Human MID1/BBBG1/FXY ORF/cDNA clone-Lentivirus particle (NM_000381)

Cat. No.: vGMLP002950

Pre-made Human MID1/BBBG1/FXY Lentiviral expression plasmid for MID1 lentivirus packaging, MID1 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

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(antibodies, antigen, VLP, mRNA, ORF viral vector, etc)

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Product information

Catalog No. Product Name lentivirus Grade lentivirus quantity
vGMLP002950 Human MID1 Lentivirus particle Pilot Grade 1.0E+8TU
5.0E+8TU
1.0E+9TU
Research Grade 1.0E+8TU
5.0E+8TU
1.0E+9TU
GMP-like Grade inquiry
GMP Grade inquiry


Product Description

Catalog ID vGMLP002950
Gene Name MID1
Accession Number NM_000381
Gene ID 4281
Species Human
Product Type Lentivirus particle (overexpression)
Insert Length 2004 bp
Gene Alias BBBG1,FXY,GBBB1,MIDIN,OGS1,OS,OSX,RNF59,TRIM18,XPRF,ZNFXY
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTCCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCCTACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGCCAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTGTGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCAGCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGAACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACGTCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATTGGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAACAGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCGTTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATTCCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAGAATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGACACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATATTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCAAGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAACCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCCAAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCAAGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGATAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCCATTGGTCTTGCTTACAAATCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCCTGGGCGCTCTGCCGCTGCAACAATAACTGGGTGGTGAGACACAATAGCAAGGAAATCCCCATTGAGCCTGCCCCCCACCTCCGGCGCGTGGGCATCCTGCTGGACTATGATAACGGCTCTATCGCCTTTTATGATGCTTTGAACTCCATCCACCTCTACACCTTCGACGTCGCATTTGCGCAGCCTGTTTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGCTCCCTATCCCAGACCATTTGGACTGCACAGAGCAGCTGCCGTGA
ORF Protein Sequence METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-IP1190-Ab Anti-MID1 monoclonal antibody
    Target Antigen GM-Tg-g-IP1190-Ag MID1 protein
    ORF Viral Vector pGMLP002950 Human MID1 Lentivirus plasmid
    ORF Viral Vector vGMLP002950 Human MID1 Lentivirus particle


    Target information

    Target ID GM-IP1190
    Target Name MID1
    Gene ID 4281, 17318, 709683, 54252, 101085021, 491737, 534604, 100049999
    Gene Symbol and Synonyms 61B3-R,BBBG1,DXHXS1141,FXY,GBBB,GBBB1,MID1,MIDIN,Midline1,OGS1,OS,OSX,RNF59,TRIM18,XPRF,ZNFXY
    Uniprot Accession O15344
    Uniprot Entry Name TRI18_HUMAN
    Protein Sub-location Introcelluar Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000101871
    Target Classification Not Available

    The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]



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