Phospho-tau231 (p-tau231) antibody and antigen (Recombinant protein)

Diagnostic Phospho-tau231 (p-tau231) antibodies (anti-Phospho-tau231, anti-p-tau231) and antigens (recombinant Phospho-tau231 (p-tau231) protein) for Neurodegenerative diseases p-tau231 detection in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT.

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Product information

Catalog No. Description US $ Price (per mg)
GMP-h-p-tau231-Ag01 Phospho-tau231 (p-tau231) antigen 1823
GMP-h-p-tau231-Ag02 Recombinant human Phospho-tau231 (p-tau231) Protein (full length) 28000
GMP-h-p-tau231-Ab01 Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) 1953
GMP-h-p-tau231-Ab02 Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) 1953
GMP-h-p-tau231-Ab03 Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) 1953
GMP-h-p-tau231-Ab04 Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) 1953

Size: 1mg | 10mg | 100mg



Product Description

Cat No. of Products GMP-h-p-tau231-Ag01
Product Name Phospho-tau231 (p-tau231) antigen
Target/Biomarker Phospho-tau231 (p-tau231)
Alias of Target/Biomarker DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
Expression platform synthesized
Isotypes NA
Bioactivity validation Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation.
Products description Phospho-tau231 (p-tau231) antigen was expressed in synthesized expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application positive control, ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products GMP-h-p-tau231-Ag02
Product Name Recombinant human Phospho-tau231 (p-tau231) Protein (full length)
Target/Biomarker Phospho-tau231 (p-tau231)
Alias of Target/Biomarker DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
Expression platform mammalian
Isotypes Recombinant Antigen
Bioactivity validation Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation.
Products description Recombinant human Phospho-tau231 (p-tau231) Protein (full length) was expressed in mammalian expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application positive control, ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products GMP-h-p-tau231-Ab01, GMP-h-p-tau231-Ab02
Product Name Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb)
Target/Biomarker Phospho-tau231 (p-tau231)
Alias of Target/Biomarker DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation.
Tag mFc
Products description Anti-human Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb) was expressed in CHO expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products GMP-h-p-tau231-Ab03, GMP-h-p-tau231-Ab04
Product Name Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb)
Target/Biomarker Phospho-tau231 (p-tau231)
Alias of Target/Biomarker DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
Expression platform CHO
Isotypes Human lgG1
Bioactivity validation Phospho-tau231 (p-tau231) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in p-tau231 level test of Neurodegenerative diseases (Alzheimer Disease (AD)) and related syndrome evaluation.
Tag hFc
Products description Anti-human Phospho-tau231 (p-tau231) human monoclonal antibody (mAb) was expressed in CHO expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference




    Validation Data


    Good performance of GeneMedi's p-tau/tau antibodies in ELISA Validation

    1. GeneMedi's three phosphorylated tau protein antibodies (p-tau181, p-tau217, and p-tau231) demonstrate outstanding sensitivity and exceptional specificity with their corresponding phosphorylated forms in direct ELISA assays.

    Tau ELISA Validation
    Click to Zoom

    GeneMedi's p-tau antibodies and antigens demonstrate excellent binding affinity: GMP-h-p-tau181-Ab01 effectively binds with GMP-h-p-tau181-Ag01, GMP-h-p-tau217-Ab01 binds well with GMP-h-p-tau217-Ag01, and GMP-h-p-tau231-Ab01 shows strong binding with GMP-h-p-tau231-Ag01. These results indicate that GeneMedi's p-tau antibodies and antigens possess outstanding affinity.

    Product used as below:

    Cat NO. Description
    GMP-h-p-tau181-Ab01 Anti-Phospho-tau181 (p-tau181) mouse monoclonal antibody (mAb)
    GMP-h-p-tau181-Ag01 Phospho-tau181 (p-tau181) antigen (peptide)
    GMP-h-p-tau217-Ab01 Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)
    GMP-h-p-tau217-Ag01 Phospho-tau217 (p-tau217) antigen (peptide)
    GMP-h-tau231-Ab01 Anti-Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb)
    GMP-h-p-tau231-Ag01 Phospho-tau231 (p-tau231) antigen (peptide)

    Fig 1. Validation of GeneMedi's Anti-human p-tau181/p-tau217/p-tau231 antibody in direct ELISA.

    A. GeneMedi's GMP-h-p-tau181-Ab01 (Anti-human p-tau181 antibody) is validated to detect the GMP-h-p-tau181-Ag01 (p-tau181 antigen (peptide)) in ELISA, EC50 = 25.26 ng/ml.
    B. GeneMedi's GMP-h-p-tau217-Ab01 (Anti-human p-tau217 antibody) is validated to detect the GMP-h-p-tau217-Ag01 (p-tau217 antigen (peptide)) in ELISA, EC50 = 25.85 ng/ml.
    C. GeneMedi's GMP-h-p-tau231-Ab01 (Anti-human p-tau231 antibody) is validated to detect the GMP-h-p-tau231-Ag01 (p-tau231 antigen (peptide)) in ELISA, EC50 = 64.34 ng/ml.
    The total tau antibody (GMP-h-TauAb01) does not interact with the p-tau181 (GMP-h-p-tau181-Ag01), p-tau217 (GMP-h-p-tau217-Ag01), and p-tau231 (GMP-h-p-tau231-Ag01) peptide antigen. (Data not shown)

    2. In direct ELISA assays, GeneMedi's phosphorylated tau217 antibody demonstrates excellent sensitivity and precision for both full-length and peptide forms of the phosphorylated tau217 antigen. However, its ability to detect other phosphorylated antigens and total tau antigen is limited, indicating its highly specificity.

