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With the COVID-19 outbreak in the world, many flights have been cancelled. In order for the customer to receive the goods properly, we use the FedEx Customized Freight (FCF) of Fedex which demands a higher fee. If the delivery fee is more expensive in your area, we will contact you by mail.

Catalot Number Size
(T,Test, formalized in 96 well, one test per well)
Price(In USD) Qty (Quantity) Sum(In USD)
GM-2019nCoV-PSV16-2 100T 3840
GM-2019nCoV-PSV16-2 200T 5440
GM-2019nCoV-PSV16-2 1000T 21760
GM-2019nCoV-PSV16-2 5000T 87040
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Shipping Cost: 760.00
Total:



SARS-CoV-2 spike-N501Y mutation

Background Reading:
Information and products collection of SARS-COV-2 (2019nCOV) UK B.1.1.7 lineage
Information and products collection of SARS-COV-2 (2019nCOV) South Africa 501Y.V2 lineage
Information and products collection of SARS-CoV-2 spike-N501Y mutation

The world is in midst of the COVID-19 pandemic caused by SARS-CoV-2 (2019nCoV) infection. The Spike protein (S-protein) of SARS-CoV-2 (2019nCoV) mediates receptor (ACE2) binding and cell entry and is the dominant target of the immune system. Most mutations and deletions of SARS-CoV-2 occur in the coronavirus spike protein. Spike position N501, one of the key contact residues in the receptor-binding domain (RBD), and experimental data suggest mutation N501Y can increase ACE2 receptor affinity (Starr et al. 2020). N501Y has also been associated with increased infectivity and virulence in a mouse model (Gu et al. 2020).

Recently a novel SARS-COV-2 (2019nCOV) lineage, the B.1.1.7 lineage, with serials of site mutation, shows stronger infection ability in the UK. In SARS-COV-2 B.1.1.7 lineage, most mutations and deletions occur in the coronavirus spike protein. These mutantions also include SARS-CoV-2 spike- mutation. N501Y mutation is also found in the South Africa 501Y.V2 lineage.

GeneMedi pseudotype virus (pseudovirus) of SARS-COV-2 (2019nCOV) Spike-N501Y mutation


GeneMedi codon-optimized spike mammalian expression vector for SARS-COV-2 (2019nCOV) spike-N501Y mutation

Description



The outbreak of COVID-19, caused by SARS-CoV-2 (2019-nCoV), has been a global public health threat and caught the worldwide concern. GeneMedi has developed SARS-CoV-2 wildtype and mutation variants pseudovirus production system, from which the SARS-CoV-2 wildtype and mutation variants pseudotyped virus can be handled in biosafety level 2 (BSL-2).

GeneMedi develop COVID-19 related SARS-CoV-2 (2019-nCoV)Pseudotyped virus of SARS-CoV-2 Spike Mutation Variants including D614G,S943P,V367F, G476S, V483A, H49Y, Q239K, A831V, P1263L,D839Y/N/E:D839Y,D839N,D839E,P2-mutated Spike protein trimer variant (P2-mutant, S1/S2 cleavage site(furin cleavage sequence)-mutant, trimerization modified), Spike(S1+S2) - B.1.1.7 lineage, Spike(S1+S2)-N501Y mutation, Spike(S1+S2)-HV 69-70 Deletion mutation(ΔH69/ΔV70) and so on.

SARS-CoV-2 Pseudotyped virus with Spike D614G mutation:Increasing frequency and global distribution.
SARS-CoV-2 Pseudotyped virus with Spike S943P mutation:It is found only in Belgium
SARS-CoV-2 Pseudotyped virus with Spike V367F mutation:
SARS-CoV-2 Pseudotyped virus with Spike G476S mutation
SARS-CoV-2 Pseudotyped virus with Spike V483A mutation
SARS-CoV-2 Pseudotyped virus with Spike H49Y mutation
SARS-CoV-2 Pseudotyped virus with Spike Q239K mutation
SARS-CoV-2 Pseudotyped virus with Spike A831V mutation
SARS-CoV-2 Pseudotyped virus with Spike P1263L mutation
SARS-CoV-2 Pseudotyped virus with Spike D839Y/N/E-D839Y mutation
SARS-CoV-2 Pseudotyped virus with Spike D839Y/N/E-D839N mutation
SARS-CoV-2 Pseudotyped virus with Spike D839Y/N/E-D839E mutation
SARS-CoV-2 Pseudotyped virus with P2-mutated Spike protein trimer variant (P2-mutant, S1/S2 cleavage site (furin cleavage sequence)-mutant,trimerization modified) mutation
SARS-CoV-2 Pseudotyped virus with Spike of SARS-COV-2 B.1.1.7 lineage mutation
SARS-CoV-2 Pseudotyped virus with Spike(S1+S2) N501Y mutation mutation
SARS-CoV-2 Pseudotyped virus with Spike(S1+S2)-HV 69-70 Deletion mutation(ΔH69/ΔV70) mutation

GeneMedi’s Pseudovirus Based Neutralization Assay (PBNA) is a conventional assay method that is suitable for High-Throughout Screening (HTS) without live virus engaged. The Pseudovirus Based Neutralization Assay can be used for evaluating:

1) Neutralizing antibodies
2) Peptides blockers (peptide inhibitors)
3) Types of Vaccines3
4) Compounds targeting Spike induced cell-fusion.

GeneMedi offers:

1.SARS-CoV-2 Pseudotyped virus packaging and production
2.Effector cells: human ACE2 overexpression stable HEK293T cell lines
3.SARS-CoV-2(2019nCoV) Pseudotyped Virus Based Neutralization Assay service.





GENEMEDI
6th Floor, Buiding No.2, Kangxin Road 3377, Shanghai, China
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