2019 nCoV (SARS2 coronavirus) ORF3a pcDNA3.1(+) vector

Pre-made gene ORF plasmids for 2019 nCoV (SARS CoV-2 coronavirus, COVID-19)
Spike protein(S protein):Spike full length, Spike RBD,Spike mutation(D614G mutataion,
S943P,V367F, G476S, V483A, H49Y, Q239K, A831V, P1263L, D839Y/N/E,D839Y, D839N, D839E

Cat No.	2019 nCoV related Gene	Gene &Vector description of 2019 nCoV	Vector	Reporter	Tag	Coden Optimized	Order
GMV-V-2019nCoV-051	Spike(S1+S2)	pGMLV-2019nCoV-spike (S1+S2,C-6His)	Lentiviral vector	Zsgreen	C-6His	coden optimized for mamamlian
GMV-V-2019nCoV-001	Spike(S1+S2)	pGMLV-2019nCoV-spike(S1+S2,C-6His)	Lentiviral vector	Zsgreen	C-6His	No
GMV-V-2019nCoV-017	Spike(S1+S2)	pGM-2019nCoV-spike protein (S protein,S1+S2)	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-008	Spike(S1+S2)	pGM-2019nCoV-spike protein (S protein,S1+S2)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-053	Spike(S1+S2)	Ad-2019nCoV-Spike(S1+S2,C-3FLAG)	Pre-made adenovirus	null	C-3FLAG	coden optimized for mamamlian
GMV-V-2019nCoV-047	Spike(S1+S2)	Ad-2019nCoV-Spike(S1+S2,C-3FLAG)	Pre-made adenovirus	EGFP	C-3FLAG	No
GMV-V-2019nCoV-052	Spike(S1+S2)	pGMAD-2019nCoV-spike (S protein,S1+S2,C-3FLAG)	Adenoviral vector	null	C-3FLAG	coden optimized for mamamlian
GMV-V-2019nCoV-004	Spike(S1+S2)	pGMAD-2019nCoV-spike (S protein,S1+S2,C-3FLAG)	Adenoviral vector	EGFP	C-3FLAG	No
GMV-V-2019nCoV-035	Spike(S1+S2)	pGM-2019nCoV-spike protein (S protein, S1+S2)	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-037	Spike(S1+S2)	pGM-2019nCoV-spike protein (S protein, S1+S2)	pET-28a(+)	null	His	No
GMV-V-2019nCoV-002	Spike(S1)	pGMLV-2019nCoV-S1(C-6His)	Lentiviral vector	Zsgreen	C-6His	No
GMV-V-2019nCoV-005	Spike(S1)	pGMAD-2019nCoV-S1(C-3FLAG)	Adenoviral vector	EGFP	C-3FLAG	No
GMV-V-2019nCoV-048	Spike(S1)	Ad-2019nCoV-Spike(S1 protein, C-3FLAG)	Pre-made adenovirus	EGFP	C-3FLAG	No
GMV-V-2019nCoV-003	Spike RBD	pGMLV-2019nCoV-Spike RBD(C-6His)	Lentiviral vector	Zsgreen	C-6His	No
GMV-V-2019nCoV-007	Spike RBD	pGMAAV-2019nCoV-Spike RBD(C-3FLAG)	AAV vector	Zsgreen	C-3FLAG	No
GMV-V-2019nCoV-050	Spike RBD	AAV-2019nCoV-Spike (S protein RBD, C-3FLAG)	Pre-made AAV	Zsgreen	C-3FLAG	No
GMV-V-2019nCoV-006	Spike RBD	pGMAD-2019nCoV-Spike RBD(C-3FLAG)	Adenoviral vector	EGFP	C-3FLAG	No
GMV-V-2019nCoV-049	Spike RBD	Ad-2019nCoV-Spike(S protein RBD, C-3FLAG)	Pre-made adenovirus	EGFP	C-3FLAG	No
GMV-V-2019nCoV-054	Spike(S1+S2) D614G mutation	pGM-2019nCoV-spike D614G protein (S protein,S1+S2,D614G)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-055	Spike(S1+S2) D614G mutation	pGMAD-2019nCoV-spike D614G (S protein,S1+S2,D614G)	Adenoviral vector	null	C-3FLAG	coden optimized for mamamlian
GMV-V-2019nCoV-056	Spike(S1+S2) D614G mutation	Ad-2019nCoV-Spike D614G (S protein,S1+S2,D614G)	Pre-made adenovirus	null	C-3FLAG	coden optimized for mamamlian
GMV-V-2019nCoV-057	Spike(S1+S2) S943P mutation	pGM-2019nCoV-spike S943P protein (S protein,S1+S2,S943P)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-058	Spike(S1+S2) V367F mutation	pGM-2019nCoV-spike V367F protein (S protein,S1+S2,V367F)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-059	Spike(S1+S2) G476S mutation	pGM-2019nCoV-spike G476S protein (S protein,S1+S2,G476S)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-060	Spike(S1+S2) V483A	pGM-2019nCoV-spike V483A protein (S protein,S1+S2,V483A)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-061	Spike(S1+S2)H49Y mutation	pGM-2019nCoV-spike H49Y protein (S protein,S1+S2,H49Y)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-062	Spike(S1+S2) Q239K mutation	pGM-2019nCoV-spike Q239K protein (S protein,S1+S2,Q239K)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-063	Spike(S1+S2) A831V mutation	pGM-2019nCoV-spike A831V protein (S protein,S1+S2,A831V)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-064	Spike(S1+S2) P1263L mutation	pGM-2019nCoV-spike P1263L protein (S protein,S1+S2,P1263L)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-065	Spike(S1+S2) D839Y/N/E-D839Y mutation	pGM-2019nCoV-spike D839Y/N/E-D839Y protein (S protein,S1+S2,D839Y/N/E-D839Y)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-066	Spike(S1+S2) D839Y/N/E-D839N mutation	pGM-2019nCoV-spike D839Y/N/E-D839N  protein (S protein,S1+S2,D839Y/N/E-D839N )	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-067	Spike(S1+S2) D839Y/N/E-D839E mutation	pGM-2019nCoV-spike D839Y/N/E-D839E protein (S protein,S1+S2,D839Y/N/E-D839E)	pcDNA3.1(+)	null	No tag	coden optimized for mamamlian

