Mycoplasma hyosynoviae antibody and antigen (recombinant protein)

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Diagnostic anti-Mycoplasma hyosynoviae antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease arthritis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P094-Ag01	Recombinant Mycoplasma hyosynoviae protein	$3090.00
GMP-VT-P094-Ab01	Anti-Mycoplasma hyosynoviae mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P094-Ab02	Anti-Mycoplasma hyosynoviae mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P094-Ag01
Product Name	Recombinant Mycoplasma hyosynoviae protein
Pathogen	Mycoplasma hyosynoviae
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Mycoplasma hyosynoviae antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Mycoplasma hyosynoviae level test of animal Swine/Porcine/Pig infectious disease with arthritis.
Tag	His
Product description	Recombinant Mycoplasma hyosynoviae proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P094-Ab01,GMP-VT-P094-Ab02
Pathogen	Mycoplasma hyosynoviae
Product Name	Anti-Mycoplasma hyosynoviae mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Mycoplasma hyosynoviae antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycoplasma hyosynoviae antibodies in Mycoplasma hyosynoviae level test of animal Swine/Porcine/Pig infectious disease with arthritis.
Product description	Anti-Mycoplasma hyosynoviae mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycoplasma hyosynoviae antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Mycoplasma hyosynoviae is a prokaryotic bacterium that primarily infects swine, causing joint inflammation and lameness. It is a member of the class Mollicutes and the family Mycoplasmataceae. Despite its small size, this bacterium can cause significant economic losses in the pork industry. As a result, there has been significant interest in understanding the biology of this pathogen and developing effective control measures.

The genome of Mycoplasma hyosynoviae has been sequenced, revealing a small genome size of approximately 860,000 base pairs. The genome encodes for approximately 667 protein-coding genes, including several that play a role in the pathogenicity of this bacterium. One such gene is the vsp gene, which encodes variable surface proteins. These proteins help the bacterium evade the host's immune system by undergoing rapid antigenic variation. This allows the bacterium to avoid recognition and neutralization by the immune system, making it challenging to develop vaccines against this pathogen.

Another critical gene associated with Mycoplasma hyosynoviae is the 16S rRNA gene, which codes for the ribosomal subunit. This gene is often used as a target for diagnostic PCR-based assays due to its high level of conservation across different strains of Mycoplasma hyosynoviae. Additionally, some methods target the p36 protein, a membrane-associated protein specific to Mycoplasma hyosynoviae. The ability to detect this pathogen at an early stage is critical for effective management and control of the disease in swine herds.

Swine are the primary hosts infected by Mycoplasma hyosynoviae, leading to joint inflammation and lameness. This pathology of swine is commonly referred to as swine arthritis. The disease primarily affects young pigs, with symptoms appearing between 3 and 12 weeks of age. The bacterium is transmitted through contact with infected animals or through contaminated equipment. Infected pigs may be asymptomatic carriers, making it challenging to control the spread of the disease within a herd.

In addition to swine arthritis, Mycoplasma hyosynoviae is also known to cause pneumonia, endocarditis, and reproductive disorders. In pigs, reproductive disorders can lead to lower litter sizes and decreased growth rates, further impacting the economic impact of this pathogen on the pork industry.

Various diagnostic methods have been developed for detecting Mycoplasma hyosynoviae infections. Serological tests such as enzyme-linked immunosorbent assay (ELISA) can detect antibodies against Mycoplasma hyosynoviae. PCR-based techniques such as real-time PCR and nested PCR can detect DNA from the pathogen. These methods target the vsp and 16S rRNA genes, as well as the p36 protein. Additionally, culture-based methods can be used to isolate the bacterium from infected tissues, although these methods can be time-consuming and challenging due to the fastidious nature of this bacterium.

In conclusion, Mycoplasma hyosynoviae is a pathogenic bacterium that primarily affects swine. The genome of this bacterium has been sequenced, revealing important genes involved in pathogenicity and virulence. Various diagnostic methods are available for detecting this pathogen, but early detection is critical for effective management and control of the disease. Further research on the biology of this pathogen is necessary to develop more effective control measures and vaccines.

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