Diagnostic Phospho-tau217 (p-tau217) antibodies (anti- Phospho-tau217, anti-p-tau217) for Neuropathy p-tau217 detection in ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA),immunonephelometry and POCT.

**For the latest product details, please Click Here.

Database Download

Alzheimer Disease(AD) Recent publications and meeting presentations bolster the evidence that upticks in p-tau181, p-tau217, and p-tau231 occur soon after Aβ begins to accumulate in the brain. That’s years before neurofibrillary tangles can be detected by PET, and decades before symptoms. Researchers predict these markers, which also distinguish AD from other forms of dementia, will lead to more robust tests than those based on Aβ, because of their larger dynamic range—phosphorylated fragments of tau climb by as much as 10-fold in the blood as AD progresses, whereas the Aβ42/40 ratio falls by about 15 percent at most.

Genemedi produces core diagnostic ingredients for test of Neuropathy and related syndrome. GeneMedi offers paired diagnostics grade Phospho-tau217 (p-tau217) antibodies (monoclonal antibody,mab)and Phospho-tau217 (p-tau217) antigens for Neuropathy rapid test kit of Phospho-tau217 (p-tau217) testing in ELISA test,Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA),immunonephelometry and POCT.

All the antibodies and antigens for Neuropathy test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibodies can act as a capture antibody and detection antibody.The antigens can be used for antibodies rapid test of Neuropathy.

Order information

Catalog No.	Size	Price(In USD)	Qty (Quantity)	Sum(In USD)
GMP-p-tau217-Ab-1	Size:1mg	1953
GMP-p-tau217-Ab-1	Size:10mg	13671
GMP-p-tau217-Ab-1	Size:100mg	95697
GMP-p-tau217-Ab-2	Size:1mg	1953
GMP-p-tau217-Ab-2	Size:10mg	13671
GMP-p-tau217-Ab-2	Size:100mg	95697
GMP-p-tau217-Ab	≥100mg	Inquiry
			Shipping Cost:	760.00
			Total:

For more than 100mg, click here for inquiry. >>

Description

Cat No.	GMP-p-tau217-Ab-1; GMP-p-tau217-Ab-2
Product Name	Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody(mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Human Phospho-tau217 (p-tau217) protein binding, ELISA validated as capture antibody. Pair in p-tau217level test and Neuropathy evaluation.
Tag	mFc
Products description	Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody(mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody pair.
Specics	mouse
Purity	Purity: ≥95% (SDS-PAGE)
Application	ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Validation Data

Good performance of GeneMedi's p-tau/tau antibodies in ELISA Validation

1. GeneMedi's three phosphorylated tau protein antibodies (p-tau181, p-tau217, and p-tau231) demonstrate outstanding sensitivity and exceptional specificity with their corresponding phosphorylated forms in direct ELISA assays.

GeneMedi's p-tau antibodies and antigens demonstrate excellent binding affinity: GMP-h-p-tau181-Ab01 effectively binds with GMP-h-p-tau181-Ag01, GMP-h-p-tau217-Ab01 binds well with GMP-h-p-tau217-Ag01, and GMP-h-p-tau231-Ab01 shows strong binding with GMP-h-p-tau231-Ag01. These results indicate that GeneMedi's p-tau antibodies and antigens possess outstanding affinity.
Product used as below:

Cat NO.	Description
GMP-h-p-tau181-Ab01	Anti-Phospho-tau181 (p-tau181) mouse monoclonal antibody (mAb)
GMP-h-p-tau181-Ag01	Phospho-tau181 (p-tau181) antigen (peptide)
GMP-h-p-tau217-Ab01	Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)
GMP-h-p-tau217-Ag01	Phospho-tau217 (p-tau217) antigen (peptide)
GMP-h-tau231-Ab01	Anti-Phospho-tau231 (p-tau231) mouse monoclonal antibody (mAb)
GMP-h-p-tau231-Ag01	Phospho-tau231 (p-tau231) antigen (peptide)

Fig 1. Validation of GeneMedi's Anti-human p-tau181/p-tau217/p-tau231 antibody in direct ELISA.

