Brachyspira hyodysenteriae Bhlp29.7 antibody and antigen (recombinant protein)

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Product information

Size： 1mg | 10mg | 100mg

Catalog No.	Description	US $ Price (per mg)	First Order Discount	First Order Discount Price
GMP-VT-P102-Tg001-Ag01	Recombinant Brachyspira hyodysenteriae Bhlp29.7 protein	3090
GMP-VT-P102-Tg001-Ab01	Anti-Brachyspira hyodysenteriae Bhlp29.7 mouse monoclonal antibody (mAb)	1953	20%	1562.4
GMP-VT-P102-Tg001-Ab02	Anti-Brachyspira hyodysenteriae Bhlp29.7 mouse monoclonal antibody (mAb)	1953	20%	1562.4
GMP-VT-P102-Tg001-Ab03	Anti-Brachyspira hyodysenteriae Bhlp29.7 mouse monoclonal antibody (mAb)	1953	20%	1562.4
GMP-VT-P102-Tg001-Ab04	Anti-Brachyspira hyodysenteriae Bhlp29.7 mouse monoclonal antibody (mAb)	1953	20%	1562.4

Shipping Costs: $360–$760
Antibodies: $360 Antigens: $760 (Elevated cost due to antigen heterogeneity, post-translational modifications, structural complexity, and specialized handling.)

Product Description

Cat No. of Products	GMP-VT-P102-Tg001-Ag01
Product Name	Recombinant Brachyspira hyodysenteriae Bhlp29.7 protein
Pathogen	Brachyspira Hyodysenteriae
Target/Biomarker	Bhlp29.7
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Brachyspira Hyodysenteriae Bhlp29.7 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Brachyspira Hyodysenteriae level test of animal Swine/Porcine/Pig with Brachyspira hyodysenteriae infection.
Tag	His
Products description	Recombinant Brachyspira hyodysenteriae Bhlp29.7 protein was expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-VT-P102-Tg001-Ab01, GMP-VT-P102-Tg001-Ab02, GMP-VT-P102-Tg001-Ab03, GMP-VT-P102-Tg001-Ab04
Product Name	Anti-Brachyspira hyodysenteriae Bhlp29.7 mouse monoclonal antibody (mAb)
Pathogen	Brachyspira Hyodysenteriae
Target/Biomarker	Bhlp29.7
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Brachyspira Hyodysenteriae Bhlp29.7 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Brachyspira Hyodysenteriae antibodies in Brachyspira Hyodysenteriae level test of animal Swine/Porcine/Pig with Brachyspira hyodysenteriae infection.
Tag	mFc
Products description	Anti-Brachyspira hyodysenteriae Bhlp29.7 mouse monoclonal antibody (mAb) is a monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Target/Biomarker information

Swine Dysentery (SD) is a severe mucohaemorhagic enteric disease of pigs caused by Brachyspira hyodysenteriae, which has a large impact on pig production and causes important losses due to mortality and sub-optimal performance. Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry. B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus. We previously determined that a B. hyodysenteriae strain binds to colon mucins in a manner that differs between pigs and mucin types. Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains). Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins. Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures. Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs. The results emphasize the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies.

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