Border disease Virus E2 antibody and antigen (recombinant protein)

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Diagnostic anti-Border disease Virus E2 antibodies pairs and antigen for animal health (animal Ovines/Sheep, Caprine/Goat infectious disease congenital diseases) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P085-Tg001-Ag01	Recombinant Border disease Virus E2 protein	$3090.00
GMP-VT-P085-Tg001-Ab01	Anti-Border disease Virus E2 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P085-Tg001-Ab02	Anti-Border disease Virus E2 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P085-Tg001-Ab03	Anti-Border disease Virus E2 human monoclonal antibody (mAb)	$3090.00
GMP-VT-P085-Tg001-Ab04	Anti-Border disease Virus E2 human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P085-Tg001-Ag01
Product Name	Recombinant Border disease Virus E2 protein
Pathogen	Border disease Virus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Border disease Virus E2 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Border disease Virus level test of animal Ovines/Sheep, Caprine/Goat infectious disease with congenital diseases.
Tag	His
Product description	Recombinant Border disease Virus E2 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P085-Tg001-Ab01,GMP-VT-P085-Tg001-Ab02
Pathogen	Border disease Virus
Product Name	Anti-Border disease Virus E2 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Border disease Virus E2 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Border disease Virus antibodies in Border disease Virus level test of animal Ovines/Sheep, Caprine/Goat infectious disease with congenital diseases.
Product description	Anti-Border disease Virus E2 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Border disease Virus antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P085-Tg001-Ab03,GMP-VT-P085-Tg001-Ab04
Pathogen	Border disease Virus
Product Name	Anti-Border disease Virus E2 human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Border disease Virus E2 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Border disease Virus antibodies in Border disease Virus level test of animal Ovines/Sheep, Caprine/Goat infectious disease with congenital diseases.
Product description	Anti-Border disease Virus E2 mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Border disease virus, commonly known as BDV, is a viral pathogen that affects domesticated ruminants such as cattle, sheep, and goats. BDV belongs to the family Flaviviridae and the Pestivirus genus. It is a single-stranded RNA virus with an enveloped structure.

BDV can cause severe clinical outcomes, depending on the virus strain and animal age. In adult animals, BDV infection may be asymptomatic or can result in immune suppression, reduced milk yield, and reproductive failure. In contrast, infection in young animals, especially those infected in the uterus, can lead to severe congenital abnormalities like cerebellar hypoplasia, which causes shaking and poor coordination.

The BDV genome consists of one open reading frame (ORF) encoding a single polyprotein precursor of approximately 3,900 amino acids. This polyprotein precursor is then cleaved by the viral proteases to produce ten individual viral proteins: three structural proteins - capsid (C), envelope glycoproteins E1 and E2, and the p7 protein; and seven non-structural proteins (NS2-3, NS4A, NS4B, NS5A, and NS5B). The C protein forms the viral core, where the RNA genome is contained, while E1 and E2 glycoproteins are responsible for viral attachment and entry into host cells.

BDV infection is widespread globally, with seroprevalence ranging from 0.3% to 99.9%. Transmission of BDV occurs through direct contact with infected animals, environmental contamination with urine, feces, and other bodily fluids of infected animals, or via vertical transmission from infected mothers to their offspring.

Diagnosis of BDV infection involves various methods, including serological tests, virus isolation, and nucleic acid detection. Serological tests detect antibodies against the virus and are appropriate for surveillance and control programs. ELISA is the most commonly used serological test for BDV, but it does not differentiate between BDV and other related Pestiviruses like bovine viral diarrhea virus (BVDV). On the other hand, virus isolation is a culture-based method for detecting the virus that can take several weeks to get a result. The nucleic acid-based methods, such as reverse-transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR, are increasingly popular due to their speed and specificity. These methods target specific regions of the viral genome, such as the 5' untranslated region (UTR), NS5B gene, or E2 gene.

Prevention and control of BDV infection require a combination of measures like vaccination, biosecurity, and monitoring. Vaccination is an effective strategy to reduce virus spread, protect animals from severe clinical outcomes, and prevent vertical transmission. Biosecurity measures include quarantining new animals entering the farm, cleaning and disinfection of shared equipment, and prevention of mixing of animals from different age groups. Monitoring for BDV infection should be done regularly in farms at high risk of BDV infections.

In conclusion, BDV is a viral pathogen that affects domesticated ruminants and causes both subclinical and severe clinical outcomes. Different diagnostic methods like serological tests, virus isolation, and nucleic acid-based methods are available for accurate diagnosis. Precautionary measures like biosecurity, vaccination, and monitoring are essential to prevent and control BDV infection.

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