    Tau ELISA Validation
    Click to Zoom

    GeneMedi's GMP-h-p-tau217-Ab01 antibody demonstrates excellent binding ability with the full-length antigen GMP-h-p-tau217-Ag02 as well as the peptide GMP-h-p-tau217-Ag01. However, when interacting with the antigens GMP-h-p-tau181-Ag01, GMP-h-p-tau231-Ag01, and GMP-h-Tau-Ag01, the affinity is relatively weaker, as reflected by significant differences in EC50 values. These results indicate that GeneMedi's p-tau 217 antibody exhibits high affinity and specificity. Product used as below:

    Cat NO. Description
    GMP-h-p-tau181-Ag01 Phospho-tau181(p-tau181) antigen (peptide)
    GMP-h-p-tau217-Ag01 Phospho-tau217 (p-tau217) antigen (peptide)
    GMP-h-p-tau231-Ag01 Phospho-tau231 (p-tau231) antigen (peptide)
    GMP-h-p-tau217-Ag02 Phospho-tau217 (p-tau217) antigen (full length)
    GMP-h-Tau-Ag01 Recombinant human MAPT/Tau/MAPTL/MTBT1 Protein
    GMP-h-p-tau217-Ab01 Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)

    Fig 2. Direct ELISA Binding Curves of Phosphorylated Tau217 Antibody with Various Antigens.

    This graph displays the binding efficiency of the phosphorylated tau217 antibody with five different antigens in an ELISA setup. The x-axis represents the antibody concentration in a logarithmic scale (μg/ml), and the y-axis indicates the corrected absorbance, reflecting the binding efficiency between the antibody and antigens. The data illustrate that GMP-h-p-tau217-Ab01 exhibits high detection efficiency for GMP-h-p-tau217-Ag01 (peptide) with an EC50 of 4.326 ng/ml. For GMP-h-p-tau217-Ag02 (full length), the antibody demonstrates exceptionally high sensitivity, with an EC50 of only 6.943 ng/ml. Additionally, this antibody effectively detects GMP-h-p-tau181-Ag01 (peptide), GMP-h-p-tau231-Ag01 (peptide) and GMP-h-tau-Ag01 (full length) with EC50 values of 27320 ng/ml, 3563 ng/ml and 1843 ng/ml.

    3. In sandwich ELISA assays, GeneMedi's p-tau217 antibody and tau antibody pair can detect the full-length phosphorylated 217 protein but are unable to detect phosphorylated 217 peptides or non-phosphorylated proteins.

    Tau ELISA Validation
    Click to Zoom

    Cat NO. Description
    GMP-h-p-tau217 Ag01 Phospho-tau217 (p-tau217) antigen (peptide)
    GMP-h-p-tau217 Ag02 Phospho-tau217 (p-tau217) antigen (full length)
    GMP-h-p-tau217 Ab01 Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)
    GMP-h-p-tau217 Ab02 Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)
    GMP-h-tau-Ag01 Recombinant human MAPT/Tau/MA PTL/MTBT1 Protein
    GMP-h-tauAb01 A nti-human MAPT/Tau/MA PTL/MTBT1 mouse monoclonal antibody (mAb)
    GMP-h-tau-Ab02 Anti-human MAPTITau/MA PTL/MTBT1 mouse monoclonal antibody (mAb)
    GMP-h-tau-Ab03 Anti-human MAPTITau/MA PTL/MTBT1 mouse monoclonal antibody (mAb)

    GMP-h-p-tau217-Ab01 is used as the capture antibody. When GMP-h-Tau-Ab03 is used as the detection antibody, the EC50 for detecting GMP-h-p-tau217-Ag02 is lower than GMP-h-Tau-Ab01, indicating GMP-h-Tau-Ab03 is more suitable as a detection antibody.
    However, when detecting phosphorylated 217 peptide GMP-h-p-tau217-Ag01 or non-phosphorylated protein GMP-h-tau-Ag01, the EC50 remains high regardless of whether GMP-h-Tau-Ab03 or GMP-h-Tau-Ab01 is used, suggesting no binding for detecting peptide GMP-h-p-tau217-Ag01 or non-phosphorylated protein GMP-h-tau-Ag01 under these conditions.

    Fig3. Sandwich ELISA Analysis of Full-Length Phosphorylated Tau 217 Antigen with Corresponding Phosphorylated Tau 217 Antibody and Total Tau Antibody.

