Recombinant multi-species AKT1/ AKT/ CWS6 protein for cell curture, in vitro study, in vivo study, benchmark, drug discovery & MOA research and positive control for the diagnosis

Genemedi produces recombinant Human, Cynomolgus/ Rhesus macaque, Rat, Mouse, Feline, Canine, Bovine, Equine AKT1 protein for cell curture, in vitro study, in vivo study, benchmark, drug discovery & MOA research and positive control for the diagnosis.

The target: AKT1, gene name: AKT1, also named as AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA. This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020].

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Catalog No.	Product Name	Species	Size	Price
GM-Tg-hg-T67619-Ag-1	Human AKT1 protein	Human	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-rg-T67619-Ag-1	Rat AKT1 protein	Rat	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-mg-T67619-Ag-1	Mouse AKT1 protein	Mouse	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-cynog-T67619-Ag-1	Cynomolgus/ Rhesus macaque AKT1 protein	Cynomolgus/ Rhesus macaque	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-felg-T67619-Ag-1	Feline AKT1 protein	Feline	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-cang-T67619-Ag-1	Canine AKT1 protein	Canine	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-bovg-T67619-Ag-1	Bovine AKT1 protein	Bovine	1mg/10mg/100mg/≥100mg	Inquiry
GM-Tg-bovg-T67619-Ag-1	Equine AKT1 protein	Equine	1mg/10mg/100mg/≥100mg	Inquiry
Shipping Cost:				760.00

Description

Catalog No.	GM-Tg-hg-T67619-Ag, GM-Tg-rg-T67619-Ag, GM-Tg-mg-T67619-Ag, GM-Tg-cynog-T67619-Ag, GM-Tg-felg-T67619-Ag, GM-Tg-cang-T67619-Ag, GM-Tg-bovg-T67619-Ag, GM-Tg-equg-T67619-Ag-1
Products Name	AKT1 protein
Species	Human, Cynomolgus/ Rhesus macaque, rat, mouse, Feline, Canine, Bovine, Equine
Target Name	AKT1
Protein Sub-location	Introcelluar Protein
Isotypes	Recombinant protein
Expression platform	Mammalian cell
Bioactivity validation	Affintiy&bioactivity validated by ELISA, cell culture validated.
Tag	His
Products description	Recombinant Human, Cynomolgus/ Rhesus macaque, Rat, Mouse, Feline, Canine, Bovine, Equine AKT1 protein was expressed in mammalian cell expression system and is expressed with 6 HIS tag at the C-terminus for cell culture, ELISA or other affinity binding assay or functional assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic).
Purity	Purity: ≥95% (SDS-PAGE)
Application	In vitro study, in vivo study, benchmark, positive control for the diagnosis. Biological drug disovery including cell culture, assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic) and mechanism of action (MOA) research.
Formulation	Supplied as a 0.2 μM filtered solution of PBS, PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.