Escherichia coli F5 K99 antibody and antigen (recombinant protein)

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Diagnostic anti-Escherichia coli F5 K99 antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease diarrhea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P284-Tg002-Ag01	Recombinant Escherichia coli F5 K99 protein	$3090.00
GMP-VT-P284-Tg002-Ab01	Anti-Escherichia coli F5 K99 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P284-Tg002-Ab02	Anti-Escherichia coli F5 K99 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P284-Tg002-Ab03	Anti-Escherichia coli F5 K99 human monoclonal antibody (mAb)	$3090.00
GMP-VT-P284-Tg002-Ab04	Anti-Escherichia coli F5 K99 human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P284-Tg002-Ag01
Product Name	Recombinant Escherichia coli F5 K99 protein
Pathogen	Escherichia coli F5
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Escherichia coli F5 K99 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Escherichia coli F5 level test of animal Swine/Porcine/Pig infectious disease with diarrhea.
Tag	His
Product description	Recombinant Escherichia coli F5 K99 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P284-Tg002-Ab01,GMP-VT-P284-Tg002-Ab02
Pathogen	Escherichia coli F5
Product Name	Anti-Escherichia coli F5 K99 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Escherichia coli F5 K99 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli F5 antibodies in Escherichia coli F5 level test of animal Swine/Porcine/Pig infectious disease with diarrhea.
Product description	Anti-Escherichia coli F5 K99 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli F5 antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P284-Tg002-Ab03,GMP-VT-P284-Tg002-Ab04
Pathogen	Escherichia coli F5
Product Name	Anti-Escherichia coli F5 K99 human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Escherichia coli F5 K99 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli F5 antibodies in Escherichia coli F5 level test of animal Swine/Porcine/Pig infectious disease with diarrhea.
Product description	Anti-Escherichia coli F5 K99 mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Escherichia coli (E. coli) is a gram-negative bacterium that is commonly found in the gut of humans and animals. Most strains of E. coli are harmless and even beneficial to the host, as they help in the production of vitamins and prevent colonization by pathogenic bacteria. However, certain strains of E. coli can cause serious diseases in humans and animals, including E. coli F5.

Escherichia coli F5, also known as STEC F5, is a subset of Shiga toxin-producing Escherichia coli (STEC) that produce Shiga toxins associated with severe disease symptoms. These toxins can cause damage to endothelial cells in the body's blood vessels, causing microangiopathic hemolytic anemia and thrombotic microangiopathy. In addition, they can cause acute renal failure and neurological complications.

The genome of Escherichia coli F5 consists of a single circular chromosome and several plasmids. The bacterium has evolved a range of virulence factors that enable it to colonize host tissues and evade host immune responses. One of the most critical virulence factors is the Shiga toxin, which is encoded by the stx gene located on a bacteriophage within the genome. This toxin inhibits protein synthesis in eukaryotic cells and causes death in infected cells.

Escherichia coli F5 primarily infects ruminants such as cattle, goats, and sheep, but it can also infect humans. Humans typically contract E. coli F5 infections through contact with infected animal feces or meat contaminated during processing. The pathogen can cause multiple diseases in humans, including mild diarrhea, hemorrhagic colitis, and HUS.

Hemorrhagic colitis is characterized by bloody diarrhea and abdominal cramps, while HUS is characterized by hemolytic anemia, thrombocytopenia, and acute renal failure. HUS is a potentially life-threatening complication of E. coli F5 infection, particularly in children and the elderly.

Diagnosis of E. coli F5 infection typically involves a combination of clinical symptoms and laboratory tests. Clinical symptoms include abdominal pain, diarrhea, and blood in the stool. Laboratory tests include bacterial culture from fecal samples, which can identify the presence of E. coli F5 in the gut. Serological tests for antibody detection can also be used to confirm exposure to the pathogen, and nucleic acid-based assays such as PCR can detect bacterial DNA in clinical samples with high specificity and sensitivity. PCR-based assays target various genes associated with virulence and pathogenicity, including stx, eae, and hlyA, among others.

Treatment of E. coli F5 infection typically involves supportive care and antibiotics for secondary infections. Severe cases of HUS may require hospitalization and intensive care, including dialysis, plasma exchange, and blood transfusions. Preventative measures for E. coli F5 include proper food handling and cooking, handwashing, and avoidance of contact with infected animals or their feces.

In conclusion, Escherichia coli F5 is a dangerous pathogen that can cause severe diseases such as hemorrhagic colitis and HUS in humans. The primary virulence factor of E. coli F5 is the Shiga toxin, which inhibits protein synthesis in eukaryotic cells and causes cell death. Diagnosis of the pathogen involves a combination of clinical symptoms and laboratory tests, including bacterial culture, serological tests, and nucleic acid-based assays. Treatment of E. coli F5 infection typically involves supportive care and antibiotics, and prevention includes proper food handling, handwashing, and avoidance of contaminated items.

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