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Package Catalog No. Price(In USD) Qty (Quantity) Sum(In USD)
1mg GMP-V-2019nCoV-Smu-023-1mg 5990
10mg GMP-V-2019nCoV-Smu-023-10mg 38790
100mg GMP-V-2019nCoV-Smu-023-100mg 258000
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Description

Accession Number QHD43415.1
Expression platform 2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
IsotypesMamamlian (human cell)
TagC-His
Products descriptionRecombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD L452R mutant (double mutant variant B.1.617 lineage, S:E484Q+L452R (India)) was expressed in mammanlian cell (human cells) expression system with 6 HIS tag at the C-terminus.
Bioactivity validation ACE2 binding;
Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays;
PurityPurity: ≥95% (SDS-PAGE)
ApplicationSpike protein & ACE2 competition binding assay for efficacy evaluation of COVID-19 vaccines and therapeutic antibodies.
Immunogen in Elisa,lateral-flow tests,and other immunoassays;
Standard substance
The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass26kDa
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD L452R mutant (double mutant variant B.1.617 lineage, S:E484Q+L452R (India))

The world is in midst of the COVID-19 pandemic. Recently A new "double mutant" variant of the coronavirus has been detected from samples collected in India. The two mutations (E484Q and L452R mutations) come together in the same virus, may be more infectious or less affected by vaccines.1

Mutations of Spike protein in SARS-COV-2 (2019nCOV) double mutant variant B.1.617 lineage, S:E484Q+L452R (India)

Two of the spike mutations are at key residues in the RBD (E484Q and L452R), two are in the N-terminal domain (T95I and E154K).

Spike Mutation in SARS-COV-2 (2019nCOV) double mutant variant B.1.617 lineage, S:E484Q+L452R (India) Spike-S1 Subunit
N Terminal		 T95I
E154K Important residues mutation
Spike-RBD L452R Important residues mutation
E484Q Important residues mutation
Spike-S1 Subunit D614G A popular mutation in different new SARS-CoV-2 lineage
Spike-S2 Subunit P681R

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Figure. Amino acid changes in the spike region of the double mutant variant B.1.617 lineage, S:E484Q+L452R (India) genomes2