Salmonella enterica Pullorum IpaJ antibody and antigen (recombinant protein)

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Diagnostic anti-Salmonella enterica Pullorum IpaJ antibodies pairs and antigen for animal health (animal Chicken, Turkey infectious disease Pullorum) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P219-Tg001-Ag01	Recombinant Salmonella enterica Pullorum IpaJ protein	$3090.00
GMP-VT-P219-Tg001-Ab01	Anti-Salmonella enterica Pullorum IpaJ mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P219-Tg001-Ab02	Anti-Salmonella enterica Pullorum IpaJ mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P219-Tg001-Ab03	Anti-Salmonella enterica Pullorum IpaJ human monoclonal antibody (mAb)	$3090.00
GMP-VT-P219-Tg001-Ab04	Anti-Salmonella enterica Pullorum IpaJ human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P219-Tg001-Ag01
Product Name	Recombinant Salmonella enterica Pullorum IpaJ protein
Pathogen	Salmonella enterica Pullorum
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Salmonella enterica Pullorum IpaJ antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Salmonella enterica Pullorum level test of animal Chicken, Turkey infectious disease with Pullorum.
Tag	His
Product description	Recombinant Salmonella enterica Pullorum IpaJ proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P219-Tg001-Ab01,GMP-VT-P219-Tg001-Ab02
Pathogen	Salmonella enterica Pullorum
Product Name	Anti-Salmonella enterica Pullorum IpaJ mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Salmonella enterica Pullorum IpaJ antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Salmonella enterica Pullorum antibodies in Salmonella enterica Pullorum level test of animal Chicken, Turkey infectious disease with Pullorum.
Product description	Anti-Salmonella enterica Pullorum IpaJ mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Salmonella enterica Pullorum antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P219-Tg001-Ab03,GMP-VT-P219-Tg001-Ab04
Pathogen	Salmonella enterica Pullorum
Product Name	Anti-Salmonella enterica Pullorum IpaJ human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Salmonella enterica Pullorum IpaJ antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Salmonella enterica Pullorum antibodies in Salmonella enterica Pullorum level test of animal Chicken, Turkey infectious disease with Pullorum.
Product description	Anti-Salmonella enterica Pullorum IpaJ mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Salmonella enterica serovar Pullorum: Unveiling a Poultry Pathogen

Salmonella enterica serovar Pullorum, commonly referred to as Salmonella Pullorum, is a Gram-negative bacterium that holds a significant place in the field of veterinary medicine and poultry science. This bacterium is a pathogenic serovar within the Salmonella enterica subspecies enterica, known for its distinct ability to induce Pullorum disease in avian hosts, particularly poultry. In this comprehensive overview, we delve into the scientific intricacies of Salmonella Pullorum, from its classification and structural components to the host species it affects, the diseases it causes, and the diagnostic methods employed for its detection.

Classification of Salmonella Pullorum: A Prokaryotic Pathogen

Salmonella Pullorum is unequivocally classified as a prokaryotic bacterium. Within the domain of biology, it falls under the kingdom Bacteria and the phylum Proteobacteria. More specifically, it belongs to the class Gammaproteobacteria. This classification places Salmonella Pullorum among a diverse group of bacteria known for their clinical and ecological importance.

Structural Features: The Genetic and Proteinic Ensemble

The structural features of Salmonella Pullorum unveil a complex genetic and proteinic ensemble that is fundamental to its pathogenicity. The bacterium is equipped with several key components, including:

Flagella: Salmonella species, including Pullorum, possess flagella – whip-like appendages that serve a crucial role in bacterial motility. These flagella allow the bacterium to navigate and establish contact with host cells.

Type III Secretion System (T3SS): A hallmark of pathogenic Salmonella strains is the Type III Secretion System, abbreviated as T3SS. This specialized apparatus enables Salmonella Pullorum to inject virulence factors, often referred to as effector proteins, into host cells. The T3SS is a molecular syringe that is central to the bacterium's ability to manipulate host cellular processes and establish infection.

The interplay of these structural components is a testament to the sophistication of Salmonella Pullorum's adaptation for infecting avian hosts.

Hosts and Diseases: An Avian Affliction

Salmonella Pullorum primarily targets avian species, with chickens and turkeys being its most notable hosts. Within these avian hosts, Salmonella Pullorum can cause a disease known as Pullorum disease. This affliction is characterized by a range of clinical symptoms, including decreased egg production, white diarrhea, and elevated mortality rates, particularly among young poultry. The high degree of contagiousness associated with Pullorum disease makes it a matter of concern within the poultry industry, given its potential to affect flock health and economic stability.

Diagnostic Methods: Unraveling the Presence of Salmonella Pullorum

Accurate and timely diagnosis of Salmonella Pullorum infections is pivotal for the control and prevention of its spread within poultry populations. Several diagnostic methods are employed for this purpose, encompassing both traditional and molecular techniques. These include:

Bacterial Culture: The isolation of Salmonella Pullorum through bacterial culture remains a tried-and-true method. Selective media, such as Selenite broth or Rappaport-Vassiliadis agar, are used to encourage the growth of the bacterium.

Serological Tests: Various serological tests are employed to detect specific antibodies generated by avian hosts in response to Salmonella Pullorum infection. The Rapid Plate Agglutination (RPA) test and the Tube Agglutination Test are examples of such tests.

Polymerase Chain Reaction (PCR): Molecular methods, like PCR, play a pivotal role in detecting the presence of Salmonella Pullorum. PCR assays target specific genetic markers, with the "invA" gene being a commonly used marker in this context.

Enzyme-Linked Immunosorbent Assay (ELISA): ELISA tests are highly efficient in detecting antibodies produced as a result of Salmonella Pullorum infection. They provide a quantitative measure of antibody levels, aiding in the diagnosis.

Mass Spectrometry: Advanced techniques such as mass spectrometry are utilized for pathogen identification by analyzing the protein profiles of the bacterium.

The targeted genes and proteins in these diagnostic methods include:

invA Gene: PCR-based assays often focus on the invA gene, a genetic marker specific to Salmonella species, including Pullorum.

Specific Antibodies: Serological and ELISA tests aim to detect antibodies elicited as part of the host's immune response to Salmonella Pullorum infection.

These diagnostic techniques, with their emphasis on genetic markers and immune responses, play a vital role in the precise identification of Salmonella Pullorum infections within poultry populations. They are an essential tool for veterinarians and poultry producers seeking to mitigate the economic impact of Pullorum disease and ensure the health and well-being of their flocks.

In conclusion, Salmonella enterica serovar Pullorum, though diminutive in size, is a bacterium of considerable significance, particularly within the context of poultry health. Its pathogenicity and the potential for disease transmission in avian hosts underscore the importance of accurate diagnostic methods and a deeper understanding of its structural and genetic intricacies. Ongoing research and vigilant monitoring of this pathogen are vital to safeguarding the poultry industry and public health alike.

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