2019 nCoV (SARS2 coronavirus) gene and vectors

Cat No.	2019 nCoV related Gene	Gene &Vector description of 2019 nCoV	Vector	Reporter	Tag	Coden Optimized	Order
GMV-V-2019nCoV-015	ORF8	pGM-2019nCoV-ORF8	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-024	ORF8	pGM-H2019nCoV-ORF8	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-033	ORF8	pGM-E2019nCoV-ORF8	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-014	ORF7	pGM-2019nCoV-ORF7a	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-023	ORF7	pGM-H2019nCoV-ORF7a	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-032	ORF7	pGM-E2019nCoV-ORF7a	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-013	ORF6	pGM-2019nCoV-ORF6	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-022	ORF6	pGM-H2019nCoV-ORF6	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-031	ORF6	pGM-E2019nCoV-ORF6	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-012	ORF3a	pGM-2019nCoV-ORF3a	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-021	ORF3a	pGM-H2019nCoV-ORF3a	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-030	ORF3a	pGM-E2019nCoV-ORF3a	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-016	ORF10	pGM-2019nCoV-ORF10	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-025	ORF10	pGM-H2019nCoV-ORF10	pCDNA3.1(+)	null	No tag	coden optimized or mamamlian
GMV-V-2019nCoV-034	ORF10	pGM-E2019nCoV-ORF10	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-011	N protein	pGM-2019nCoV-Nucleocapsid Protein (N protein)	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-020	N protein	pGM-2019nCoV-Nucleocapsid Protein (N protein)	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-029	N protein	pGM-2019nCoV-Nucleocapsid Protein (N protein)	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-010	M protein	pGM-2019nCoV-Membrane protein (M protein)	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-019	M protein	pGM-2019nCoV-Membrane protein (M protein)	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-028	M protein	pGM-2019nCoV-Membrane protein (M protein)	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-009	E protein	pGM-2019nCoV-Envelope Protein (E protein)	pcDNA3.1(+)	null	No tag	No
GMV-V-2019nCoV-018	E protein	pGM-2019nCoV-Envelope Protein (E protein)	pCDNA3.1(+)	null	No tag	coden optimized for mamamlian
GMV-V-2019nCoV-027	E protein	pGM-2019nCoV-Envelope Protein (E protein)	pET21a	null	C-His	coden optimized for E.coli
GMV-V-2019nCoV-045	TMPRSS2	pGMLv-hTMPRSS2(C-3FLAG)	Lentiviral vector	Zsgreen	C-3FLAG	No
GMV-V-2019nCoV-041	ACE2	pGMLV-hACE2(C-3FLAG)	Lentiviral vector	Zsgreen	C-3FLAG	No
GMV-V-2019nCoV-042	ACE2	pAD-hACE2(C-3FLAG)	Pre-made Adenovirus	EGFP	C-3FLAG	No
GMV-V-2019nCoV-046	TMPRSS2	pGMLv-mtmprss2(C-3FLAG)	Lentiviral vector	Zsgreen	C-3FLAG	No
GMV-V-2019nCoV-043	ACE2	pAD-mACE2(C-3FLAG)	Pre-made Adenovirus	EGFP	C-3FLAG	No
GMV-V-2019nCoV-044	ACE2	pGMLV-mACE2(C-3FLAG)	Lentiviral vector	Zsgreen	C-3FLAG	No

