Duck hepatitis virus VP1 protein antibody and antigen (recombinant protein)

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Diagnostic anti-Duck hepatitis virus VP1 protein antibodies pairs and antigen for animal health (animal Duck infectious disease Duck hepatitis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P176-Tg002-Ag01	Recombinant Duck hepatitis virus VP1 protein protein	$3090.00
GMP-VT-P176-Tg002-Ab01	Anti-Duck hepatitis virus VP1 protein mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P176-Tg002-Ab02	Anti-Duck hepatitis virus VP1 protein mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P176-Tg002-Ab03	Anti-Duck hepatitis virus VP1 protein human monoclonal antibody (mAb)	$3090.00
GMP-VT-P176-Tg002-Ab04	Anti-Duck hepatitis virus VP1 protein human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P176-Tg002-Ag01
Product Name	Recombinant Duck hepatitis virus VP1 protein protein
Pathogen	Duck hepatitis virus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Duck hepatitis virus VP1 protein antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Duck hepatitis virus level test of animal Duck infectious disease with Duck hepatitis.
Tag	His
Product description	Recombinant Duck hepatitis virus VP1 protein proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P176-Tg002-Ab01,GMP-VT-P176-Tg002-Ab02
Pathogen	Duck hepatitis virus
Product Name	Anti-Duck hepatitis virus VP1 protein mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Duck hepatitis virus VP1 protein antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Duck hepatitis virus antibodies in Duck hepatitis virus level test of animal Duck infectious disease with Duck hepatitis.
Product description	Anti-Duck hepatitis virus VP1 protein mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Duck hepatitis virus antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P176-Tg002-Ab03,GMP-VT-P176-Tg002-Ab04
Pathogen	Duck hepatitis virus
Product Name	Anti-Duck hepatitis virus VP1 protein human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Duck hepatitis virus VP1 protein antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Duck hepatitis virus antibodies in Duck hepatitis virus level test of animal Duck infectious disease with Duck hepatitis.
Product description	Anti-Duck hepatitis virus VP1 protein mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Infectious Bursal Disease Virus (IBDV): A Comprehensive Overview

Pathogen Name and Classification:

Infectious Bursal Disease Virus (IBDV) is a pathogenic virus that belongs to the Avibirnavirus genus within the Birnaviridae family. This viral family is characterized by non-enveloped, bisegmented double-stranded RNA genomes, making IBDV structurally unique among RNA viruses. IBDV primarily affects avian species, with chickens as the principal host. It is the etiological agent responsible for Infectious Bursal Disease (IBD), a highly contagious and economically significant disease affecting the poultry industry worldwide.

Viral Structure and Proteins:

The IBDV virion exhibits a relatively simple structure, comprising two segments: segment A and segment B. These segments encode essential proteins vital to the virus's life cycle.

Segment A: This segment encodes for the structural proteins of IBDV. The most notable among these is VP2 (Viral Protein 2), a major structural protein that plays a pivotal role in host cell attachment. The distinctive VP2 protein is responsible for eliciting a strong immune response in the host. Other structural proteins derived from segment A include VP3 and VP4, which contribute to the architecture of the viral capsid.

Segment B: This segment encodes non-structural proteins essential for the virus's replication and pathogenicity. VP1, an RNA-dependent RNA polymerase, is of particular significance. VP1 is pivotal for viral replication and transcription and is a primary target for antiviral research. Additionally, VP5 and VPX are non-structural proteins that have distinct roles in the viral life cycle. VP5 is involved in RNA encapsidation, while VPX influences the host's cellular environment and immune response.

Host Range and Associated Disease:

IBDV primarily infects avian species, both domestic and wild, with chickens being the primary host. The virus primarily targets the bursa of Fabricius, a crucial organ in the avian immune system. The bursa is responsible for the maturation of B lymphocytes, which play a central role in humoral immune responses. Infection with IBDV leads to immunosuppression, as the virus damages the bursal tissues, resulting in a decrease in antibody production.

The consequences of IBDV infection are severe, as the virus renders infected birds more susceptible to secondary infections. This not only increases the risk of opportunistic pathogens but also affects vaccine efficacy, as the bursa is the primary site of immune response development to vaccines in young chicks. Consequently, IBDV outbreaks can have a profound economic impact on the poultry industry due to increased mortality rates and decreased production efficiency.

Diagnostic Methods:

Several diagnostic techniques have been developed for the detection of IBDV, allowing for early identification and control of outbreaks. These methods include:

Polymerase Chain Reaction (PCR): PCR is a highly sensitive and specific method for detecting IBDV. Primers targeting specific genes within the viral genome, such as VP2 or VP1, are employed to amplify viral nucleic acids, allowing for the virus's genetic material to be detected and quantified.

Reverse Transcription PCR (RT-PCR): RT-PCR is used for the detection of viral RNA. By converting the viral RNA into complementary DNA (cDNA) using reverse transcriptase, followed by PCR amplification, this method offers a powerful tool for diagnosing IBDV.

Enzyme-Linked Immunosorbent Assay (ELISA): ELISA is a serological method that detects the presence of antibodies against IBDV in blood samples. This assay is especially valuable for monitoring the immune response of a flock to infection and vaccination.

Virus Isolation: Isolating the virus from infected tissue or bursal samples through cell culture is a traditional method used to diagnose IBDV. However, it is time-consuming and may be less sensitive than molecular techniques.

Immunohistochemistry: This method involves the use of specific antibodies to target viral antigens within bursal tissues. It can provide detailed information about the distribution of the virus in affected organs and is valuable for histological diagnosis.

Control and Prevention:

Preventing IBDV is critical to maintaining the health and productivity of poultry flocks. Control measures include:

Vaccination: Vaccination with live attenuated or inactivated vaccines is widely used to protect against IBDV. The timing of vaccination is crucial, as it must occur after maternal antibody protection wanes but before the bird becomes susceptible to clinical disease. Several commercial vaccines are available for IBD prevention.

Biosecurity: Strict biosecurity measures, such as controlling access to farms, disinfection, and the use of quarantine facilities, are essential to prevent the introduction of IBDV to poultry operations.

Genetic Resistance: Some chicken breeds exhibit genetic resistance to IBDV, which can be selectively bred for to enhance the overall flock's resistance to the disease.

Surveillance and Monitoring: Regular testing and monitoring of flocks for IBDV are essential to detect the virus early and implement control measures promptly.

Conclusion:

Infectious Bursal Disease Virus (IBDV) is a significant pathogen in the poultry industry, causing immunosuppression and economic losses. Understanding its structure, host range, and diagnostic methods is crucial for effective management and control. Through vaccination, biosecurity, genetic selection, and vigilant surveillance, the impact of IBDV can be minimized, safeguarding the health and productivity of poultry populations. Researchers and veterinarians continue to explore new strategies and treatments to combat this virus, as the poultry industry remains an essential component of global food security.

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