    A.This figure illustrates the detection efficacy of two different antibody combinations in Sandwich ELISA experiments targeting the GMP-h-p-tau217-Ag02 antigen (full length). The x-axis represents the antigen concentration displayed on a logarithmic scale (μg/ml), while the y-axis shows the corrected absorbance. The results indicate that the combination of GMP-h-p-tau217-Ab01 as the coating antibody and GMP-h-Tau-Ab01 as the detection antibody yields an EC50 of 76.55 ng/ml. Conversely, using GMP-h-p-tau217-Ab01 as the coating antibody and GMP-h-Tau-Ab03 as the detection antibody results in an EC50 of 39.02 ng/ml, demonstrating higher detection sensitivity at lower concentrations.
    B.This figure demonstrates the detection results of two different detection antibodies (GMP-h-tau-Ab01 and GMP-h-tau-Ab03) against the GMP-h-tau-Ag01 antigen (full length) in an ELISA assay, using GMP-h-p-tau217-Ab01 as the coating antibody. The x-axis represents antigen concentration, displayed on a logarithmic scale (μg/ml); the y-axis shows corrected absorbance. The results reveal that when using GMP-h-tau-Ab03 as the detection antibody, the EC50 value reaches 20030 ng/ml, indicating a lower sensitivity to the antigen; whereas, when using GMP-h-tau-Ab01 as the detection antibody, the EC50 value soars to 4352000 ng/ml, demonstrating poor efficacy in detecting the antigen.
    C. This figure illustrates the detection performance of four different antibody combinations in an ELISA assay targeting the GMP-h-p-tau217-Ag01 antigen (peptide). The x-axis represents antigen concentration, displayed on a logarithmic scale (μg/ml), while the y-axis shows the corrected absorbance. The curves in the figure show that all antibody combinations maintain relatively consistent absorbance changes across a range of low to high concentrations, indicating these results also reveal that the sensitivity of these four antibody combinations in detecting the GMP-h-p-tau217-Ag01 (peptide) antigen is relatively low.

    Click to get more Data / Case study about the product.



    Associated products


    • Recommend Pair Antibody and Antigen
    • Associated products
    Pair Antibody and Antigen Products detail Cat.No Product Name
    P-tau217 1. Antigen 1. GMP-h-p-tau217-Ag01 Phospho-tau217 (p-tau217) antigen
    2. Capture antibody 2. GMP-h-p-tau217-Ab01 Anti-human p-tau217 mouse monoclonal antibody(mAb)
    3. Detector antibody 3. GMP-h-Tau-Ab03 Recombinant human Tau Protein
    P-tau181 1. Antigen 1. GMP-h-p-tau181-Ag01 Phospho-tau181 (p-tau181) antigen
    2. Capture antibody 2. GMP-h-p-tau181-Ab01 Anti-human p-tau181 mouse monoclonal antibody(mAb)
    3. Detector antibody 3. GMP-h-Tau-Ab03 Recombinant human Tau Protein
    P-tau231 1. Antigen 1. GMP-h-p-tau231-Ag01 Phospho-tau231 (p-tau231) antigen
    2. Capture antibody 2. GMP-h-p-tau231-Ab01 Anti-human p-tau231 mouse monoclonal antibody(mAb)
    3. Detector antibody 3. GMP-h-Tau-Ab03 Recombinant human Tau Protein


    Catalog No. Products Name Biomarker Fc/Tag Products Information
    GMP-h-p-tau202/205-Ab Anti-human p-tau202 and 205 monoclonal antibody(mAb) Phospho-tau202 and 205 (p-tau202 and 205) hFc/mFc Details
    GMP-h-p-tau212/214-Ab Anti-human p-tau212 and 214 monoclonal antibody(mAb) Phospho-tau212 and 214 (p-tau212 and 214) hFc/mFc Details
    GMP-h-p-tau413-Ab Anti-human p-tau413 monoclonal antibody(mAb) Phospho-tau413 (p-tau413) hFc/mFc Details
    GMP-h-p-tau422-Ab Anti-human p-tau422 monoclonal antibody(mAb) Phospho-tau422 (p-tau422) hFc/mFc Details
    GMP-h-Tau-Ab Anti-human Tau monoclonal antibody(mAb) tau proteins (Tau) hFc/mFc Details

    Target/Biomarker information

    Alzheimer Disease (AD)  Recent publications and meeting presentations bolster the evidence that upticks in p-tau181, p-tau217, and p-tau231 occur soon after Aβ begins to accumulate in the brain. That’s years before neurofibrillary tangles can be detected by PET, and decades before symptoms. Researchers predict these markers, which also distinguish AD from other forms of dementia, will lead to more robust tests than those based on Aβ, because of their larger dynamic range—phosphorylated fragments of tau climb by as much as 10-fold in the blood as AD progresses, whereas the Aβ42/40 ratio falls by about 15 percent at most.



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    Comments


    James Hernandez 2023-02-08 07:16:48
    It provides reliable results and is suitable for use in the analysis of tauopathies and other neurodegenerative diseases.

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