About SARS-CoV-2 (2019nCoV, novel Coronavirus) Spike protein, S1 Protein, Spike S1-NTD, Spike S1-CTD, Spike-RBD and Spike trimer protein.

1. SARS-CoV-2 (2019nCoV) Spike protein: SARS-CoV-2, a newly emerged pathogen spreading worldwide. The transmembrane spike (S) glycoprotein of SARS-CoV-2 that forms homotrimers protruding from the viral surface is known to mediate coronavirus entry into host cells. It has been reported that spike protein can bind with high affinity to human ACE2 and uses it as an entry receptor to invade target cells.

2. SARS-CoV-2 (2019nCoV) spike S1 protein: The spike protein is a large type I transmembrane glycoprotein comprises two functional subunits, S1 and S2. S1 subunit of spike protein is responsible for binding to the host cell receptor. S2 subunit is responsible for fusion of the viral and cellular membranes.

3. SARS-CoV-2 (2019nCoV) spike S1-NTD and S1-CTD: For most coronaviruses, the N-terminal domain (NTD) of the S1 subunit attaches to cellular carbohydrates and the C-terminal domain of S1 (S1-CTD) binds to a cellular protein receptor. Carbohydrate binding by the S1 N-terminal domain is thought to keep the virus in close proximity to the host cell surface, whereas engagement of specific protein receptors by the S1-CTD is thought to initiate a series of conformational changes in the spike that ultimately result in membrane fusion and delivery of the viral genome to the cytosol.

4. SARS-CoV-2 (2019nCoV) Spike RBD: S1 subunit of spike protein contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor.

5. SARS-CoV-2 (2019nCoV) Spike trimer protein: The transmembrane spike (S) glycoprotein of SARS-CoV-2 usually forms homotrimers protruding from the viral surface. S trimers are extensively decorated with N-linked glycans that are important for proper folding and for modulating accessibility to host proteases and neutralizing Abs. It has been found that S glycoprotein trimers in highly pathogenic human coronaviruses appear to exist in partially opened states, while they remain largely closed in human coronaviruses associated with common colds.

About SARS-CoV-2 (2019nCoV, novel Coronavirus) Envelop protein (Coronavirus E protein)

1. SARS-CoV-2 (2019nCoV) E protein: E protein is the smallest major structural proteins. It has a N-terminal ectodomain and a C-terminal endodomain with ion channel activity. During the replication cycle, E protein is abundantly expressed inside the infected cell, but only a small portion is incorporated into the virus envelope. The majority of the protein participates in viral assembly and budding. E protein is important in virus production and maturation. Recombinant CoVs without E have been shown to exhibit significantly reduced viral titres, crippled viral maturation, or yield incompetent progeny.

About SARS-CoV-2 (2019nCoV, novel Coronavirus) membrane protein (Coronavirus M protein)

1. SARS-CoV-2 (2019nCoV) M protein: Coronavirus M protein is believed to define the shape of the viral envelope,which contains three transmembrane domains. It has a small N-terminal glycosylated ectodomain and a much larger C-terminal endodomain that extends 6–8 nm into the viral particle. M protein usualy exists as a dimer, and may adopt two different conformations allowing it to promote membrane curvature as well as bind to the nucleocapsid.

