SOLIDEX™ CD14+ Cell Isolation: High-Purity Monocyte Separation Kits

SOLIDEX™ CD14+ Cell Isolation Solution: High-Purity Monocyte Separation

1. Introduction to High-Purity CD14+ Monocyte Isolation

CD14+ cells, predominantly monocytes, are critical components of the innate immune system, typically constituting **5-10% of peripheral blood mononuclear cells (PBMCs)**. These versatile cells are essential for initiating immune responses, differentiating into macrophages or dendritic cells (DCs), and regulating inflammation through cytokine secretion (e.g., TNF-$$\alpha$$ and IL-6).

The SOLIDEX™ CD14+ Cell Isolation Solution utilizes advanced magnetic bead technology to ensure the isolation of highly viable and functional monocytes. Our proprietary technology offers researchers and clinicians a robust, scalable method for separating CD14+ cells, whether for basic immunology research or for developing **GMP-grade cell therapies**.

We provide comprehensive solutions for both **Positive Selection** (direct enrichment of CD14+ cells) and **Depletion** (removal of CD14+ cells), catering to diverse experimental and clinical requirements.

2. SOLIDEX™ Isolation Methodologies

Applications for Positive Selection of CD14+ Monocytes

Research: Ideal for generating macrophages ex vivo, cytotoxicity studies, and migration assays to assess monocyte/macrophage function in various inflammatory models, including phagocytic activity and chemotactic responses.

Clinical & Translational: Positive selection is crucial for preparing clinical-grade DC vaccines (where autologous DCs are differentiated from monocytes) and for maintenance therapies in oncology. This method enables the high-throughput isolation necessary for cell manufacturing and transplantation protocols.

Positive Selection Products

Cat. No	Product Name	Details
GM-Tg-hg-T23212-iso-nanoIMB	SOLIDEX™ Human CD14 Isolation Nanobeads (Column-based)	View Details
GM-Tg-hg-T23212-iso-nanoIMB-CF	SOLIDEX™ Human CD14 Isolation Nanobeads (Column-free)	View Details
GM-Tg-hg-T23212-iso-rel-kit	SOLIDEX™ Indirect Releasable Human CD14+ Cell Isolation Kit (Column-based)	View Details
GM-Tg-hg-T23212-iso-rel-kit-CF	SOLIDEX™ Indirect Releasable Human CD14+ Cell Isolation Kit (Column-free)	View Details
GM-Tg-hg-T23212-iso-kit	SOLIDEX™ Indirect Human CD14+ Cell Isolation Kit (Column-based)	View Details
GM-Tg-hg-T23212-iso-kit-CF	SOLIDEX™ Indirect Human CD14+ Cell Isolation Kit (Column-free)	View Details

Applications for CD14+ Cell Depletion

Research: Used to study the function of non-myeloid immune cells by eliminating the regulatory or suppressive effects of CD14+ monocytes/macrophages. This facilitates the analysis of T cell or NK cell responses in isolation.

Clinical & Translational: CD14 depletion is a crucial step in preparing T cells for CAR-T manufacturing. Removing myeloid cells minimizes contamination, thereby enhancing CAR-T cell transduction and expansion success, leading to higher purity and improved therapeutic activity.

Depletion Products

Cat. No	Product Name	Details
GM-Tg-hg-T23212-dep-nanoIMB	SOLIDEX™ Human CD14+ Cell Depletion Nanobeads (Column-based)	View Details
GM-Tg-hg-T23212-dep-kit	SOLIDEX™ Indirect Human CD14+ Cell Depletion Kit (Column-based)	View Details

3. Key Features of SOLIDEX™ CD14+ Isolation

> 95%

High Purity Guarantee

Achieve consistently high purity for downstream applications like flow cytometry, cell culture, and functional assays.

Scalable

Clinical & Large-Scale Ready

Kits are designed for scalability, supporting both small research needs and large-volume GMP manufacturing protocols.

Flexible

Column-Based & Column-Free Options

Choose the format that fits your laboratory setup: traditional column separation or the simplified column-free method.

4. Validation Data & Performance Guarantee

All SOLIDEX™ CD14+ Isolation Kits undergo rigorous quality control to ensure superior purity, yield, and viability. Performance is validated across multiple donors and flow cytometry systems.

Typical Isolation Purity (Flow Cytometry Data)

The data below shows representative flow cytometry results demonstrating the high purity of isolated CD14+ cells compared to the starting PBMC population.

[Placeholder for Flow Cytometry Graph: Purity & Yield]

Figure 1. Flow Cytometry Analysis of PBMC (Pre-Sort) vs. Isolated CD14+ Cells (Post-Sort).

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