A. GeneMedi's GMP-h-p-tau181-Ab01 (Anti-human p-tau181 antibody) is validated to detect the GMP-h-p-tau181-Ag01 (p-tau181 antigen (peptide)) in ELISA, EC50 = 25.26 ng/ml.
B. GeneMedi's GMP-h-p-tau217-Ab01 (Anti-human p-tau217 antibody) is validated to detect the GMP-h-p-tau217-Ag01 (p-tau217 antigen (peptide)) in ELISA, EC50 = 25.85 ng/ml.
C. GeneMedi's GMP-h-p-tau231-Ab01 (Anti-human p-tau231 antibody) is validated to detect the GMP-h-p-tau231-Ag01 (p-tau231 antigen (peptide)) in ELISA, EC50 = 64.34 ng/ml.
The total tau antibody (GMP-h-TauAb01) does not interact with the p-tau181 (GMP-h-p-tau181-Ag01), p-tau217 (GMP-h-p-tau217-Ag01), and p-tau231 (GMP-h-p-tau231-Ag01) peptide antigen. (Data not shown)

2. In direct ELISA assays, GeneMedi's phosphorylated tau217 antibody demonstrates excellent sensitivity and precision for both full-length and peptide forms of the phosphorylated tau217 antigen. However, its ability to detect other phosphorylated antigens and total tau antigen is limited, indicating its highly specificity.

GeneMedi's GMP-h-p-tau217-Ab01 antibody demonstrates excellent binding ability with the full-length antigen GMP-h-p-tau217-Ag02 as well as the peptide GMP-h-p-tau217-Ag01. However, when interacting with the antigens GMP-h-p-tau181-Ag01, GMP-h-p-tau231-Ag01, and GMP-h-Tau-Ag01, the affinity is relatively weaker, as reflected by significant differences in EC50 values. These results indicate that GeneMedi's p-tau 217 antibody exhibits high affinity and specificity.
Product used as below:

Cat NO.	Description
GMP-h-p-tau181-Ag01	Phospho-tau181(p-tau181) antigen (peptide)
GMP-h-p-tau217-Ag01	Phospho-tau217 (p-tau217) antigen (peptide)
GMP-h-p-tau231-Ag01	Phospho-tau231 (p-tau231) antigen (peptide)
GMP-h-p-tau217-Ag02	Phospho-tau217 (p-tau217) antigen (full length)
GMP-h-Tau-Ag01	Recombinant human MAPT/Tau/MAPTL/MTBT1 Protein
GMP-h-p-tau217-Ab01	Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)

Fig 2. Direct ELISA Binding Curves of Phosphorylated Tau217 Antibody with Various Antigens.

This graph displays the binding efficiency of the phosphorylated tau217 antibody with five different antigens in an ELISA setup. The x-axis represents the antibody concentration in a logarithmic scale (μg/ml), and the y-axis indicates the corrected absorbance, reflecting the binding efficiency between the antibody and antigens. The data illustrate that GMP-h-p-tau217-Ab01 exhibits high detection efficiency for GMP-h-p-tau217-Ag01 (peptide) with an EC50 of 4.326 ng/ml. For GMP-h-p-tau217-Ag02 (full length), the antibody demonstrates exceptionally high sensitivity, with an EC50 of only 6.943 ng/ml. Additionally, this antibody effectively detects GMP-h-p-tau181-Ag01 (peptide), GMP-h-p-tau231-Ag01 (peptide) and GMP-h-tau-Ag01 (full length) with EC50 values of 27320 ng/ml, 3563 ng/ml and 1843 ng/ml.

3. In sandwich ELISA assays, GeneMedi's p-tau217 antibody and tau antibody pair can detect the full-length phosphorylated 217 protein but are unable to detect phosphorylated 217 peptides or non-phosphorylated proteins.