About SARS-CoV-2 (2019nCoV, novel Coronavirus) Nucleocapsid protein (Coronavirus N protein)

1. SARS-CoV-2 (2019nCoV) N protein: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

About SARS-CoV-2 (2019nCoV, novel Coronavirus)Non-structure protein (Nsp1-Nsp16)

2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-19¹.

1. Nsp3: Nsp3 (200 kDa) is the largest protein encoded by the coronavirus (CoV) genome. Nsp3 is an essential component of the replication and transcription complex. It comprises various domains, the organization of which differs between CoV genera, due to duplication or absence of some domains. However, the N-terminal region of the Nsp3 is highly conserved among CoV, containing a ubiquitin-like (Ubl) globular fold followed by a flexible, extended acidic-domain (AC domain) rich in glutamic acid (38%). Next to the AC domain is a catalytically active ADP-ribose-1"-phosphatase (ADRP, app-1"-pase) domain (also called macro domain or X domain) thought to play a role during synthesis of viral subgenomic RNAs. SARS Unique Domain (SUD), a domain not yet identified in other coronaviruses from alphacoronavirus and betacoronavirus, follows next. The SUD domain binds oligonucleotides known to form G-quadruplexes. Downstream of the SUD domain is a second Ubl domain and the catalytically active PLpro domain that proteolytically processes the Nsp1/2, Nsp2/3 and Nsp3/4 cleavage sites. Downstream of PLpro are found a nucleic acid-binding domain (NAB) with a nucleic acid chaperon function, which is conserved in betacoronavirus and gammacoronavirus, and one uncharacterized domain termed the marker domain (G2M). Following the G2M are two predicted double-pass transmembrane domains (TM1–2 and TM3–4), a putative metal binding region (ZN) and the Y domain of unknown function (subdomains Y1–3).

2. Nsp5: Nsp5 protease (3CLpro; Mpro) mediates processing at 11 distinct cleavage sites, including its own autoproteolysis, and is essential for virus replication. Nsp5 exhibits a conserved three-domain structure and catalytic residues.

3. Nsp10: Nsp10 (18 kDa) is well conserved among coronaviruses and encoded by ORF1a. It's thought to serve as an important multifunctional cofactor in replication. Nsp10 was shown to interact with itself, as well as with Nsp1, Nsp7, Nsp14, and Nsp16. The important role of Nsp10 is responsible for RNA synthesis. It was shown that a murine hepatitis virus (MHV) temperature-sensitive mutant carrying a non-synonymous mutation in the Nsp10 coding sequence had a defect in minus-strand RNA synthesis at non-permissive temperatures.

4. Nsp12: Nsp12 (102 kDa) is a multidomain RNA polymerase, which is the most conserved protein in coronaviruses. Nsp12 contains an RNA-dependent RNA polymerase (RdRp) domain in its C-terminal, which is essential for the viral replication and transcription.

5. Nsp16: Nsp16 is an SAM-dependent nucleoside-2’O-methyl-transferase (2’O-MTase). The mRNA cap for coronaviruses is completed by Nsp16, which ensures formation of a protective cap-1 structure that prevent recognition by either MDA5 or IFIT proteins. Finally, the NSP16/NSP10 complex finishes coronavirus capping process permitting viral infection with reduced host recognition.

About COVID-19 pandemic, Coronavirus (Coronavirus) and genome of SARS-CoV-2 (2019nCoV)

COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2, a novel coronavirus) infection.The 2019-nCoV genome was annotated to possess 14 ORFs encoding 27 proteins1.

Collection of COVID-19 landscape knowledge base

Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine - A landscape for vaccine technology against infectious disease, COVID-19 and tumor.

COVID-19 landscape Knowledge Base

An Insight of comparison between COVID-19 (2019-nCoV disease) and SARS in pathology and pathogenesis

Landscape Coronavirus Disease 2019 test (COVID-19 test) in vitro -- A comparison of PCR vs Immunoassay vs Crispr-Based test

References