Borrelia burgdorferi sensu lato P41 (flagellin) antibody and antigen (recombinant protein)

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Diagnostic anti-Borrelia burgdorferi sensu lato P41 (flagellin) antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig infectious disease Borreliosis / Lyme disease) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P267-Tg001-Ag01	Recombinant Borrelia burgdorferi sensu lato P41 (flagellin) protein	$3090.00
GMP-VT-P267-Tg001-Ab01	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P267-Tg001-Ab02	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P267-Tg001-Ab03	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) human monoclonal antibody (mAb)	$3090.00
GMP-VT-P267-Tg001-Ab04	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P267-Tg001-Ag01
Product Name	Recombinant Borrelia burgdorferi sensu lato P41 (flagellin) protein
Pathogen	Borrelia burgdorferi sensu lato
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Borrelia burgdorferi sensu lato level test of animal Cat/Feline, Dog/Canine, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig infectious disease with Borreliosis / Lyme disease.
Tag	His
Product description	Recombinant Borrelia burgdorferi sensu lato P41 (flagellin) proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P267-Tg001-Ab01,GMP-VT-P267-Tg001-Ab02
Pathogen	Borrelia burgdorferi sensu lato
Product Name	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Borrelia burgdorferi sensu lato P41 (flagellin) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Borrelia burgdorferi sensu lato antibodies in Borrelia burgdorferi sensu lato level test of animal Cat/Feline, Dog/Canine, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig infectious disease with Borreliosis / Lyme disease.
Product description	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Borrelia burgdorferi sensu lato antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P267-Tg001-Ab03,GMP-VT-P267-Tg001-Ab04
Pathogen	Borrelia burgdorferi sensu lato
Product Name	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Borrelia burgdorferi sensu lato P41 (flagellin) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Borrelia burgdorferi sensu lato antibodies in Borrelia burgdorferi sensu lato level test of animal Cat/Feline, Dog/Canine, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig infectious disease with Borreliosis / Lyme disease.
Product description	Anti-Borrelia burgdorferi sensu lato P41 (flagellin) mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Borrelia burgdorferi sensu lato: A Comprehensive Examination

Introduction

Borrelia burgdorferi sensu lato is a complex of spirochetal bacteria, comprising various genospecies within the Borrelia genus. These microorganisms are of significant scientific and medical interest due to their causative role in Lyme disease, a vector-borne zoonosis that affects humans and other mammals. In this comprehensive examination, we will delve into the structure, classification, hosts, associated diseases, and diagnostic methods related to Borrelia burgdorferi sensu lato.

Classification and Structure

Borrelia burgdorferi sensu lato belongs to the phylum Spirochaetes, a group of unique bacteria characterized by their long, helically coiled cells with flexible cell walls, which enable them to move effectively through viscous environments. This distinct morphology is responsible for the bacteria's name 'spirochete,' derived from the Greek word 'spiros' (spiral) and 'chaitē' (hair).

The complex encompasses a range of genospecies, including Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia spielmanii, Borrelia bavariensis, and others. Each of these genospecies exhibits genetic variations that contribute to their unique biological properties and geographical distribution. For instance, Borrelia afzelii is often associated with rodents and birds, while Borrelia garinii is prevalent in avian reservoirs.

Pathogen Transmission

Borrelia burgdorferi sensu lato is primarily transmitted to mammals, including humans, through the bites of infected Ixodes ticks. These ticks serve as vectors, acquiring the bacteria from reservoir hosts, which can be small mammals or birds. When an infected tick attaches itself to a new host for a blood meal, it can transmit the spirochetes into the host's bloodstream. This transmission mechanism is essential for the maintenance of the bacteria in nature and the perpetuation of the disease cycle.

Host Range and Associated Diseases

The complex's extensive host range is a fascinating aspect of Borrelia burgdorferi sensu lato. It can infect a variety of hosts, including rodents, birds, and larger mammals. Different genospecies within the complex are often associated with specific host species and particular geographical regions. For instance, Borrelia afzelii is commonly found in rodents, particularly in Europe, while Borrelia garinii is associated with avian hosts.

In humans, Borrelia burgdorferi sensu lato is responsible for causing Lyme disease, also known as Lyme borreliosis. This disease is a multisystemic illness that can manifest with a wide range of symptoms. In its early stages, Lyme disease often presents with flu-like symptoms, including fever, fatigue, headache, muscle and joint aches, and swollen lymph nodes. One of the distinctive features of Lyme disease is the characteristic skin rash known as erythema migrans, which appears at the site of the tick bite.

If left untreated, the spirochetes can disseminate throughout the body, leading to more severe and diverse symptoms. Neurological complications, such as facial palsy and meningitis, can occur. Additionally, Lyme arthritis may develop, causing joint pain and inflammation, particularly in large joints like the knee. In rare cases, Lyme carditis can affect the heart's electrical conduction system, resulting in heart block.

Diagnostic Methods

Accurate diagnosis of Borrelia burgdorferi sensu lato infections, especially in the context of Lyme disease, is crucial for effective treatment. Several diagnostic methods are available:

1. Polymerase Chain Reaction (PCR): PCR is a molecular technique used to detect the presence of Borrelia DNA. Specific genes, often the 16S rRNA gene, are targeted, and through amplification, the pathogen's nucleic acids can be identified. PCR is particularly valuable in early-stage infections when the spirochetes may be present in low numbers.

2. Serological Tests: Enzyme immunoassays (such as ELISA) and Western blot assays are commonly employed to detect antibodies generated by the host against Borrelia antigens. Notable proteins used in these assays include Outer Surface Protein A (OspA) and Outer Surface Protein C (OspC). Serological tests are often used to confirm the diagnosis of Lyme disease and can be useful in later stages of the disease when antibody levels are higher.

Conclusion

Borrelia burgdorferi sensu lato, a complex of spirochetal bacteria, is a versatile and medically significant pathogen known for causing Lyme disease. Its unique structure, diverse genospecies, and the range of hosts it infects contribute to its complexity. Effective diagnosis through molecular and serological methods is critical for identifying and managing Lyme disease, which, if left untreated, can lead to a range of debilitating symptoms. As our understanding of Borrelia burgdorferi sensu lato continues to evolve, we gain new insights into the intricate interplay between the pathogen, its hosts, and the environment, which is essential for devising strategies for prevention and treatment.

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