Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) antibody and antigen (recombinant protein)

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Diagnostic anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) antibodies pairs and antigen for animal health (animal Avian/Bird/Poultry infectious disease Avian mycoplasmosis(Mycoplasma gallisepticum infection)) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P177-Tg003-Ag01	Recombinant Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) protein	$3090.00
GMP-VT-P177-Tg003-Ab01	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P177-Tg003-Ab02	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P177-Tg003-Ab03	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) human monoclonal antibody (mAb)	$3090.00
GMP-VT-P177-Tg003-Ab04	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) human monoclonal antibody (mAb)	$3090.00

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Product Description

Cat No.	GMP-VT-P177-Tg003-Ag01
Product Name	Recombinant Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) protein
Pathogen	Mycoplasma gallisepticum
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Mycoplasma gallisepticum level test of animal Avian/Bird/Poultry infectious disease with Avian mycoplasmosis(Mycoplasma gallisepticum infection).
Tag	His
Product description	Recombinant Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P177-Tg003-Ab01,GMP-VT-P177-Tg003-Ab02
Pathogen	Mycoplasma gallisepticum
Product Name	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycoplasma gallisepticum antibodies in Mycoplasma gallisepticum level test of animal Avian/Bird/Poultry infectious disease with Avian mycoplasmosis(Mycoplasma gallisepticum infection).
Product description	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycoplasma gallisepticum antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P177-Tg003-Ab03,GMP-VT-P177-Tg003-Ab04
Pathogen	Mycoplasma gallisepticum
Product Name	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycoplasma gallisepticum antibodies in Mycoplasma gallisepticum level test of animal Avian/Bird/Poultry infectious disease with Avian mycoplasmosis(Mycoplasma gallisepticum infection).
Product description	Anti-Mycoplasma gallisepticum hemagglutinin protein encoded by pMGA1.2 (a member of the vlhA gene family) mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Mycoplasma gallisepticum (MG) is a type of prokaryotic bacterium that causes avian mycoplasmosis, also known as chronic respiratory disease (CRD), in poultry. This pathogen mainly infects birds, including chickens and turkeys, causing respiratory disease and decreased egg production. In this article, we will provide a detailed description of Mycoplasma gallisepticum, including its classification, structure, host range, and diagnostic methods.

Classification

Mycoplasma gallisepticum is a member of the class Mollicutes under the phylum Tenericutes. This class comprises a group of bacteria that are characterized by their lack of a cell wall and small genome size. Mycoplasma gallisepticum is a pleomorphic bacterium, meaning it can change its shape due to the absence of a cell wall.

Structure

Mycoplasma gallisepticum has a unique structure compared to other bacteria. As mentioned, it lacks a cell wall and therefore does not possess peptidoglycan. Instead, the bacterium has an S-layer that surrounds the cell membrane. The S-layer is composed of glycoproteins that allow the bacterium to attach to host cells and evade the host's immune system. In addition, Mycoplasma gallisepticum has a small genome, consisting of approximately 1.1 million base pairs.

Host Range and Diseases

Mycoplasma gallisepticum mainly infects birds, including chickens and turkeys, but it has also been reported in other avian species such as pheasants, quails, and ducks. The pathogen causes a range of diseases in infected hosts, with respiratory disease being the most common. CRD is a highly contagious respiratory disease that affects the upper respiratory tract of birds, leading to nasal discharge, conjunctivitis, wheezing, and coughing. In addition to respiratory disease, Mycoplasma gallisepticum also causes infectious sinusitis in poultry, which is characterized by inflammation of the sinuses and swelling of the eyes, leading to a decrease in egg production.

Diagnostic Methods

Several diagnostic methods have been developed for detecting Mycoplasma gallisepticum in infected birds. Culture-based methods involve isolating the bacterium from host samples, such as blood, nasal swabs, or tracheal swabs, and growing it in vitro. This method is time-consuming and requires specialized laboratory equipment. Serological tests detect antibodies against M. gallisepticum antigens in the host serum or plasma. This method is useful for detecting past exposure to the bacterium but is not suitable for diagnosing active infections.

Nucleic acid-based methods are the most commonly used diagnostic tests for Mycoplasma gallisepticum. Polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) are two nucleic acid-based methods that have been developed for detecting Mycoplasma gallisepticum in infected birds. These methods target specific genes or regions of the Mycoplasma gallisepticum genome, such as the 16S rRNA gene and the pvpA gene. PCR and LAMP are highly sensitive and specific, allowing for rapid and accurate diagnosis of Mycoplasma gallisepticum infections in birds.

Conclusion

In conclusion, Mycoplasma gallisepticum is a prokaryotic bacterium that causes avian mycoplasmosis, mainly affecting chickens and turkeys. This pathogen has a unique structure lacking a cell wall, and it causes respiratory disease and decreased egg production in infected birds. Various diagnostic methods have been developed for detecting Mycoplasma gallisepticum, including culture-based, serological, and nucleic acid-based methods. Nucleic acid-based methods are the most sensitive and specific diagnostic tests for Mycoplasma gallisepticum, allowing for rapid and accurate diagnosis of infected birds.

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