Ecephalitis antibody and antigen (recombinant protein)

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Diagnostic anti-Ecephalitis antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease Ecephalitis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P103-Tg001-Ag01	Recombinant Ecephalitis protein	$3090.00
GMP-VT-P103-Tg001-Ab01	Anti-Ecephalitis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P103-Tg001-Ab02	Anti-Ecephalitis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P103-Tg001-Ab03	Anti-Ecephalitis human monoclonal antibody (mAb)	$3090.00
GMP-VT-P103-Tg001-Ab04	Anti-Ecephalitis human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P103-Tg001-Ag01
Product Name	Recombinant Ecephalitis protein
Pathogen	Ecephalitis
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Ecephalitis antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Ecephalitis level test of animal Swine/Porcine/Pig infectious disease with Ecephalitis.
Tag	His
Product description	Recombinant Ecephalitis proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P103-Tg001-Ab01,GMP-VT-P103-Tg001-Ab02
Pathogen	Ecephalitis
Product Name	Anti-Ecephalitis mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Ecephalitis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Ecephalitis antibodies in Ecephalitis level test of animal Swine/Porcine/Pig infectious disease with Ecephalitis.
Product description	Anti-Ecephalitis mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Ecephalitis antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P103-Tg001-Ab03,GMP-VT-P103-Tg001-Ab04
Pathogen	Ecephalitis
Product Name	Anti-Ecephalitis human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Ecephalitis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Ecephalitis antibodies in Ecephalitis level test of animal Swine/Porcine/Pig infectious disease with Ecephalitis.
Product description	Anti-Ecephalitis mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Actinobacillus pleuropneumoniae (APP) is a bacterium that significantly impacts the swine industry, causing a highly contagious and often fatal respiratory disease known as porcine pleuropneumonia. This pathogen's detailed exploration encompasses its taxonomy, structure, pathogenic mechanisms, the impact on hosts, associated diseases, and diagnostic strategies employed for its identification.

Taxonomy and Classification:

APP belongs to the phylum Proteobacteria, class Gammaproteobacteria, order Pasteurellales, family Pasteurellaceae. It is a Gram-negative, facultatively anaerobic coccobacillus. Eleven serotypes (1-11) have been identified, exhibiting variations in virulence and geographic distribution.

Pathogen Structure and Virulence Factors:

APP's pathogenicity is attributed to various virulence factors. Among the most notable are the Apx (Actinobacillus pleuropneumoniae cytotoxins) toxins. ApxI, ApxII, and ApxIII are key virulence factors responsible for causing lung lesions and cellular damage in the host. These toxins contribute to the characteristic pathology of porcine pleuropneumonia by targeting and damaging pulmonary epithelial cells.

Additionally, lipopolysaccharides (LPS) on the bacterial cell surface trigger inflammatory responses in the host, leading to a cascade of immune reactions, exacerbating the severity of the disease.

Impact on Hosts and Associated Diseases:

APP primarily infects domestic pigs (swine). The clinical manifestation of porcine pleuropneumonia varies in severity, ranging from acute to chronic. Infected animals often display symptoms such as coughing, labored breathing, fever, and pleurisy. The disease can lead to significant economic losses in affected swine populations due to reduced productivity and mortality rates.

Diagnostic Methods:

Accurate and timely diagnosis is crucial for disease management. Several diagnostic methods are employed:

Polymerase Chain Reaction (PCR): PCR-based tests amplify specific genes of APP, like the ApxIV gene, for rapid and precise detection in clinical samples.

Enzyme-Linked Immunosorbent Assay (ELISA): This serological test detects antibodies or antigens against APP in blood samples, aiding in diagnosis.

Nucleic Acid Hybridization: Utilizing nucleic acid probes, this method detects APP DNA in clinical samples.

Culturing and Isolation: Though less common due to its time-consuming nature, culturing samples on specific growth media can help isolate APP for further analysis.

Prevention and Control:

Preventing APP infection involves biosecurity measures, including quarantine, vaccination, and hygiene protocols to limit the pathogen's spread. Vaccines targeting prevalent serotypes have been developed to provide immunity in susceptible pig populations.

Research and Future Prospects:

Ongoing research focuses on understanding APP's genetic diversity, virulence factors, and vaccine development to enhance disease prevention and control strategies. This involves studying new serotypes, investigating pathogen-host interactions, and refining diagnostic tools for better accuracy and efficiency.

Understanding Actinobacillus pleuropneumoniae's intricate mechanisms, its impact on swine health, and the development of effective diagnostic and control measures is essential in maintaining swine health and safeguarding the global swine industry from the detrimental effects of porcine pleuropneumonia.

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