Canine NT proBNP antibody and antigen (recombinant protein)

Diagnostic anti-Canine NT proBNP antibodies pairs and antigen for animal health (animal Dog/Canine Heart failure, cardiac disease) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.DescriptionUS $ Price (per mg)
GMP-CAN-NT-proBNP-Ag01Canine NT proBNP$3090.00
GMP-CAN-NT-proBNP-Ab01Anti-Canine NT proBNP mouse monoclonal antibody (mAb)$3090.00
GMP-CAN-NT-proBNP-Ab02Anti-Canine NT proBNP mouse monoclonal antibody (mAb)$3090.00
GMP-CAN-NT-proBNP-Ab03Anti-Canine NT proBNP human monoclonal antibody (mAb)$3090.00
GMP-CAN-NT-proBNP-Ab04Anti-Canine NT proBNP human monoclonal antibody (mAb)$3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-CAN-NT-proBNP-Ag01
Product NameCanine NT proBNP
Target/BiomarkerCanine NT proBNP
Expression platformE.coli
IsotypesRecombinant Antigen
Bioactivity validationAnti-Canine NT proBNP antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in NT proBNP level test of animal Dog/Canine with Heart failure, cardiac disease.
TagHis
Reconized/Reactive SpecicsCanine NT proBNP
Product descriptionRecombinant Canine NT proBNP protein was expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
PurityPurity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No.GMP-CAN-NT-proBNP-Ab01,GMP-CAN-NT-proBNP-Ab02
Target/BiomarkerCanine NT proBNP
Product NameAnti-Canine NT proBNP mouse monoclonal antibody (mAb)
Expression platformCHO
Isotypes Mouse IgG
Bioactivity validationRecombinant Canine NT proBNP antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Canine NT proBNP antibodies in Canine NT proBNP level test of animal Dog/Canine with Heart failure, cardiac disease.
Product descriptionAnti-Canine NT proBNP mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Canine NT proBNP antibodies.
PurityPurity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No.GMP-CAN-NT-proBNP-Ab03,GMP-CAN-NT-proBNP-Ab04
Target/BiomarkerCanine NT proBNP
Product NameAnti-Canine NT proBNP human monoclonal antibody (mAb)
Expression platformCHO
IsotypesHuman lgG1
Bioactivity validationRecombinant Canine NT proBNP antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Canine NT proBNP antibodies in Canine NT proBNP level test of animal Dog/Canine with Heart failure, cardiac disease.
Product descriptionAnti-Canine NT proBNP mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
PurityPurity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    1. Canine quantitative immunoassay reagent for NT-proBNPF

    We have created many monoclonal antibodies against canine NT-proBNP. All antibodies were examined as capture and detection antibodies in a sandwich immunoassay to find the best antibody pairing. 96-well plates are coated with capture antibodies, and europium chelates mark detection antibodies. To assess antibody pairings, two types of canine NT-proBNP were utilized as antigens: natural canine NT-proBNP from dog plasma and recombinant canine NT-proBNP (Cat. # GMP-CAN-NT-proBNP-Ag01). High sensitivity was demonstrated by many pairings in identifying native and recombinant NT-proBNP. Table 1 displays the best monoclonal antibody pairs.

    Capture antibody

    Detect antibodies

    GMP-CAN-NT-proBNP-Ab01

    GMP-CAN-NT-proBNP-Ab02

    GMP-CAN-NT-proBNP-Ab05

    GMP-CAN-NT-proBNP-Ab02

    GMP-CAN-NT-proBNP-Ab01

    GMP-CAN-NT-proBNP-Ab06

    Table 1. Most sensitive antibody pairs

    These immunoassays have a sensitivity of 22 pg/mL for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves.  Conversion of 1NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please note that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.

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    Figure 1, 2. NT-proBNP immunoassay calibration curve

    (A) Calibration curve for optimal paired detection

    (B) Calibration curve and canine plasma sample dilution comparison

    Reagent type: two-step sandwich immunofluorescence detection, streptavidin-coated microplate

    Capture antibody: 200ng/well, conjugated biotin

    Capture antibody: 200ng/well, labeled europium chelate

    Antigen: Canine recombinant NT-proBNP (Cat.#GMP-CAN-NT-proBNP-Ag01)

    Sample volume: 50μl

    Incubation time: 40 minutes at room temperature

    2. Quantitative detection of NT-proBNP in plasma of healthy dogs and dogs with heart disease

    These immunoassays have a sensitivity of 22 pg/ml 1 for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1 NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please be aware that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.