Cat NO.	Description
GMP-h-p-tau217 Ag01	Phospho-tau217 (p-tau217) antigen (peptide)
GMP-h-p-tau217 Ag02	Phospho-tau217 (p-tau217) antigen (full length)
GMP-h-p-tau217 Ab01	Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)
GMP-h-p-tau217 Ab02	Anti-Phospho-tau217 (p-tau217) mouse monoclonal antibody (mAb)
GMP-h-tau-Ag01	Recombinant human MAPT/Tau/MA PTL/MTBT1 Protein
GMP-h-tauAb01	A nti-human MAPT/Tau/MA PTL/MTBT1 mouse monoclonal antibody (mAb)
GMP-h-tau-Ab02	Anti-human MAPTITau/MA PTL/MTBT1 mouse monoclonal antibody (mAb)
GMP-h-tau-Ab03	Anti-human MAPTITau/MA PTL/MTBT1 mouse monoclonal antibody (mAb)

GMP-h-p-tau217-Ab01 is used as the capture antibody. When GMP-h-Tau-Ab03 is used as the detection antibody, the EC50 for detecting GMP-h-p-tau217-Ag02 is lower than GMP-h-Tau-Ab01, indicating GMP-h-Tau-Ab03 is more suitable as a detection antibody.
However, when detecting phosphorylated 217 peptide GMP-h-p-tau217-Ag01 or non-phosphorylated protein GMP-h-tau-Ag01, the EC50 remains high regardless of whether GMP-h-Tau-Ab03 or GMP-h-Tau-Ab01 is used, suggesting no binding for detecting peptide GMP-h-p-tau217-Ag01 or non-phosphorylated protein GMP-h-tau-Ag01 under these conditions.

Fig3. Sandwich ELISA Analysis of Full-Length Phosphorylated Tau 217 Antigen with Corresponding Phosphorylated Tau 217 Antibody and Total Tau Antibody.

A.This figure illustrates the detection efficacy of two different antibody combinations in Sandwich ELISA experiments targeting the GMP-h-p-tau217-Ag02 antigen (full length). The x-axis represents the antigen concentration displayed on a logarithmic scale (μg/ml), while the y-axis shows the corrected absorbance. The results indicate that the combination of GMP-h-p-tau217-Ab01 as the coating antibody and GMP-h-Tau-Ab01 as the detection antibody yields an EC50 of 76.55 ng/ml. Conversely, using GMP-h-p-tau217-Ab01 as the coating antibody and GMP-h-Tau-Ab03 as the detection antibody results in an EC50 of 39.02 ng/ml, demonstrating higher detection sensitivity at lower concentrations.
B.This figure demonstrates the detection results of two different detection antibodies (GMP-h-tau-Ab01 and GMP-h-tau-Ab03) against the GMP-h-tau-Ag01 antigen (full length) in an ELISA assay, using GMP-h-p-tau217-Ab01 as the coating antibody. The x-axis represents antigen concentration, displayed on a logarithmic scale (μg/ml); the y-axis shows corrected absorbance. The results reveal that when using GMP-h-tau-Ab03 as the detection antibody, the EC50 value reaches 20030 ng/ml, indicating a lower sensitivity to the antigen; whereas, when using GMP-h-tau-Ab01 as the detection antibody, the EC50 value soars to 4352000 ng/ml, demonstrating poor efficacy in detecting the antigen.
C. This figure illustrates the detection performance of four different antibody combinations in an ELISA assay targeting the GMP-h-p-tau217-Ag01 antigen (peptide). The x-axis represents antigen concentration, displayed on a logarithmic scale (μg/ml), while the y-axis shows the corrected absorbance. The curves in the figure show that all antibody combinations maintain relatively consistent absorbance changes across a range of low to high concentrations, indicating these results also reveal that the sensitivity of these four antibody combinations in detecting the GMP-h-p-tau217-Ag01 (peptide) antigen is relatively low.

4. GeneMedi's Tau Protein antibodies exhibit remarkable sensitivity and exceptional specificity when tested with GeneMedi’s recombinant human Tau Protein in direct ELISA assays.

