Transmissible gastroenteritis coronavirus Glycoprotein S antibody and antigen (recombinant protein)

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Diagnostic anti-Transmissible gastroenteritis coronavirus Glycoprotein S antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease viral enteritis and diarrhea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P109-Tg001-Ag01	Recombinant Transmissible gastroenteritis coronavirus Glycoprotein S protein	$3090.00
GMP-VT-P109-Tg001-Ab01	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P109-Tg001-Ab02	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P109-Tg001-Ab03	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S human monoclonal antibody (mAb)	$3090.00
GMP-VT-P109-Tg001-Ab04	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P109-Tg001-Ag01
Product Name	Recombinant Transmissible gastroenteritis coronavirus Glycoprotein S protein
Pathogen	Transmissible gastroenteritis coronavirus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Transmissible gastroenteritis coronavirus level test of animal Swine/Porcine/Pig infectious disease with viral enteritis and diarrhea.
Tag	His
Product description	Recombinant Transmissible gastroenteritis coronavirus Glycoprotein S proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P109-Tg001-Ab01,GMP-VT-P109-Tg001-Ab02
Pathogen	Transmissible gastroenteritis coronavirus
Product Name	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Transmissible gastroenteritis coronavirus Glycoprotein S antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Transmissible gastroenteritis coronavirus antibodies in Transmissible gastroenteritis coronavirus level test of animal Swine/Porcine/Pig infectious disease with viral enteritis and diarrhea.
Product description	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Transmissible gastroenteritis coronavirus antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P109-Tg001-Ab03,GMP-VT-P109-Tg001-Ab04
Pathogen	Transmissible gastroenteritis coronavirus
Product Name	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Transmissible gastroenteritis coronavirus Glycoprotein S antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Transmissible gastroenteritis coronavirus antibodies in Transmissible gastroenteritis coronavirus level test of animal Swine/Porcine/Pig infectious disease with viral enteritis and diarrhea.
Product description	Anti-Transmissible gastroenteritis coronavirus Glycoprotein S mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Taxonomy and Classification:

Actinobacillus pleuropneumoniae (APP) is a Gram-negative bacterium known for causing porcine pleuropneumonia, a highly contagious respiratory disease in swine. Understanding the taxonomy and classification of APP is fundamental to comprehending its characteristics and behavior.

APP belongs to the phylum Proteobacteria, placing it within the same bacterial group as other notable pathogens like Escherichia coli and Salmonella. More specifically, it falls under the class Gammaproteobacteria, order Pasteurellales, and family Pasteurellaceae. This classification places it in the same family as other significant veterinary pathogens, such as Mannheimia haemolytica and Pasteurella multocida.

Within the genus Actinobacillus, APP stands out as a prominent pathogen in swine. It's essential to recognize that there are multiple serotypes (1-11) of APP, and these serotypes exhibit variations in virulence and geographic distribution. Understanding the serotype of the infecting strain is crucial for disease management and vaccine development.

Pathogen Structure and Virulence Factors:

To comprehend how APP affects swine and causes porcine pleuropneumonia, it's vital to examine its structure and the virulence factors that enable it to infect and damage its host.

APP is a coccobacillus, a type of bacterium that is intermediate in shape between a coccus (spherical) and a bacillus (rod-shaped). This shape is common among many pathogens in the Pasteurellaceae family and plays a role in their ability to invade and persist in host tissues.

A key to APP's pathogenicity lies in its production of cytotoxins known as Apx toxins. There are three primary Apx toxins: ApxI, ApxII, and ApxIII. These toxins are crucial for causing lung lesions and cellular damage in the host. ApxI and ApxII toxins contribute to the characteristic pathology of porcine pleuropneumonia by targeting and damaging pulmonary epithelial cells. ApxIII toxin, while less studied, also plays a role in the disease's development.

In addition to Apx toxins, lipopolysaccharides (LPS) on the bacterial cell surface play a significant role in APP's pathogenicity. LPS is a component of the outer membrane of Gram-negative bacteria and acts as a potent trigger for inflammatory responses in the host. This leads to a cascade of immune reactions, exacerbating the severity of the disease.

Impact on Hosts and Associated Diseases:

Porcine pleuropneumonia, caused by APP, is a significant concern in the swine industry due to its impact on host animals. The clinical manifestations of the disease can vary in severity, ranging from acute to chronic. Infected pigs often exhibit symptoms such as coughing, labored breathing, fever, and pleurisy, which is inflammation of the membranes surrounding the lungs.

In severe cases, porcine pleuropneumonia can lead to high mortality rates and substantial economic losses. Diseased animals may experience reduced weight gain and impaired feed conversion, leading to decreased productivity in swine farms. In addition to the acute form, there is also a chronic form of the disease, which can result in long-term health issues and reduced performance in affected pigs.

Diagnostic Methods:

Accurate and timely diagnosis of porcine pleuropneumonia is essential for disease management. Various diagnostic methods are employed by veterinarians and researchers to identify and confirm the presence of APP:

Polymerase Chain Reaction (PCR): PCR-based tests are used to amplify specific genes of APP, such as the ApxIV gene, allowing for rapid and precise detection in clinical samples.

Enzyme-Linked Immunosorbent Assay (ELISA): This serological test is designed to detect antibodies or antigens against APP in blood samples, aiding in diagnosis.

Nucleic Acid Hybridization: Utilizing nucleic acid probes, this method detects APP DNA in clinical samples, providing another approach to confirm the infection.

Culturing and Isolation: Although less common due to its time-consuming nature, culturing samples on specific growth media can help isolate APP for further analysis and strain characterization.

Prevention and Control:

Preventing APP infection is a multifaceted challenge but is crucial for maintaining swine health and productivity. Key strategies for prevention and control include:

Biosecurity Measures: Implementing strict biosecurity measures to prevent the introduction and spread of the bacterium within swine herds.

Vaccination: Several vaccines targeting prevalent serotypes of APP have been developed to provide immunity in susceptible pig populations. These vaccines aim to reduce the severity and impact of porcine pleuropneumonia.

Hygiene Protocols: Maintaining good hygiene practices in swine farms and controlling factors that can stress the animals, such as overcrowding, can help reduce the likelihood of APP infections.

Quarantine: Isolating and monitoring newly introduced animals can prevent the introduction of the pathogen to existing swine populations.

Research and Future Prospects:

Ongoing research in the field of porcine pleuropneumonia and APP continues to advance our understanding of this pathogen and the disease it causes. Areas of active research include:

Genetic Diversity: Scientists are studying the genetic diversity of APP strains to better understand how different serotypes vary in terms of virulence and geographical distribution.

Virulence Factors: Investigating the mechanisms by which APP's virulence factors, such as Apx toxins, interact with the host and cause disease.

Vaccine Development: Developing new and improved vaccines that target multiple serotypes and provide broader protection against porcine pleuropneumonia.

Diagnostic Tools: Refining diagnostic tools to improve their accuracy and efficiency, which is critical for early detection and management of the disease.

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