Actinobacillus pleuropneumoniae serotype 4 antibody and antigen (recombinant protein)

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Diagnostic anti-Actinobacillus pleuropneumoniae serotype 4 antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease swine pleuropneumonia) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P128-Ag01	Recombinant Actinobacillus pleuropneumoniae serotype 4 protein	$3090.00
GMP-VT-P128-Ab01	Anti-Actinobacillus pleuropneumoniae serotype 4 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P128-Ab02	Anti-Actinobacillus pleuropneumoniae serotype 4 mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P128-Ag01
Product Name	Recombinant Actinobacillus pleuropneumoniae serotype 4 protein
Pathogen	Actinobacillus pleuropneumoniae serotype 4
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Actinobacillus pleuropneumoniae serotype 4 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Actinobacillus pleuropneumoniae serotype 4 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Tag	His
Product description	Recombinant Actinobacillus pleuropneumoniae serotype 4 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P128-Ab01,GMP-VT-P128-Ab02
Pathogen	Actinobacillus pleuropneumoniae serotype 4
Product Name	Anti-Actinobacillus pleuropneumoniae serotype 4 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Actinobacillus pleuropneumoniae serotype 4 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 4 antibodies in Actinobacillus pleuropneumoniae serotype 4 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Product description	Anti-Actinobacillus pleuropneumoniae serotype 4 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 4 antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Actinobacillus pleuropneumoniae (APP) serotype 4 is a pathogenic bacterium that infects pigs, leading to porcine pleuropneumonia, which is a highly contagious respiratory disease of swine. First identified in 1957, APP is a Gram-negative, non-motile, and facultative anaerobic bacterium. It is widespread globally, causing significant economic losses in the pig industry.

The APP genome consists of two chromosomes, with a total size of approximately 2.3 Mb. The genome encodes several proteins, some of which have been shown to be involved in virulence. One such protein is the RTX toxin, which plays a crucial role in the pathogenesis of APP infections. Additionally, several virulence factors are encoded by genes located on plasmids, including the cytolysin apxIA, apxIIA, and apxIIIA, enzymes, and capsule biosynthesis genes.

APP is divided into 18 serotypes based on antigenic differences in the polysaccharide capsule. Serotypes 1 to 5 are considered the most prevalent and virulent types worldwide, with serotype 4 being one of the most frequently isolated in many countries.

The most common route of APP transmission is through respiratory droplets from infected pigs or contaminated environments. Once the bacteria reach the lungs, they quickly colonize and cause severe damage, leading to symptoms such as coughing, fever, dyspnea, anorexia, and depression. In addition to the respiratory tract, APP can also affect other organs, such as the heart, liver, and kidneys.

There are several diagnostic methods available for detecting APP infections, and these include bacterial culture, serological assays, PCR, and ELISA. Bacterial culture involves isolating and growing the pathogen on specific media, while serological assays detect the presence of antibodies against APP in the blood. PCR detects the presence of specific genes, such as apx and rtxA, while ELISA detects the presence of specific proteins, such as the RTX toxin.

Preventing APP infections in pigs involves good management practices that minimize exposure to contaminated environments or sick animals. Vaccines are also available, and they typically target one or more of the virulence factors involved in APP pathogenesis, such as the cytolysin apxIA or the outer membrane protein OMP-P5. Antibiotics may also be used to treat infected animals, although the emergence of antibiotic-resistant strains of APP is a growing concern.

In conclusion, APP serotype 4 is a pathogenic bacterium that poses a significant threat to the pig industry worldwide. The understanding of its genetics, virulence factors, mode of transmission, and diagnostic methods is essential for the prevention and control of the disease.

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