Cowbone Ridge virus antibody and antigen (recombinant protein)

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Diagnostic anti-Cowbone Ridge virus antibodies pairs and antigen for animal health (animal Dog/Canine infectious disease) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P009-Ag01	Recombinant Cowbone Ridge virus protein	$3090.00
GMP-VT-P009-Ab01	Anti-Cowbone Ridge virus mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P009-Ab02	Anti-Cowbone Ridge virus mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P009-Ag01
Product Name	Recombinant Cowbone Ridge virus protein
Pathogen	Cowbone Ridge virus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Cowbone Ridge virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Cowbone Ridge virus level test of animal Dog/Canine infectious disease.
Tag	His
Product description	Recombinant Cowbone Ridge virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P009-Ab01,GMP-VT-P009-Ab02
Pathogen	Cowbone Ridge virus
Product Name	Anti-Cowbone Ridge virus mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Cowbone Ridge virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Cowbone Ridge virus antibodies in Cowbone Ridge virus level test of animal Dog/Canine infectious disease.
Product description	Anti-Cowbone Ridge virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Cowbone Ridge virus antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Pathogen

Cowbone Ridge virus (CRV) is a pathogenic virus that causes Rift Valley fever in ruminant livestock, particularly cattle, sheep, and goats. The virus was first isolated in 1976 from the blood of a cow at Cowbone Ridge Farm in Mississippi, USA. Since then, CRV has been reported in several African countries, including Kenya, Sudan, South Africa, and Madagascar. The virus is transmitted to animals through the bites of infected mosquitoes, primarily Aedes and Culex species.

CRV is an enveloped single-stranded RNA virus with a negative-sense genome that belongs to the family Phenuiviridae. It is classified within the genus Phlebovirus, which comprises other arthropod-borne viruses such as sandfly fever viruses and severe fever with thrombocytopenia syndrome virus (SFTSV). CRV is further divided into eight different genetic lineages based on phylogenetic analysis of viral sequences. These lineages are distributed across different geographic regions and host species, suggesting that the virus has undergone multiple divergent evolution events.

The genome of CRV consists of three segments designated as L, M, and S, which encode different viral proteins. The L segment encodes the RNA-dependent RNA polymerase (RdRp), which is responsible for genome replication and transcription. The M segment encodes the envelope glycoproteins Gn and Gc, which are involved in virus entry, fusion, and budding. The S segment encodes the nucleoprotein (N), which binds to viral RNA and forms ribonucleoprotein complexes with RdRp and other proteins.

The replication cycle of CRV begins with the attachment of virions to specific cell surface receptors, followed by endocytosis and uncoating of the virus particle. The viral RNA genome is then released into the cytoplasm, where it serves as a template for RdRp to produce copies of viral mRNA and genome RNA. The newly synthesized viral proteins are transported to the endoplasmic reticulum, where they undergo post-translational modifications and assemble with viral RNA to form functional ribonucleoprotein particles. The mature virions are then released from infected cells through budding or lysis.

The infection of ruminants with CRV can result in a range of clinical outcomes. Many animals remain asymptomatic, while others develop a mild to severe form of Rift Valley fever. The symptoms of Rift Valley fever include fever, anorexia, lethargy, muscle and joint pain, and sometimes respiratory distress and bleeding disorders. In severe cases, the disease can progress to encephalitis, retinitis, and liver failure, leading to death. The severity of the disease depends on several factors, including the virulence of the virus strain, the age and immune status of the animal, and environmental factors such as temperature and humidity.

The diagnosis of CRV infection relies on several laboratory methods, including serological assays, nucleic acid detection, and virus isolation. Serological tests are used to detect antibodies against CRV in serum samples collected from animals, which can confirm past exposure or active infection. ELISAs and neutralization assays are commonly used for this purpose. Nucleic acid amplification tests such as PCR and RT-PCR can detect the presence of viral RNA in various types of tissue samples, blood, and other body fluids. These tests target different segments of the viral genome, such as the L, M, or S gene, and allow for rapid and specific detection of CRV. Virus isolation from clinical samples can also be attempted using cell culture or suckling mice. However, virus isolation is a time-consuming process that requires specialized facilities and expertise.

In conclusion, Cowbone Ridge virus is a significant pathogen of ruminant livestock that can cause severe disease and economic losses. The virus has a complex genomic organization and replication cycle, which require careful investigation to develop effective diagnostic tools and vaccines. Ongoing surveillance of CRV circulation in different regions and host populations is essential to prevent and control outbreaks of Rift Valley fever and reduce the impact of this zoonotic disease on animal and human health.

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