Infectious Bovine Rinotracheitis Virus glycoprotein gIII antibody and antigen (recombinant protein)

Diagnostic anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease Infectious Bovine Rinotracheitis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P067-Tg001-Ag01 Recombinant Infectious Bovine Rinotracheitis Virus glycoprotein gIII protein $3090.00
GMP-VT-P067-Tg001-Ab01 Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P067-Tg001-Ab02 Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P067-Tg001-Ab03 Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII human monoclonal antibody (mAb) $3090.00
GMP-VT-P067-Tg001-Ab04 Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII human monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P067-Tg001-Ag01
Product Name Recombinant Infectious Bovine Rinotracheitis Virus glycoprotein gIII protein
Pathogen Infectious Bovine Rinotracheitis Virus
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Infectious Bovine Rinotracheitis Virus level test of animal Bovines/Cattle infectious disease with Infectious Bovine Rinotracheitis.
Tag His
Product description Recombinant Infectious Bovine Rinotracheitis Virus glycoprotein gIII proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P067-Tg001-Ab01,GMP-VT-P067-Tg001-Ab02
Pathogen Infectious Bovine Rinotracheitis Virus
Product Name Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Infectious Bovine Rinotracheitis Virus glycoprotein gIII antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Bovine Rinotracheitis Virus antibodies in Infectious Bovine Rinotracheitis Virus level test of animal Bovines/Cattle infectious disease with Infectious Bovine Rinotracheitis.
Product description Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Bovine Rinotracheitis Virus antibodies.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P067-Tg001-Ab03,GMP-VT-P067-Tg001-Ab04
Pathogen Infectious Bovine Rinotracheitis Virus
Product Name Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII human monoclonal antibody (mAb)
Expression platform CHO
Isotypes Human lgG1
Bioactivity validation Recombinant Infectious Bovine Rinotracheitis Virus glycoprotein gIII antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Bovine Rinotracheitis Virus antibodies in Infectious Bovine Rinotracheitis Virus level test of animal Bovines/Cattle infectious disease with Infectious Bovine Rinotracheitis.
Product description Anti-Infectious Bovine Rinotracheitis Virus glycoprotein gIII mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


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    Pathogen Information


    Introduction:

    Infectious Bovine Rhinotracheitis Virus (IBRV), alternatively referred to as Bovine Herpesvirus 1 (BoHV-1), is a viral pathogen with significant implications for the cattle industry. As a member of the Herpesviridae family, this eukaryotic virus possesses a complex structure and a double-stranded DNA genome. It primarily infects cattle, triggering a multifaceted disease known as Infectious Bovine Rhinotracheitis (IBR). This article explores the classification, structure, hosts, associated diseases, and diagnostic methods related to IBRV.

    Pathogen Classification:

    IBRV belongs to the Herpesviridae family, placing it among eukaryotic viruses. Herpesviruses, characterized by their intricate structural composition, feature a lipid bilayer envelope surrounding an icosahedral capsid. Within this viral structure resides a double-stranded DNA genome, which differentiates them from prokaryotic pathogens like bacteria and phages.

    Pathogen Structure:

    IBRV boasts a complex structure encompassing various genes and proteins, each of which contributes to its pathogenicity. Among the essential viral proteins, glycoprotein D (gD) stands out. Glycoprotein D plays a pivotal role in the viral life cycle, particularly in mediating viral entry into host cells and facilitating cell-to-cell transmission.

    The IBRV genome contains a variety of other proteins that serve as virulence factors. These include glycoprotein B (gB), which participates in viral fusion and penetration, and glycoprotein E (gE), which is involved in cell-to-cell spread. Additionally, the virus's structural proteins play a role in the assembly and maturation of viral particles.

    Hosts and Associated Diseases:

    IBRV primarily infects cattle, with Bos taurus and Bos indicus being the main susceptible species. Once within the host, the virus can induce a spectrum of clinical manifestations:

    Infectious Bovine Rhinotracheitis (IBR): This is the hallmark disease caused by IBRV. It primarily manifests as an acute respiratory illness. Infected cattle may exhibit clinical signs such as nasal discharge, coughing, and increased respiratory rate. Severe cases can lead to pneumonia.

    Conjunctivitis: IBRV can also affect the eyes, leading to conjunctivitis, a condition characterized by inflammation of the conjunctival membranes. This can result in ocular discharge and discomfort.

    Vulvovaginitis: In female cattle, the virus can lead to pustular vulvovaginitis, causing inflammation and the formation of pustules in the reproductive tract.

    Abortion: Pregnant cows infected with IBRV may experience abortion, with the virus capable of crossing the placental barrier and inducing fetal death.

    Secondary Bacterial Infections: IBRV infection can weaken the immune system of affected cattle, rendering them susceptible to secondary bacterial infections that can exacerbate clinical signs.

    Diagnostic Methods:

    Accurate and timely diagnosis of IBRV is essential for disease management and control. Several diagnostic methods are employed, often targeting specific viral genes or proteins:

    Polymerase Chain Reaction (PCR): PCR amplifies specific segments of IBRV DNA, with commonly targeted genes including gD, gB, and gE. This method is highly sensitive and specific, allowing for the detection of viral DNA in various samples.

    Real-time PCR (qPCR): A more refined version of PCR, real-time PCR, is employed for quantitative measurement of viral DNA. It offers enhanced sensitivity and specificity and can target specific viral genes such as gD.

    Enzyme-Linked Immunosorbent Assay (ELISA): ELISA detects antibodies against IBRV proteins, such as gD and gB, in serum or plasma samples. This serological test is valuable for assessing the exposure and immune response of cattle to the virus.

    Virus Isolation: Culturing IBRV in cell cultures allows for the propagation of the virus and its subsequent identification, aiding in confirming the presence of the virus in clinical samples.

    Immunofluorescence Assay (IFA): IFA employs specific antibodies against IBRV proteins to identify the virus in tissue samples. It relies on the principle of binding fluorescent antibodies to viral antigens, making infected cells fluorescent under a microscope.

    Serological Tests: Various serological tests, including serum neutralization assays and hemagglutination inhibition tests, assess the presence of antibodies against IBRV proteins. These tests are crucial for monitoring the immune status of cattle herds.

    In summary, IBRV is a complex, eukaryotic virus responsible for causing Infectious Bovine Rhinotracheitis in cattle. Understanding its classification, structure, hosts, associated diseases, and diagnostic methods is vital for effective disease management and control within the cattle industry. Accurate and timely diagnosis, in particular, is crucial for implementing appropriate control measures and safeguarding cattle populations from the devastating effects of IBRV infection.



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