Avian metapneumovirus antibody and antigen (recombinant protein)

Diagnostic anti-Avian metapneumovirus antibodies pairs and antigen for animal health (animal Avian/Bird/Poultry infectious disease Avian metapneumovirus infection) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P195-Ag01 Recombinant Avian metapneumovirus protein $3090.00
GMP-VT-P195-Ab01 Anti-Avian metapneumovirus mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P195-Ab02 Anti-Avian metapneumovirus mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P195-Ag01
Product Name Recombinant Avian metapneumovirus protein
Pathogen Avian metapneumovirus
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Avian metapneumovirus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Avian metapneumovirus level test of animal Avian/Bird/Poultry infectious disease with Avian metapneumovirus infection.
Tag His
Product description Recombinant Avian metapneumovirus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P195-Ab01,GMP-VT-P195-Ab02
Pathogen Avian metapneumovirus
Product Name Anti-Avian metapneumovirus mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Avian metapneumovirus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Avian metapneumovirus antibodies in Avian metapneumovirus level test of animal Avian/Bird/Poultry infectious disease with Avian metapneumovirus infection.
Product description Anti-Avian metapneumovirus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Avian metapneumovirus antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Avian Encephalomyelitis Virus: Unveiling the Microscopic Menace

    Names of the Pathogen:

    The Avian Encephalomyelitis virus, denoted by the acronym AEV, is a microscopic entity with a few distinct monikers within the scientific community. Among its aliases are the Epidemic Tremor virus and the Encephalomyelitis Virus of Turkeys.

    Pathogen Classification:

    Avian Encephalomyelitis virus belongs to the Picornaviridae family, a taxonomic group of small, non-enveloped, positive-sense single-stranded RNA viruses. As a viral entity, it is classified as an infectious microorganism, presenting unique characteristics that distinguish it from other pathogens.

    Pathogen Structure:

    The structural composition of AEV is both elegant and straightforward. At its core, it comprises a single-stranded positive-sense RNA genome ensconced within a protective protein coat called the capsid. The genomic content of AEV primarily resides within its RNA, which encodes a variety of viral proteins pivotal to its functioning.

    Hosts Infected and Diseases:

    Avian Encephalomyelitis virus exhibits a predilection for avian species, particularly chickens and turkeys. When AEV infiltrates its avian hosts, it sets in motion a cascade of biological events that culminate in a condition known as Avian Encephalomyelitis. This ailment is classified as a neurotropic disease, signifying its neurological impact on afflicted avian populations. The clinical manifestations of this disease encompass ataxia, tremors, and paralysis, which can severely compromise the affected birds' health and overall wellbeing.

    Diagnostic Methods:

    Detecting the presence of Avian Encephalomyelitis virus is a matter of utmost importance in veterinary and avian health management. Various diagnostic methods have been developed to achieve this crucial objective. These methods include:

    Serological Assays: Serological assays are instrumental in identifying the presence of AEV-specific antibodies generated by the avian host in response to infection. One of the most prominent serological tests employed is ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive technique that can detect the presence of AEV antibodies with precision.

    PCR (Polymerase Chain Reaction): PCR-based diagnostic techniques are powerful tools used to amplify specific genomic sequences of the Avian Encephalomyelitis virus. This amplification process enables the sensitive detection and quantification of AEV genetic material. The application of PCR has greatly enhanced the efficiency and accuracy of AEV detection, making it a preferred choice for many researchers and diagnosticians.

    RT-qPCR (Reverse Transcription quantitative PCR): This specialized technique is invaluable for the detection and quantification of RNA sequences, which is especially relevant for RNA viruses such as AEV. It involves reverse transcription to convert viral RNA into DNA, followed by quantitative PCR to precisely measure the quantity of the virus. This method offers unparalleled sensitivity in AEV detection.

    Nucleic Acid Hybridization Assays: Nucleic acid hybridization assays are diagnostic tests that employ complementary nucleic acid sequences to identify specific genes or RNA sequences of the Avian Encephalomyelitis virus. These assays are designed to target and bind to regions of the AEV genome, enabling the detection and identification of the virus.

    Targeted Genes or Proteins:

    In PCR-based methods and nucleic acid hybridization assays, specific genomic regions of the Avian Encephalomyelitis virus are targeted. These regions may encompass genes responsible for encoding structural proteins, viral enzymes, or other critical components of the virus. The choice of targeted genes or sequences may vary depending on the design of the diagnostic assay and the specific strain of AEV under investigation. This flexibility allows diagnosticians to tailor their approach to the unique genetic makeup of the virus in question.



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