Salmonella enterica subsp. Typhimurium antibody and antigen (recombinant protein)
Diagnostic anti-Salmonella enterica subsp. Typhimurium antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease gastrointestinal disease) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT
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Product information
Catalog No. | Description | US $ Price (per mg) |
---|---|---|
GMP-VT-P276-Ag01 | Recombinant Salmonella enterica subsp. Typhimurium protein | $3090.00 |
GMP-VT-P276-Ab01 | Anti-Salmonella enterica subsp. Typhimurium mouse monoclonal antibody (mAb) | $3090.00 |
GMP-VT-P276-Ab02 | Anti-Salmonella enterica subsp. Typhimurium mouse monoclonal antibody (mAb) | $3090.00 |
Size: 1mg | 10mg | 100mg
Product Description
Cat No. | GMP-VT-P276-Ag01 |
Product Name | Recombinant Salmonella enterica subsp. Typhimurium protein |
Pathogen | Salmonella enterica subsp. Typhimurium |
Expression platform | E.coli |
Isotypes | Recombinant Antigen |
Bioactivity validation | Anti-Salmonella enterica subsp. Typhimurium antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Salmonella enterica subsp. Typhimurium level test of animal Cat/Feline, Dog/Canine, Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with gastrointestinal disease. |
Tag | His | Product description | Recombinant Salmonella enterica subsp. Typhimurium proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays. |
Formulation | Lyophilized from sterile PBS, PH 7.4 |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Cat No. | GMP-VT-P276-Ab01,GMP-VT-P276-Ab02 |
Pathogen | Salmonella enterica subsp. Typhimurium |
Product Name | Anti-Salmonella enterica subsp. Typhimurium mouse monoclonal antibody (mAb) |
Expression platform | CHO |
Isotypes | Mouse IgG |
Bioactivity validation | Recombinant Salmonella enterica subsp. Typhimurium antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Salmonella enterica subsp. Typhimurium antibodies in Salmonella enterica subsp. Typhimurium level test of animal Cat/Feline, Dog/Canine, Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with gastrointestinal disease. |
Product description | Anti-Salmonella enterica subsp. Typhimurium mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Salmonella enterica subsp. Typhimurium antibodies./td> |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays. |
Formulation | Lyophilized from sterile PBS, PH 7.4 |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Reference
Validation Data
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Pathogen
Introduction:
Salmonella Typhimurium, a gram-negative, facultative anaerobic bacterium, is a formidable pathogen associated with a spectrum of diseases, particularly gastroenteritis in humans. This bacterium belongs to the family Enterobacteriaceae and holds significant clinical and epidemiological importance due to its global prevalence. In this detailed exploration, we will delve into various aspects of this pathogen, from its taxonomy to its diagnostic methods, in order to offer a comprehensive understanding.
Taxonomy and Classification:
Salmonella Typhimurium is classified within the domain Bacteria, phylum Proteobacteria, class Gammaproteobacteria, order Enterobacterales, and family Enterobacteriaceae. This family encompasses a diverse group of gram-negative bacteria, many of which are common inhabitants of the human intestinal tract. Salmonella Typhimurium is one of the many serovars within the Salmonella genus, which also includes Salmonella enterica serovar Enteritidis, Salmonella enterica serovar Typhi, and others. Each serovar is characterized by its specific antigenic properties.
Structure and Molecular Components:
The structure of Salmonella Typhimurium is integral to its pathogenicity. It possesses a gram-negative cell wall composed of an outer membrane, periplasmic space, inner membrane, and cytoplasm. The outer membrane is rich in lipopolysaccharides (LPS), which are known for their endotoxic properties, often inciting an immune response in infected hosts.
Notably, this pathogen carries specific virulence determinants within its pathogenicity islands. Two significant islands are Salmonella pathogenicity island 1 (SPI-1) and Salmonella pathogenicity island 2 (SPI-2). SPI-1 is responsible for the invasion of epithelial cells, a crucial step in establishing infection. It contains genes encoding a type III secretion system and effectors, facilitating the injection of proteins into host cells. SPI-2, on the other hand, is essential for intracellular survival within host cells, enabling Salmonella to evade the host immune response.
One of the key genes in Salmonella Typhimurium is fliC, which encodes flagellin, a protein that forms the flagellar filament. Flagella provide motility to the bacterium, allowing it to swim through viscous environments and facilitating host invasion.
Host Range and Diseases Caused:
Salmonella Typhimurium is known for its broad host range, which includes mammals, birds, reptiles, and humans. It is a common cause of salmonellosis, a foodborne illness in humans. When ingested, this bacterium can lead to various clinical manifestations, with symptoms such as diarrhea, fever, and abdominal cramps being common. While salmonellosis is often self-limiting, in severe cases, the pathogen can disseminate from the intestinal tract, leading to systemic infections. This can result in severe complications and even be life-threatening, particularly in individuals with weakened immune systems.
Diagnostic Methods:
Accurate and timely diagnosis of Salmonella Typhimurium infections is crucial for patient management and outbreak control. Diagnostic methods have evolved over the years, and the choice of method depends on factors such as the patient's condition, the clinical setting, and the required turnaround time.
a. Culture-Based Methods: Traditional culture-based methods involve the isolation of the bacterium from clinical samples. This typically involves the inoculation of the sample onto selective agar media, such as Xylose Lysine Deoxycholate (XLD) agar or Salmonella-Shigella agar. Salmonella Typhimurium colonies exhibit specific characteristics on these media, aiding in their identification.
b. Serological Assays: Serological assays are used to detect specific antibodies produced by the host in response to Salmonella infection. Enzyme-linked immunosorbent assay (ELISA) and agglutination tests are common serological methods. However, these tests may not be as sensitive or specific as molecular methods.
c. Molecular Methods: Molecular techniques, such as Polymerase Chain Reaction (PCR), have gained prominence due to their high sensitivity and specificity. PCR can target specific genes in Salmonella Typhimurium for identification. The invA gene is one such target, as it is characteristic of Salmonella species. Additionally, genes within the virulence-associated pathogenicity islands, notably SPI-1 and SPI-2, can be targeted for strain-specific identification.
Conclusion:
Salmonella Typhimurium, a member of the Enterobacteriaceae family, is a versatile and virulent pathogen. Its wide host range and potential for causing severe illness make it a significant public health concern. A deep understanding of its taxonomy, structure, and diagnostic methods is essential for both clinicians and researchers working to mitigate its impact on human and animal health. Advances in diagnostic techniques continue to improve our ability to detect and manage infections, ultimately contributing to the control and prevention of Salmonella Typhimurium-related diseases.
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