Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression

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Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre)

Cat No. Pre-made Cre Viral vector Protomer Promoter Tissue Specificity Promoter Tissue
Specificity characteristics
GMV-AdCre-01Ad-CMV-CreAdenovirusCMV Total Total
GMV-AdCre-02Ad-CMV-Cre-GFPCMV Total Total
GMV-AAV9Cre-01AAV9-CMV-CreAAV

GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option:
· AAV1-Cre
· AAV2-Cre
· AAV2 variant (Y444F)-Cre
· AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre
· AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre
· AAV2 variant(AAV2.7m8)-Cre
· AAV5-Cre
· AAV6-Cre
· AAV8-Cre
· AAV8-1m-Cre
· AAV8-2m-Cre
· AAV8 variant (Y733F, Y447F, Y275)-Cre
· AAV9-Cre
· AAV-Rh.10-Cre
· AAV-DJ-Cre
· AAV-DJ/8-Cre
· AAV-Retro (Retrograde)-Cre
· AAV-PHP.B-Cre
· AAV9-PHP.A-Cre
· AAV-PHP.eB-Cre
· AAV-PHP.S-Cre
CMV Total Total
GMV-AAV9Cre-02AAV9-CMV-Cre-ZsGreen CMV Total Total
GMV-AAV9Cre-03AAV9-CAG-Cre CAG Total Total
GMV-AAV9Cre-04AAV9-CAG-Cre-ZsGreen CAG Total Total
GMV-AAV9Cre-05AAV9-Syn-Cre-EGFP synapsin (SYN) NeuroNeuron-specific promoter
GMV-AAV9Cre-06AAV9-Syn-Cre synapsin (SYN) NeuroNeuron-specific promoter
GMV-AAV9Cre-07AAV9-CaMKII-Cre-EGFP CaMKIINeuroForebrain glutamate neuron-specific promoter
GMV-AAV9Cre-08AAV9-CaMKII-Cre CaMKIINeuroForebrain glutamate neuron-specific promoter
GMV-AAV9Cre-09AAV9-GFAP-Cre-EGFP GFAPNeuroAstrocyte specific promoter
GMV-AAV9Cre-10AAV9-GFAP-Cre GFAPNeuroAstrocyte specific promoter
GMV-AAV9Cre-11AAV9-cTNT-Cre cTnTheartCardiomyocyte specific promoter
GMV-AAV9Cre-12AAV9-cTNT-Cre-EGFP cTnTheartCardiomyocyte specific promoter
GMV-AAV9Cre-13AAV9-TBG-Cre TBGliverHepatic specific promoter
GMV-AAV9Cre-14AAV9-TBG-Cre-ZsGreen TBGliverHepatic specific promoter
GMV-AAV9Cre-15AAV9-TIE-Cre TIEendothelialEndothelial-specific promoter
GMV-AAV9Cre-16AAV9-cd68-Cre cd68NeuroMicroglia-specific promoter
GMV-AAV9Cre-17AAV9-c-fos-Cre c-fosNeuroExcitatory neuron promoter
GMV-AAV9Cre-18AAV9-mecp2-Cre mecp2NeuroShorter neuron-specific promoter
GMV-AAV9Cre-19AAV9-MHCK7-Cre MHCK7muscleMuscle specific promoter
GMV-AAV9Cre-20AAV9-Rpe65-Cre Rpe65retinaRetinal specific promoter
GMV-AAV9Cre-21AAV9-sm22a-Cre sm22amuscleSmooth muscle specific promoter
GMV-AAV9Cre-22AAV9-Spc5-12-Cre Spc5-12muscleMyocyte specific promoter
GMV-AAV9Cre-23AAV9-Ta1-Cre TalNeuroEarly neuron-specific promoter
GMV-LvCre-01Lv-CMV-CreLentivirusCMV Total Total
GMV-LvCre-02Lv-CMV-Cre-zsgreenCMV Total Total



Abstract

Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI). To date, numerous systems, derived from the basic Cre-loxP system, have been developed to precisely control gene expression at desired time or cells. Here, we briefly introduce Cre-loxP system, and give a summary about its applications, especially viral vectors-mediated Cre expression (such as AAV-Cre or Ad-Cre) for in vitro or in vivo studies.

Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression

To facilitate the use of Cre-DIO system, AAV vectors have been introduced to tissue-specific Cre mouse to mediate tissue-specific gene overexpression. Without injection of AAV vector carrying DOI-GOI system (AAV-DIO-GOI), tissue X-specific Cre mouse cannot express GOI and reporter gene in tissue X. After injection of AAV-DIO-GOI into tissue X, Cre recombinase will interact with lox sites, thus activating the expression of GOI and reporter. Synchronously, the tissue X will be labeled by the reporter gene (Fig. 7). This system utilizes high cell-specific promoter to drive Cre recombinase expression and a strong promoter to drive GOI expression, which ideally occur just at the appropriate time point and specific cells. As a powerful system with high spatiotemporal fidelity, AAV-DIO exhibits great advantages and potential in the study of neural circuits and networks, behavior, specific cell populations (lineage tracing), animal models of disease, as well as in high-throughput ex vivo researches.

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Figure 7. Principle of tissue-specific gene overexpression via AAV-DIO-GOI with tissue-specific Cre mouse. Without AAV-DOI-GOI, the GOI and reporter gene do not express in tissue X. Upon AAV-DOI-GOI administration into the mouse with tissue X-specific Cre, the GOI and reporter gene will be activated in tissue X and the tissue X will be labeled by reporter gene (green). Lox site 1 and lox site 2 are variants of lox sites.


Summary

The Cre-loxP system, especially inducible tissue-specific knockout and viral vector-mediated Cre expression, has been highly utilized in genetics and cell biology research. Nevertheless, it seems that Cre-loxP system will continue to be prevalent in current and future research studies. GeneMedi is proficient in viral vector development and offers kinds of viral vector-based Cre tools, we can provide the best services and products if required.

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