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Catalot Number Size
(T,Test, formalized in 96 well, one test per well)
Price(In USD) Qty (Quantity) Sum(In USD)
GM-2019nCoV-PSV14-2 100T 3840
GM-2019nCoV-PSV14-2 200T 5440
GM-2019nCoV-PSV14-2 1000T 21760
GM-2019nCoV-PSV14-2 5000T 87040
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Shipping Cost: 760.00
Total:



GeneMedi Spike P2& S1/S2 cleavage site (furin cleavage sequence) mutant SARS-CoV-2(2019nCoV) Pseudotyped virus production and Pseudovirus Based Neutralization Assay.

The Spike protein (S-protein) of SARS-CoV-2 (2019nCoV) mediates receptor (ACE2) binding and cell entry and is the dominant target of the immune system. The highly flexible Spike protein (S-protein), with its mobile domains, transitions from closed to open conformations to expose its receptor-binding site and, subsequently, from prefusion to postfusion conformations to mediate fusion P2-mutated Spike protein trimer variant vector (P2-mutant,S1/S2 cleavage site(furin cleavage sequence)-mutant, trimerization modified)

The Spike protein (S-protein) of SARS-CoV-2 (2019nCoV) mediates receptor (ACE2) binding and cell entry and is the dominant target of the immune system. The highly flexible Spike protein (S-protein), with its mobile domains, transitions from closed to open conformations to expose its receptor-binding site and, subsequently, from prefusion to postfusion conformations to mediate fusion of viral and cellular membranes. SARS-CoV-2 (2019nCoV) Spike protein (S-protein) derivatives are components of vaccine candidates and diagnostic assays, as well as tools for research into the biology and immunology of SARS-CoV-2.

We designed Spike mutations that allow the production of thermostable, disulfide-bonded Spike-protein trimers that are trapped in the closed, prefusion state. Structures of the disulfide-stabilized and non-disulfide-stabilized proteins reveal distinct closed and locked conformations of the Spike protein trimer.

The thermostable, disulfide-bonded Spike-protein trimers construct we make is:
1) S1/S2 cleavage site (furin cleavage sequence) mutant: Modification of the coding sequence of the multibasic S1/S2 cleavage site (furin cleavage sequence) PRRAR to PGSAS at residues 682–685.
2) P2 mutant: proline substitutions at residues 986 and 987.
3) Trimerization statues: add exogenous trimerization signal peptides.

It has been demonstrated that the designed, thermostable, closed Spike trimer can be used in serological assays in COVID-19 test. This trimer protein has potential applications as a reagent for serology, virology and as an immunogen of COVID-19 related diagnostics and vaccine development.

GeneMedi offers pre-made gene ORF mutation plasmids of Spike trimer mutation(thermostable, disulfide-bonded Spike-protein trimers).
The pre-made gene ORF vector of Spike trimer mutation is coden optimized for mamamlian and can be used for mamalian expression.
GeneMedi also provides pre-made the lentivirus, adenovirus and AAV vector for the gene ORF plasmids of 2019 nCoV (SARS2 coronavirus).


Description



The outbreak of COVID-19, caused by SARS-CoV-2 (2019-nCoV), has been a global public health threat and caught the worldwide concern. GeneMedi has developed SARS-CoV-2 wildtype and mutation variants pseudovirus production system, from which the SARS-CoV-2 wildtype and mutation variants pseudotyped virus can be handled in biosafety level 2 (BSL-2).

GeneMedi develop COVID-19 related SARS-CoV-2 (2019-nCoV)Pseudotyped virus of SARS-CoV-2 Spike Mutation Variants including D614G,S943P,V367F, G476S, V483A, H49Y, Q239K, A831V, P1263L,D839Y/N/E:D839Y,D839N,D839E,P2-mutated Spike protein trimer variant (P2-mutant, S1/S2 cleavage site(furin cleavage sequence)-mutant, trimerization modified), Spike(S1+S2) - B.1.1.7 lineage, Spike(S1+S2)-N501Y mutation, Spike(S1+S2)-HV 69-70 Deletion mutation(ΔH69/ΔV70) and so on.

SARS-CoV-2 Pseudotyped virus with Spike D614G mutation:Increasing frequency and global distribution.
SARS-CoV-2 Pseudotyped virus with Spike S943P mutation:It is found only in Belgium
SARS-CoV-2 Pseudotyped virus with Spike V367F mutation:
SARS-CoV-2 Pseudotyped virus with Spike G476S mutation
SARS-CoV-2 Pseudotyped virus with Spike V483A mutation
SARS-CoV-2 Pseudotyped virus with Spike H49Y mutation
SARS-CoV-2 Pseudotyped virus with Spike Q239K mutation
SARS-CoV-2 Pseudotyped virus with Spike A831V mutation
SARS-CoV-2 Pseudotyped virus with Spike P1263L mutation
SARS-CoV-2 Pseudotyped virus with Spike D839Y/N/E-D839Y mutation
SARS-CoV-2 Pseudotyped virus with Spike D839Y/N/E-D839N mutation
SARS-CoV-2 Pseudotyped virus with Spike D839Y/N/E-D839E mutation
SARS-CoV-2 Pseudotyped virus with P2-mutated Spike protein trimer variant (P2-mutant, S1/S2 cleavage site (furin cleavage sequence)-mutant,trimerization modified) mutation
SARS-CoV-2 Pseudotyped virus with Spike of SARS-COV-2 B.1.1.7 lineage mutation
SARS-CoV-2 Pseudotyped virus with Spike(S1+S2) N501Y mutation mutation
SARS-CoV-2 Pseudotyped virus with Spike(S1+S2)-HV 69-70 Deletion mutation(ΔH69/ΔV70) mutation

GeneMedi’s Pseudovirus Based Neutralization Assay (PBNA) is a conventional assay method that is suitable for High-Throughout Screening (HTS) without live virus engaged. The Pseudovirus Based Neutralization Assay can be used for evaluating:

1) Neutralizing antibodies
2) Peptides blockers (peptide inhibitors)
3) Types of Vaccines3
4) Compounds targeting Spike induced cell-fusion.

GeneMedi offers:

1.SARS-CoV-2 Pseudotyped virus packaging and production
2.Effector cells: human ACE2 overexpression stable HEK293T cell lines
3.SARS-CoV-2(2019nCoV) Pseudotyped Virus Based Neutralization Assay service.