AAV Vector Transduction-AAV vector Gene Delivery in vivo

Adeno Associated Virus (AAV) For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is shown in Table5.

Table5. AAV gene delivery methods in specific organs.
Infection organ	Recommended serotype	Injection route	Animal	Injection volume (μl)
Heart	AAV9	Multiple points in situ	Rat	10-15/point, 3-5 points
		Multiple points in situ	Mouse	10-15/point, 3-5 points
		Tail vein	Rat	250 (200g body weight)
		Tail vein	Mouse	100
Liver	AAV8 or AAV9	Tail vein	Rat	200 (200g body weight)
Liver	AAV8 or AAV9	Tail vein	Mouse	100
Whole brain	AAV-PHP.eB AAV-PHP.B	Tail vein	Rat	200 (200g body weight)
Whole brain	AAV-PHP.eB AAV-PHP.B	Tail vein	Mouse	100
Lateral ventricle	AAV9	Stereotactic	Rat	1-5
Lateral ventricle	AAV9	Stereotactic	Mouse	1-5
Brain tissue	AAV9	Stereotactic	Rat	2-3
Brain tissue	AAV9	Stereotactic	Mouse	1-2
Fat	AAV9	Intraperitoneal injection for intraperitoneal fat	Rat	300
		Intraperitoneal injection for intraperitoneal fat	Mouse	150-200
		in situ injection for subcutaneous fat	Rat	10-15/point, 3-5 points
		in situ injection for subcutaneous fat	Mouse	10-15/point, 3-5 points
Skeletal muscle	AAV1 or AAV9	In situ injection	Rat	10-15/point, 3-5 points
Skeletal muscle	AAV1 or AAV9	In situ injection	Mouse	10-15/point, 3-5 points
Eyes	AAV2, AAV10 or AAV-DJ	vitreous chamber injection	Rat	3-5
		vitreous chamber injection	Mouse	1-3
		subretinal space injection	Rat	1-2
		subretinal space injection	Mouse	1-2
Lung	AAV6	Intratracheal injection	Rat	100 (200g weight)
Lung	AAV6	Intratracheal injection	Mouse	50-75
Kidney	AAV2 or AAV9	Renal pelvis injection	Rat	10-15/point, 3-5 points
Kidney	AAV2 or AAV9	Renal pelvis injection	Mouse	10-15/point, 3-5 points
Intestine	AAV1 or AAV5	Enema	Rat	200 (200g weight)
Intestine	AAV1 or AAV5	Enema	Mouse	100

Detailed information of AAV in vivo animal injection protocol can be seen in AAV User Manual.

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