Ab01 | Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb) |
GMP-h-p-tau217 Ab02 | Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb) |
GMP-h-tau-Ag01 | Recombinant human MAPT/Tau/MA PTL/MTBT1 Protein |
GMP-h-tauAb01 | A nti-human MAPT/Tau/MA PTL/MTBT1 mouse monoclonal antibody (mAb) |
GMP-h-tau-Ab02 | Anti-human MAPTITau/MA PTL/MTBT1 mouse monoclonal antibody (mAb) |
GMP-h-tau-Ab03 | Anti-human MAPTITau/MA PTL/MTBT1 mouse monoclonal antibody (mAb) |
GMP-h-p-tau217-Ab01 is used as the capture antibody. When GMP-h-Tau-Ab03 is used as the detection antibody, the EC50 for detecting GMP-h-p-tau217-Ag02 is lower than GMP-h-Tau-Ab01, indicating GMP-h-Tau-Ab03 is more suitable as a detection antibody.
However, when detecting phosphorylated 217 peptide GMP-h-p-tau217-Ag01 or non-phosphorylated protein GMP-h-tau-Ag01, the EC50 remains high regardless of whether GMP-h-Tau-Ab03 or GMP-h-Tau-Ab01 is used, suggesting no binding for detecting peptide GMP-h-p-tau217-Ag01 or non-phosphorylated protein GMP-h-tau-Ag01 under these conditions.
Fig3. Sandwich ELISA Analysis of Full-Length Phosphorylated Tau 217 Antigen with Corresponding Phosphorylated Tau 217 Antibody and Total Tau Antibody.
A.This figure illustrates the detection efficacy of two different antibody combinations in Sandwich ELISA experiments targeting the GMP-h-p-tau217-Ag02 antigen (full length). The x-axis represents the antigen concentration displayed on a logarithmic scale (μg/ml), while the y-axis shows the corrected absorbance. The results indicate that the combination of GMP-h-p-tau217-Ab01 as the coating antibody and GMP-h-Tau-Ab01 as the detection antibody yields an EC50 of 76.55 ng/ml. Conversely, using GMP-h-p-tau217-Ab01 as the coating antibody and GMP-h-Tau-Ab03 as the detection antibody results in an EC50 of 39.02 ng/ml, demonstrating higher detection sensitivity at lower concentrations.
B.This figure demonstrates the detection results of two different detection antibodies (GMP-h-tau-Ab01 and GMP-h-tau-Ab03) against the GMP-h-tau-Ag01 antigen (full length) in an ELISA assay, using GMP-h-p-tau217-Ab01 as the coating antibody. The x-axis represents antigen concentration, displayed on a logarithmic scale (μg/ml); the y-axis shows corrected absorbance. The results reveal that when using GMP-h-tau-Ab03 as the detection antibody, the EC50 value reaches 20030 ng/ml, indicating a lower sensitivity to the antigen; whereas, when using GMP-h-tau-Ab01 as the detection antibody, the EC50 value soars to 4352000 ng/ml, demonstrating poor efficacy in detecting the antigen.
C. This figure illustrates the detection performance of four different antibody combinations in an ELISA assay targeting the GMP-h-p-tau217-Ag01 antigen (peptide). The x-axis represents antigen concentration, displayed on a logarithmic scale (μg/ml), while the y-axis shows the corrected absorbance. The curves in the figure show that all antibody combinations maintain relatively consistent absorbance changes across a range of low to high concentrations, indicating these results also reveal that the sensitivity of these four antibody combinations in detecting the GMP-h-p-tau217-Ag01 (peptide) antigen is relatively low.
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Associated products
- Recommend Pair Antibody and Antigen
- Associated products
Pair Antibody and Antigen | Products detail | Cat.No | Product Name |
P-tau217 | 1. Antigen | 1. GMP-h-p-tau217-Ag01 | Phospho-tau217 (p-tau217) antigen |
2. Capture antibody | 2. GMP-h-p-tau217-Ab01 | Anti-human p-tau217 mouse monoclonal antibody(mAb) | |
3. Detector antibody | 3. GMP-h-Tau-Ab03 | Recombinant human Tau Protein | |
P-tau181 | 1. Antigen | 1. GMP-h-p-tau181-Ag01 | Phospho-tau181 (p-tau181) antigen |
2. Capture antibody | 2. GMP-h-p-tau181-Ab01 | Anti-human p-tau181 mouse monoclonal antibody(mAb) | |
3. Detector antibody | 3. GMP-h-Tau-Ab03 | Recombinant human Tau Protein | |
P-tau231 | 1. Antigen | 1. GMP-h-p-tau231-Ag01 | Phospho-tau231 (p-tau231) antigen |
2. Capture antibody | 2. GMP-h-p-tau231-Ab01 | Anti-human p-tau231 mouse monoclonal antibody(mAb) | |
3. Detector antibody | 3. GMP-h-Tau-Ab03 | Recombinant human Tau Protein |
Catalog No. | Products Name | Biomarker | Fc/Tag | Products Information |
GMP-h-p-tau202/205-Ab | Anti-human p-tau202 and 205 monoclonal antibody(mAb) | Phospho-tau202 and 205 (p-tau202 and 205) | hFc/mFc | Details |
GMP-h-p-tau212/214-Ab | Anti-human p-tau212 and 214 monoclonal antibody(mAb) | Phospho-tau212 and 214 (p-tau212 and 214) | hFc/mFc | Details |
GMP-h-p-tau413-Ab | Anti-human p-tau413 monoclonal antibody(mAb) | Phospho-tau413 (p-tau413) | hFc/mFc | Details |
GMP-h-p-tau422-Ab | Anti-human p-tau422 monoclonal antibody(mAb) | Phospho-tau422 (p-tau422) | hFc/mFc | Details |
GMP-h-Tau-Ab | Anti-human Tau monoclonal antibody(mAb) | tau proteins (Tau) | hFc/mFc | Details |
Target/Biomarker information
Alzheimer Disease (AD) Recent publications and meeting presentations bolster the evidence that upticks in p-tau181, p-tau217, and p-tau231 occur soon after Aβ begins to accumulate in the brain. That’s years before neurofibrillary tangles can be detected by PET, and decades before symptoms. Researchers predict these markers, which also distinguish AD from other forms of dementia, will lead to more robust tests than those based on Aβ, because of their larger dynamic range—phosphorylated fragments of tau climb by as much as 10-fold in the blood as AD progresses, whereas the Aβ42/40 ratio falls by about 15 percent at most.
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