Pico Signal Western ECL Substrate

Pico Signal Western ECL Substrate is a hypersensitive ECL chemiluminescence kit produced by Genemedi for the detection of horseradish peroxidase (HRP) on immunoblots. It enables picogram- to high femtogram-level protein detection by western blot analysis, which can be visualized on X-ray film or an imaging system. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.

1. Picogram to femtogram sensitivity—detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane.

2. High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions.

3. Stable reagent—8-hour working solution stability; 1-year kit stability at room temperature.

4. Economical—optimized for dilute antibody concentrations:

· 2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock).

· 10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock).

5. Exceptional robustness—provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5K to 1:10K secondary antibody dilutions of a 1 mg/mL stock solution.

1. For more information please refer to Pico Signal Western ECL Substrate User Manual

1. What is the recommended dilution range for Hypersensitive ECL chemiluminescence Kit?

Answer

Primary antibody recommended dilution range is 0.2 to 1.0 µg/mL (1:1,000 to 1:5,000 dilution from a 1 mg/mL stock). Secondary antibody recommended dilution range is 10 to 50 ng/mL (1:20,000 to 1:100,000 dilution from 1 mg/mL stock).

2. What is the sensitivity of Hypersensitive ECL chemiluminescence Kit?

Answer

Hypersensitive ECL chemiluminescence Kit enables detection of low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane.