Clostridium botulinum type C and D HCC and HCD antibody and antigen (recombinant protein)

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Diagnostic anti-Clostridium botulinum type C and D HCC and HCD antibodies pairs and antigen for animal health (animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Avian/Bird/Poultry infectious disease botulism) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P266-Tg001-Ag01	Recombinant Clostridium botulinum type C and D HCC and HCD protein	$3090.00
GMP-VT-P266-Tg001-Ab01	Anti-Clostridium botulinum type C and D HCC and HCD mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P266-Tg001-Ab02	Anti-Clostridium botulinum type C and D HCC and HCD mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P266-Tg001-Ab03	Anti-Clostridium botulinum type C and D HCC and HCD human monoclonal antibody (mAb)	$3090.00
GMP-VT-P266-Tg001-Ab04	Anti-Clostridium botulinum type C and D HCC and HCD human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P266-Tg001-Ag01
Product Name	Recombinant Clostridium botulinum type C and D HCC and HCD protein
Pathogen	Clostridium botulinum type C and D
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Clostridium botulinum type C and D HCC and HCD antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Clostridium botulinum type C and D level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Avian/Bird/Poultry infectious disease with botulism.
Tag	His
Product description	Recombinant Clostridium botulinum type C and D HCC and HCD proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P266-Tg001-Ab01,GMP-VT-P266-Tg001-Ab02
Pathogen	Clostridium botulinum type C and D
Product Name	Anti-Clostridium botulinum type C and D HCC and HCD mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Clostridium botulinum type C and D HCC and HCD antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium botulinum type C and D antibodies in Clostridium botulinum type C and D level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Avian/Bird/Poultry infectious disease with botulism.
Product description	Anti-Clostridium botulinum type C and D HCC and HCD mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium botulinum type C and D antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P266-Tg001-Ab03,GMP-VT-P266-Tg001-Ab04
Pathogen	Clostridium botulinum type C and D
Product Name	Anti-Clostridium botulinum type C and D HCC and HCD human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Clostridium botulinum type C and D HCC and HCD antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium botulinum type C and D antibodies in Clostridium botulinum type C and D level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Avian/Bird/Poultry infectious disease with botulism.
Product description	Anti-Clostridium botulinum type C and D HCC and HCD mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Clostridium botulinum type C and D are two subtypes of the pathogenic bacterium Clostridium botulinum. This bacterium is a Gram-positive, obligate anaerobic prokaryote that can produce a potent neurotoxin called botulinum neurotoxin (BoNT). The neurotoxin is made up of two proteins: a heavy chain and a light chain. The heavy chain consists of two subunits, HC1 and HC2, which facilitate the entry of the toxin into nerve cells, while the light chain acts as a protease that cleaves the SNARE proteins involved in neurotransmitter release, leading to flaccid paralysis. BoNT is considered one of the most toxic substances known to man, and even small doses can lead to serious illness or death.

C. botulinum has a characteristic rod-shaped morphology and is spore-forming, meaning that it can form highly resistant endospores under unfavorable conditions. These endospores can survive harsh environmental conditions, such as high temperatures and low pH, making them difficult to eliminate. The bacterium typically grows in anaerobic environments, such as soil, water, and decaying organic matter. It can also grow in improperly canned or preserved foods, where it can produce BoNT, leading to foodborne botulism.

C. botulinum serotypes C and D can infect a variety of hosts, including humans, birds, and mammals. The diseases caused by this pathogen include botulism, a severe form of food poisoning, and also a variety of illnesses in animals such as avian botulism and botulism in cattle and horses. In humans, botulism is characterized by muscular weakness and paralysis, and can be fatal if left untreated. Symptoms typically occur within 6-36 hours after ingestion of contaminated food or wound infection, and may include blurred vision, dry mouth, difficulty speaking or swallowing, and muscle weakness.

To diagnose botulism, several methods are available. The mouse bioassay is considered the gold standard for detecting botulinum toxin in serum or food samples. This method involves injecting a small amount of the patient's serum or suspected food sample into mice and observing them for signs of botulism. However, this method is time-consuming and requires specialized equipment and facilities. Other detection methods include ELISA for detecting the BoNT protein, and mass spectrometry for detecting specific toxins.

More recently, nucleic acid-based methods have also been developed for rapid and sensitive detection of Clostridium botulinum, including PCR and next-generation sequencing. These methods target specific genes or proteins, such as the bont gene or BoNT protein, allowing for rapid and accurate detection of the pathogen in clinical or environmental samples. PCR is particularly useful, as it allows for the simultaneous detection of multiple serotypes of C. botulinum, and can distinguish between toxigenic and non-toxigenic strains.

In conclusion, Clostridium botulinum type C and D are two dangerous subtypes of C. botulinum, capable of producing a potent neurotoxin that causes severe foodborne illness and animal botulism. The bacterium is highly resistant to environmental stressors, making it difficult to eliminate from food or other environments. While traditional diagnostic methods such as the mouse bioassay and ELISA are still widely used, nucleic acid-based methods such as PCR have revolutionized the field of clostridial detection, allowing for faster and more accurate diagnosis of this deadly pathogen.

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