Biotin (BT) antibody and antigen (hapten, BSA/OVA conjugated)

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-SMT-219-Ag01	BSA-Biotin (BT)	756
GMP-SMT-219-Ag02	OVA-Biotin (BT)	756
GMP-SMT-219-Ab01	Anti-human Biotin (BT) mouse monoclonal antibody (mAb)	1953
GMP-SMT-219-Ab02	Anti-human Biotin (BT) human monoclonal antibody (mAb)	1953

Size： 1mg | 10mg | 100mg

Product Description

Cat No. of Products	GMP-SMT-219-Ag01
Product Name	BSA-Biotin (BT)
Target/Biomarker	Biotin(BT)
Expression platform	Synthetic
Bioactivity validation	Biotin (BT) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in BT level test of Metabolic diseases (Biotin deficiency) and related syndrome evaluation.
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-Biotin (BT) / OVA-Biotin (BT) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-219-Ag02
Product Name	OVA-Biotin (BT)
Target/Biomarker	Biotin(BT)
Expression platform	Synthetic
Bioactivity validation	Biotin (BT) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in BT level test of Metabolic diseases (Biotin deficiency) and related syndrome evaluation.
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-Biotin (BT) / OVA-Biotin (BT) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-219-Ab01
Product Name	Anti-human Biotin (BT) mouse monoclonal antibody (mAb)
Target/Biomarker	Biotin(BT)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Human Biotin (BT) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other Biotin (BT) antibodies in BT level test of Metabolic diseases (Biotin deficiency) and related syndrome evaluation.
Tag	mFc
Products description	Anti-human Biotin (BT) monoclonal antibody (mAb) is a monoclonal antibody produced by hybridoma technology.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-219-Ab02
Product Name	Anti-human Biotin (BT) human monoclonal antibody (mAb)
Target/Biomarker	Biotin(BT)
Expression platform	CHO
Isotypes	Human IgG1
Bioactivity validation	Human Biotin (BT) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other Biotin (BT) antibodies in BT level test of Metabolic diseases (Biotin deficiency) and related syndrome evaluation.
Tag	mFc
Products description	Anti-human Biotin (BT) monoclonal antibody (mAb) is a monoclonal antibody produced by hybridoma technology.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Target/Biomarker information

Biotin in In Vitro Diagnosis (IVD): Biotin, also scientifically referred to as Vitamin B7 or Vitamin H, emerges as a paramount constituent within the intricate framework of in vitro diagnosis (IVD). Its role within this domain is multifaceted, manifesting as a cornerstone in numerous assay systems and diagnostic methodologies. Biotin's intrinsic significance primarily emanates from its capacity to serve as a conjugate or label in immunoassays and other sophisticated detection mechanisms harnessed extensively in IVD. In the specific context of IVD, Biotin finds profound utility as a conjugate. It becomes covalently linked to carrier proteins, such as Bovine Serum Albumin (BSA) or Ovalbumin (OVA), resulting in the formation of Biotinylated antigens. These Biotinylated antigens then embark on a pivotal journey as they are deployed to ascertain the presence or quantify the concentration of particular antibodies or analytes within patient specimens. A remarkable facet of Biotin lies in its exceptional binding affinity, particularly with Avidin or Streptavidin proteins, which renders it indispensable in assays of paramount importance. Assay methodologies such as enzyme-linked immunosorbent assays (ELISAs) and chemiluminescent immunoassays (CLIAs) stand as exemplars, where Biotinylated reagents underpin precision, sensitivity, and ultimately, the accuracy of diagnostic detection. Significance of Biotin Measurement in IVD: a. Quality Assurance (QA) and Control (QC): The meticulous measurement of Biotin holds a pivotal role in ensuring the veracity of in vitro diagnostic assays. This veracity hinges upon the quality and consistency of Biotinylated reagents, encompassing Biotin-conjugated antigens and antibodies. Rigorous measurement, therefore, assumes an indispensable role in quality assurance (QA) and control (QC) protocols, bolstering the generation of dependable assay outcomes. As Biotin is an elemental component of these reagents, precise measurement becomes pivotal to ascertain the optimal concentration and functionality of these diagnostic entities. b. Augmented Assay Sensitivity and Specificity: Within the intricate tapestry of in vitro diagnostic assays, the precise measurement of Biotin levels stands as a linchpin in optimizing assay parameters. Fine-tuning the concentration of Biotinylated reagents within assays is an imperative step in achieving heightened sensitivity and specificity. By deciphering the precise Biotin levels required, diagnostic tests become adept at discriminating minute variations in analyte concentrations, thereby augmenting their clinical utility and diagnostic accuracy. c. Mitigating Biotin Interference: Biotin interference represents a substantial concern in specific clinical assays. Elevated Biotin levels within patient specimens can potentially induce spurious results, leading to erroneous clinical interpretations. Precise measurement of Biotin levels within patient samples facilitates the identification of potential interference instances, empowering laboratory professionals to implement necessary protocol adjustments and ensure the reliability of assay results. d. Patient Biotin Level Monitoring: In selected clinical scenarios, the monitoring of Biotin levels within patients' biological matrices assumes clinical relevance. Excessive Biotin supplementation, either through dietary sources or supplements, can exert significant interference with laboratory test results. This interference is particularly pertinent in hormone assays. Comprehensive knowledge of a patient's Biotin levels enables healthcare practitioners to judiciously interpret test outcomes, ensuring that diagnostic conclusions are founded on accurate and unbiased data. In summation, the meticulous measurement of Biotin is an elemental and non-negotiable facet of in vitro diagnosis (IVD), pivotal for safeguarding the precision, sensitivity, and reliability of diagnostic assays reliant on Biotinylated reagents. It constitutes an indispensable element within the ambit of IVD, underpinning the scientific and clinical rigor of modern diagnostic practices. The careful consideration and precise measurement of Biotin levels serve as the bedrock upon which accurate clinical diagnoses are made, thereby shaping the landscape of healthcare and patient management.

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