SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) (Cat No.:GM-CD14-Cell-iso-kit)

Isolate pristine Human Classical Monocytes (CD14++) with the SOLIDEX™-ISOEx Kit. Utilizing a superior Column-based Negative (Untouched) selection strategy, this kit depletes non-target cells from PBMC using optimized Magnetic Beads. The eluted cells are free of antibodies and beads, ensuring Zero Activation and preserving their native phenotype. Achieve High Purity and high viability for sensitive downstream applications like macrophage differentiation.

SOLIDEX™-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
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Order information


Catalog No. Product Name Size Price(In USD)
GM-CD14-Cell-iso-kit-10T SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 10 Tests 190
GM-CD14-Cell-iso-kit-50T SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 50 Tests 570
GM-CD14-Cell-iso-kit-100T SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 100 Tests 1004
GM-CD14-Cell-iso-kit-750T SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 750 Tests

Description


Cat No. GM-CD14-Cell-iso-kit
Product Name SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Description SOLIDEX™-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
Application SOLIDEX™-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-Tg-hg-T23212-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: -50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Cell population CD14+CD16- Cell
Storage Store at 2℃-8℃

Application

  • Differentiation Studies: Generation of monocyte-derived macrophages (M1/M2) or dendritic cells (Mo-DCs) in vitro.
  • Innate Immunity Research: Analysis of TLR signaling pathways and cytokine secretion profiling.
  • Single-Cell Sequencing (scRNA-seq): High-quality, stress-free cells minimize transcriptomic artifacts caused by activation.
  • Migration and Chemotaxis Assays: utilizing native, unblocked receptors.

Protocol

  1. Preparation: Prepare a single-cell suspension from cryopreserved or fresh PBMCs.
  2. Labeling: Add the Biotin-Antibody Cocktail (targets non-CD14 cells: T cells, B cells, NK cells, etc.). Incubate.
  3. Bead Binding: Add Anti-Biotin Microbeads to bind the labeled non-target cells. Incubate.
  4. Separation: Apply the suspension to the separation column placed in a magnetic field.
  5. Elution: Unlabeled CD14+ Classical Monocytes pass through the column freely and are collected (Untouched).

Validation Data

High-purity isolation of classical monocytes from human peripheral blood mononuclear cells (PBMC) using the SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)

Catalog No. GM-CD14-Cell-iso-kit
In this experiment, the SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) was used to isolate and purify classical monocytes from cryopreserved PBMCs. First, non-monocytic cells were specifically labeled with a biotin-conjugated antibody cocktail (Antibody Cocktail), followed by incubation with anti-biotin nano-magnetic beads. The purity of the cell populations before and after isolation was analyzed by flow cytometry. The purity of CD14+ classical monocytes in the flow-through fraction increased from 21.7% to 92.1%. The experimental results demonstrate that this kit effectively removes contaminating cells, yielding high-purity, untouched (antibody-unbound) classical monocytes.

Figure 14. High-purity isolation of classical monocytes from human peripheral blood mononuclear cells (PBMC) using the SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based). Flow cytometry results show that the purity of CD14+ classical monocytes increased from 21.7% to 92.1%.

ISOEx Cell Isolation Kit & Nanobeads validation
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Background

Why Choose SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit?

  • True "Untouched" Isolation: Target monocytes are never bound by antibodies or beads, preventing Fc-receptor activation and preserving differentiation potential.
  • Exceptional Purity: The Column-based matrix acts as a physical filter, allowing rigorous washing of contaminants to achieve >90% purity compared to column-free methods.
  • Preserved Physiology: Gentle isolation ensures cells remain in a resting state, critical for accurate immunological studies.
  • Rapid Workflow: Isolate cells in under 45 minutes with a simple, standardized protocol.

FAQ

What are the sample requirements?
We recommend using fresh or cryopreserved Human PBMCs. For cryopreserved samples, ensure high viability (>90%) and proper DNase treatment during thawing to prevent clumping. Leukapheresis products are also compatible.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (Negative Selection) kit, the CD14 receptors and other surface markers are free and not blocked by magnetic beads or antibodies. You can proceed directly to staining.
How does the Column-based separation work?
The column contains a ferromagnetic matrix (spheres). When placed in a magnetic field, the matrix amplifies the magnetic force by 10,000-fold, effectively retaining the magnetically labeled non-target cells while unlabeled monocytes pass through.
Are the separation columns reusable?
No. The columns are designed for single-use to ensure sterility and prevent cross-contamination between patient samples.
What is the advantage of Column-based over Column-free methods?
Column-based methods typically offer higher purity. The column allows for washing of the retained cell mass, ensuring that only the specific "Untouched" cells elute. Column-free methods often struggle with non-specific entrapment of target cells in the magnetic clump, leading to lower purity or recovery.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX™-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.