    Picture loading failed.

    Figure 3. NT-proBNP concentration in EDTA plasma samples from healthy dogs and dogs with heart disease.

    Reagent type: two-step sandwich immunofluorescence detection, streptavidin-coated microplate

    Capture antibody: GMP-CAN-NT-proBNP-Ab01, 1μg/well

    Capture antibody: GMP-CAN-NT-proBNP-Ab02, 200ng/well, labeled europium chelate

    Calibrator: Canine recombinant NT-proBNP (Cat.# GMP-CAN-NT-proBNP-Ag01)

    Sample volume: 50μl

    Incubation time: 40 minutes at room temperature

    3. Improved apparent stability of endogenous NT-proBNP in plasma samples

    The gradual breakdown of proteins over time poses a challenge in accurately measuring NT-proBNP content in samples (5-7). Proper sample handling and storage are crucial, but selecting antibodies for detection reagents is equally important. Immunoreactivity decreases if an epitope in either the capture or detection antibodies is broken or located at a protease cleavage point. Therefore, using antibodies that precisely target the stabilizing region can enhance analyte stability. In developing canine NT-proBNP detection reagents, it's vital to choose antibodies resistant to proteolytic degradation of NT-proBNP.

    Our antibodies were tested for their ability to detect endogenous canine NT-proBNP during sample storage. EDTA plasma from dogs with heart disease was incubated at two temperatures: +4°C and +20°C. At +4°C, NT-proBNP remains stable for at least 72 hours (95–105% of initial immunoreactivity, Figure 4A). Simultaneously, at +20°C, about 98% of the initial immunoreactivity is detectable after 24 hours (Figure 4B). Using our recommended antibody pairings, plasma can be stored at +4°C for at least 72 hours with minimal loss of immunoreactivity. However, at room temperature, there is a slight weakening of the signal during the first 24 hours. Note that individual plasma or serum samples may vary.

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    Figure 4 , 5. Stability of endogenous canine NT-proBNP in EDTA plasma

    (A) Immunoreactivity of NT-proBNP in plasma samples incubated for 24, 48 and 72 hours at +4°C.

    (B) Immunoreactivity of NT-proBNP in plasma samples incubated for 4, 8, 24 and 48 hours at +20°C. Collect protease inhibitor-free EDTA plasma; samples are centrifuged, separated, and stored at -70°C before use. EDTA plasma is stored at +4°C or +20°C with the addition of 0.1% NaN3 to prevent bacterial growth. After incubation, samples were stored at -70°C before measurement. The NT-proBNP concentration in plasma was 9 ng/ml (determined by GMP-CAN-NT-proBNP-Ab01 and GMP-CAN-NT-proBNP-Ab02 immunoassays). Detection using GMP-CAN-NT-proBNP-Ab01 and GMP-CAN-NT-proBNP-Ab05 as capture antibodies and GMP-CAN-NT-proBNP-Ab06 and GMP-CAN-NT-proBNP-Ab02 as detection antibodies respectively The system results are depicted in Figure 3.

    Click to get more Data / Case study about the product.



    Target/Biomarker Information


    N-terminal pro-B-type natriuretic peptide (NT-proBNP) in dogs is a fragment of the B-type natriuretic peptide (BNP) precursor molecule, primarily synthesized and released from cardiac myocytes in response to myocardial stretch and stress. This biomarker has emerged as a cornerstone in veterinary cardiology, providing critical insights into canine cardiac health.

    The production of NT-proBNP is intimately linked to cardiac function in dogs. Specifically, the ventricles of the heart, notably the left ventricle, serve as the primary source of NT-proBNP secretion. When these cardiac muscles encounter stress, strain, or injury, such as in cases of valvular heart disease, dilated cardiomyopathy, or congestive heart failure (CHF), NT-proBNP is released into the bloodstream.

    NT-proBNP serves as a sensitive and specific indicator of cardiac stress and dysfunction in dogs. Its measurement allows veterinarians to assess the presence and severity of various cardiac diseases, facilitating early diagnosis and tailored treatment strategies.



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