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Adeno Associated Virus (AAV)

For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is shown in Table5.

Table5. AAV gene delivery methods in specific organs.
Infection organ Recommended serotype Injection route Animal Injection volume (μl)
Heart AAV9 Multiple points in situ Rat 10-15/point, 3-5 points
Mouse 10-15/point, 3-5 points
Tail vein Rat 250 (200g body weight)
Mouse 100
Liver AAV8 or AAV9 Tail vein Rat 200 (200g body weight)
Mouse 100
Whole brain AAV-PHP.eB AAV-PHP.B Tail vein Rat 200 (200g body weight)
Mouse 100
Lateral ventricle AAV9 Stereotactic Rat 1-5
Mouse 1-5
Brain tissue AAV9 Stereotactic Rat 2-3
Mouse 1-2
Fat AAV9 Intraperitoneal injection for intraperitoneal fat Rat 300
Mouse 150-200
in situ injection for subcutaneous fat Rat 10-15/point, 3-5 points
Mouse 10-15/point, 3-5 points
Skeletal muscle AAV1 or AAV9 In situ injection Rat 10-15/point, 3-5 points
Mouse 10-15/point, 3-5 points
Eyes AAV2, AAV10 or AAV-DJ vitreous chamber injection Rat 3-5
Mouse 1-3
subretinal space injection Rat 1-2
Mouse 1-2
Lung AAV6 Intratracheal injection Rat 100 (200g weight)
Mouse 50-75
Kidney AAV2 or AAV9 Renal pelvis injection Rat 10-15/point, 3-5 points
Mouse 10-15/point, 3-5 points
Intestine AAV1 or AAV5 Enema Rat 200 (200g weight)
Mouse 100

Detailed information of AAV in vivo animal injection protocol can be seen in AAV User Manual.



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