Fig4. Validation of GeneMedi's Anti-human Tau antibody.
GeneMedi's GMP-h-Tau-Ab01/03/05/06/07/08/09/10/11/12 (Anti-human Tau antibody) is validated to detect the GMP-h-Tau-Ag01 (Recombinant human Tau Protein) in ELISA.

5. In sandwich ELISA assays, GeneMedi's p-tau217/tau antibody pairs can detect the full-length phosphorylated 217 protein but are unable to detect the non-phosphorylated full-length Tau protein

Fig5. Sandwich ELISA Analysis of Full-Length Phosphorylated Tau 217 Antigen with Corresponding Phosphorylated Tau 217 Antibody and Total Tau Antibody.
These figures illustrate the detection efficacy of different antibody combinations in Sandwich ELISA experiments targeting the GMP-h-p-tau217-Ag02 antigen (full length). The results show that when using GMP-h-Tau-Ab01 (A), GMP-h-Tau-Ab03 (B), GMP-h-Tau-Ab05 (C), and GMP-h-Tau-Ab06 (D), GMP-h-Tau-Ab07 (E), GMP-h-Tau-Ab08 (F), GMP-h-Tau-Ab09 (G), GMP-h-Tau-Ab10 (H), GMP-h-Tau-Ab11 (I) and GMP-h-Tau-Ab12 (J) respectively as the detection antibodies, and GMP-h-p-tau217-Ab01/03/05/06 as the coating antibody in each case. As a negative control, when the same antibody pairs target the GMP-h-Tau-Ag01 antigen (full length), the resulting EC50 is NA, which demonstrates their high specificity and sensitivity.
The table shows the EC50 values of different antibody combinations in sandwich ELISA experiments targeting the GMP-h-p-tau217-Ag02 antigen (full-length), as shown in A–J. GMP-h-p-Tau217-Ab06 shows the best EC50 in almost all antibody pairs.

6. Validation of High-Specificity p-Tau217/181/231 and Tau Antibody Pairs

The ELISA validation data demonstrates the exceptional specificity of p-Tau217/181/231 and tau antibody pairs: p-Tau217/Tau pair exclusively recognizes p-Tau217 full-length antigen (Figure A); p-Tau181/Tau pair shows selective binding to p-Tau181 full-length antigen (Figure B); p-Tau231/Tau pair specifically detects p-Tau231 full-length antigen (Figure C). The complete absence of cross-reactivity with non-target phospho-Tau isoforms confirms the superior specificity of these antibody pairs, making them ideal for precise Alzheimer's disease biomarker detection.

Fig6. High specificity and reliability of p-Tau217/181/231 and tau antibody pairs In ELISA.
A. p-Tau217/ tau antibody pair binds specifically to p-Tau217 full-length antigen.
B. p-Tau181/ tau antibody pair binds specifically to p-Tau181 full-length antigen.
C. p-Tau231/ tau antibody pair binds specifically to p-Tau231 full-length antigen.

7. High specificity and reliability of pTau217/181/231 Phosphorylated full-length protein

GeneMedi is proud to offer pTau217/181/231 phosphorylated full-length protein. As shown in Figures A, B, and C: p-Tau217 full-length antigen specifically binds to p-Tau217 antibodies (Figure A); p-Tau181 full-length antigen specifically binds to p-Tau181 antibodies (Figure B); p-Tau231 full-length antigen specifically binds to p-Tau231 antibodies (Figure C). These results demonstrate that pTau217/181/231 phosphorylated full-length antigens are phosphorylated at their respective sites, with minimal phosphorylation at other sites, ensuring high specificity and reliability for research and diagnostic applications.

Fig7. High specificity and reliability of p-Tau217/181/231 Phosphorylated Full-Length Antigens In ELISA.
A. p-Tau217 full-length antigen binds specifically to p-Tau217 antibodies.
B. p-Tau181 full-length antigen binds specifically to p-Tau181 antibodies.
C. p-Tau231 full-length antigen binds specifically to p-Tau231 antibodies.