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TrueX®-ONE Multi-Serotype AAV Capsid ELISA Kit (Universal, No Standard)
GeneMedi's TrueX®-ONE Universal AAV Titration Kit (ELISA, No Standard) is a multi-serotype AAV capsid ELISA kit that brings the convenience of multi-serotype detection to AAV vector quantification. Designed to detect and quantify the major AAV serotypes AAV2, AAV5, AAV6, AAV8, and AAV9 using a single ELISA assay format, this universal AAV ELISA supports multi-serotype AAV capsid quantification without the need for serotype-specific reagents.
TrueX® AAV Titration & Quantification Solution
COA
TrueX®-ONE Universal AAV Titration Kit — Order Information
| Product Type | Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|---|
| Universal | GMV-TrueX-ELISA-U-1-96-NS | TrueX®-ONE Universal AAV Titration Kit (ELISA, No Standard) | 1 plate(96 wells) | |
| Standard | GMV-TrueX-ELISA-AAV2-STD | AAV2 Standard, 1.0E+11 capsids/ml | 120ul | $130 |
| Standard | GMV-TrueX-ELISA-AAV5-STD | AAV5 Standard, 1.0E+11 capsids/ml | 120ul | $130 |
| Standard | GMV-TrueX-ELISA-AAV6-STD | AAV6 Standard, 1.0E+11 capsids/ml | 120ul | $130 |
| Standard | GMV-TrueX-ELISA-AAV8-STD | AAV8 Standard, 1.0E+11 capsids/ml | 120ul | $130 |
| Standard | GMV-TrueX-ELISA-AAV9-STD | AAV9 Standard, 1.0E+11 capsids/ml | 120ul | $130 |
TrueX®-ONE Universal AAV ELISA — Specifications
| Catalog number | GMV-TrueX-ELISA-U-1-96-NS |
|---|---|
| Product name | TrueX®-ONE Universal AAV Titration Kit (ELISA, No Standard) |
| Contents | 12 × 8 Determinations |
| Storage | 2–8 °C |
TrueX®-ONE Multi-Serotype AAV ELISA — Kit Components
| Components | Size | Format | Storage |
|---|---|---|---|
| MTP (Microtiter Plate) | 12 × 8-well strips | Vacuum packing with desiccant bag | 2–8 °C |
| Assay Buffer (10x) | 50 mL | Tube, dilute before use | 2–8 °C |
| Biotin conjugated anti-AAV antibody (100x) | 150 μL | Tube, dilute before use | 2–8 °C |
| HRP-SA (HRP labelled streptavidin, 1000x×) | 15 μL | Tube, dilute before use | 2–8 °C |
| TMB | 15 mL | Tube, ready to use | 2–8 °C |
| Stop Solution | 15 mL | Tube, ready to use | 2–8 °C |
TrueX®-ONE Multi-Serotype AAV Capsid Quantification — Validation Data
Figure AAV2, AAV5, AAV6, AAV8, and AAV9 capsid titration curves generated using the GeneMedi ONE Universal AAV Titration Kit (GMV-TrueX-ELISA-U-1-96-NS).
The tables show the standard concentrations of AAV capsids (capsids/ml) and their corresponding optical density (OD450nm) readings. The graphs plot OD450nm values against capsid concentrations, demonstrating a strong correlation between capsid quantity and absorbance across all tested serotypes (AAV2 R2=0.9997, AAV5 R2=0.9994, AAV6 R2=0.9996, AAV8 R2=0.9998, AAV9 R2=0.9997), confirming the assay's sensitivity and accuracy.
TrueX®-ONE Universal AAV Capsid Titer Assay — FAQs
Which serotypes can the TrueX-ONE multi-serotype AAV ELISA quantify?
The TrueX-ONE Universal AAV Titration Kit has capsid titration data for AAV2, AAV5, AAV6, AAV8, and AAV9. These are the five serotypes shown in the product instruction manual; Universal should not be interpreted as a claim that every AAV serotype has been validated.
Does the TrueX-ONE universal AAV titration kit include an AAV standard?
GMV-TrueX-ELISA-U-1-96-NS is the No Standard version and does not include an AAV standard. Absolute AAV capsid titer quantification requires a calibrated reference standard of the same serotype as the test sample. Without a matched homologous serotype standard, the assay can assess relative titer levels across samples, but cannot accurately determine the true absolute titer.
What samples can be tested with the TrueX-ONE AAV ELISA for multiple serotypes?
The TrueX AAV Titration ELISA Kit is designed for quantitative determination of AAV particles in cell culture supernatants and purified virus preparations. The kit is intended for research use only.
How long does the TrueX-ONE universal AAV capsid titer assay take?
The total assay time is approximately 3.5 hours. The sandwich ELISA workflow uses an antibody-coated plate to capture AAV particles, a biotin-conjugated anti-AAV detection antibody, HRP-conjugated streptavidin, and TMB for colorimetric signal generation.
