Infectious Haematopoietic Necrosis Virus Nucleoprotein antibody and antigen (recombinant protein)

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Diagnostic anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein antibodies pairs and antigen for animal health (animal Salmonid fish infectious disease infectious hematopoietic necrosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P232-Tg001-Ag01	Recombinant Infectious Haematopoietic Necrosis Virus Nucleoprotein protein	$3090.00
GMP-VT-P232-Tg001-Ab01	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P232-Tg001-Ab02	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P232-Tg001-Ab03	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein human monoclonal antibody (mAb)	$3090.00
GMP-VT-P232-Tg001-Ab04	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P232-Tg001-Ag01
Product Name	Recombinant Infectious Haematopoietic Necrosis Virus Nucleoprotein protein
Pathogen	Infectious Haematopoietic Necrosis Virus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Infectious Haematopoietic Necrosis Virus level test of animal Salmonid fish infectious disease with infectious hematopoietic necrosis.
Tag	His
Product description	Recombinant Infectious Haematopoietic Necrosis Virus Nucleoprotein proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P232-Tg001-Ab01,GMP-VT-P232-Tg001-Ab02
Pathogen	Infectious Haematopoietic Necrosis Virus
Product Name	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Infectious Haematopoietic Necrosis Virus Nucleoprotein antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Haematopoietic Necrosis Virus antibodies in Infectious Haematopoietic Necrosis Virus level test of animal Salmonid fish infectious disease with infectious hematopoietic necrosis.
Product description	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Haematopoietic Necrosis Virus antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P232-Tg001-Ab03,GMP-VT-P232-Tg001-Ab04
Pathogen	Infectious Haematopoietic Necrosis Virus
Product Name	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Infectious Haematopoietic Necrosis Virus Nucleoprotein antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Haematopoietic Necrosis Virus antibodies in Infectious Haematopoietic Necrosis Virus level test of animal Salmonid fish infectious disease with infectious hematopoietic necrosis.
Product description	Anti-Infectious Haematopoietic Necrosis Virus Nucleoprotein mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

IHNV is a viral pathogen that primarily infects salmonid fish, including rainbow trout, Atlantic salmon, and Pacific salmon. The virus was first identified in the United States in the 1950s and has since been found in various regions worldwide. IHNV belongs to the family Rhabdoviridae, which includes several other fish viruses and the rabies virus.

The genome of IHNV is composed of negative-sense single-stranded RNA and contains five genes: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). N protein encapsulates viral RNA, P protein interacts with N protein to form ribonucleoprotein complexes, M protein plays an important role in virion assembly, and G protein facilitates viral entry by binding to host cells. The L protein serves as an RNA-dependent RNA polymerase that allows the virus to replicate its RNA genome.

IHNV causes a severe disease known as infectious hematopoietic necrosis (IHN), which affects the internal organs and tissues of infected fish. The disease is characterized by anemia, petechial hemorrhages, and necrosis. Infected fish may exhibit external clinical signs such as darkened skin, distended abdomen, and exophthalmia. These symptoms are a result of the damage caused to the hematopoietic system and other organs.

The pathogenesis of IHN involves viral replication in susceptible fish tissues, leading to the induction of the host immune response. The immune response can cause the destruction of infected cells through apoptosis, leading to tissue damage and the release of inflammatory cytokines. In addition, the virus can infect hematopoietic stem cells, leading to a decline in the number of circulating blood cells and anemia.

Several diagnostic methods have been developed to identify IHNV infections in salmonid fish. These methods include PCR (polymerase chain reaction) and ELISA (enzyme-linked immunosorbent assay), which detect viral RNA or antibodies. These diagnostic methods target the N and G genes of IHNV. In addition, virus isolation and histopathological examination can also be utilized to diagnose IHN infections. Virus isolation involves the cultivation of viral particles on cell culture, while histopathological examination involves the microscopic analysis of tissue sections to identify characteristic lesions.

The control of IHN relies on the implementation of various measures, such as fish vaccination, biosecurity, and quarantine protocols. Vaccination is the most effective control measure, as it can stimulate the production of specific antibodies that can neutralize the virus. Biosecurity measures, such as preventing the introduction of infected fish or equipment, can also prevent the spread of the virus between farms. Quarantine protocols involve isolating new fish arrivals to monitor them for signs of infection before introducing them into the main population.

In conclusion, IHNV is a highly pathogenic virus that infects salmonid fish, causing severe disease known as infectious hematopoietic necrosis (IHN). The virus primarily targets the hematopoietic system and other organs, leading to anemia, petechial hemorrhages, and necrosis. Several diagnostic methods have been developed to identify IHNV infections, including PCR, ELISA, virus isolation, and histopathological examination. The control of IHN relies on the implementation of various measures, such as fish vaccination, biosecurity, and quarantine protocols.

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