Human ATP6V0E1/ATP6H/ATP6V0E ORF/cDNA clone-Lentivirus particle (NM_003945)

Cat. No.: vGMLP002656

Pre-made Human ATP6V0E1/ATP6H/ATP6V0E Lentiviral expression plasmid for ATP6V0E1 lentivirus packaging, ATP6V0E1 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

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Product information

Catalog No. Product Name lentivirus Grade lentivirus quantity
vGMLP002656 Human ATP6V0E1 Lentivirus particle Pilot Grade 1.0E+8TU
5.0E+8TU
1.0E+9TU
Research Grade 1.0E+8TU
5.0E+8TU
1.0E+9TU
GMP-like Grade inquiry
GMP Grade inquiry


Product Description

Catalog ID vGMLP002656
Gene Name ATP6V0E1
Accession Number NM_003945
Gene ID 8992
Species Human
Product Type Lentivirus particle (overexpression)
Insert Length 246 bp
Gene Alias ATP6H,ATP6V0E,M9.2,Vma21,Vma21p
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGCGTATCACGGCCTCACTGTGCCTCTCATTGTGATGAGCGTGTTCTGGGGCTTCGTCGGCTTCTTGGTGCCTTGGTTCATCCCTAAGGGTCCTAACCGGGGAGTTATCATTACCATGTTGGTGACCTGTTCAGTTTGCTGCTATCTCTTTTGGCTGATTGCAATTCTGGCCCAACTCAACCCTCTCTTTGGACCGCAATTGAAAAATGAAACCATCTGGTATCTGAAGTATCATTGGCCTTGA
ORF Protein Sequence MAYHGLTVPLIVMSVFWGFVGFLVPWFIPKGPNRGVIITMLVTCSVCCYLFWLIAILAQLNPLFGPQLKNETIWYLKYHWP

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-IP0392-Ab Anti-ATP6V0E1 monoclonal antibody
    Target Antigen GM-Tg-g-IP0392-Ag ATP6V0E1 protein
    ORF Viral Vector pGMLP002656 Human ATP6V0E1 Lentivirus plasmid
    ORF Viral Vector vGMLP002656 Human ATP6V0E1 Lentivirus particle


    Target information

    Target ID GM-IP0392
    Target Name ATP6V0E1
    Gene ID 8992, 11974, 703342, 94170, 101091676, 403729, 338075, 100069785
    Gene Symbol and Synonyms ATP6H,Atp6k,ATP6V0E,ATP6V0E1,dsr-1,Dsr-1A,M9.2,Vma21,Vma21p
    Uniprot Accession O15342
    Uniprot Entry Name VA0E1_HUMAN
    Protein Sub-location Introcelluar Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000113732
    Target Classification Not Available

    This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008]



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