Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD (L452R+T478K) mutant (delta variant B.1.617.2 lineage

Order information

Package	Catalog No.	Price(In USD)	Qty (Quantity)	Sum(In USD)
1mg	GMP-V-2019nCoV-Smu-023-1mg	5990
10mg	GMP-V-2019nCoV-Smu-023-10mg	38790
100mg	GMP-V-2019nCoV-Smu-023-100mg	258000
≥100mg	GMP-V-2019nCoV-Smu-023-xmg	Inquiry
			Shipping Cost:	760.00
			Total:

Description

Accession Number	QHD43415.1
Expression platform	2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Isotypes	Mamamlian (human cell)
Tag	C-His
Products description	Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD L452R mutant (double mutant variant B.1.617 lineage, S:E484Q+L452R (India)) was expressed in mammanlian cell (human cells) expression system with 6 HIS tag at the C-terminus.
Bioactivity validation	ACE2 binding; Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays;
Purity	Purity: ≥95% (SDS-PAGE)
Application	Spike protein & ACE2 competition binding assay for efficacy evaluation of COVID-19 vaccines and therapeutic antibodies. Immunogen in Elisa,lateral-flow tests,and other immunoassays; Standard substance The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass	26kDa
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein RBD L452R mutant (double mutant variant B.1.617 lineage, S:E484Q+L452R (India))

The world is in midst of the COVID-19 pandemic. Recently A new "double mutant" variant of the coronavirus has been detected from samples collected in India. The two mutations (E484Q and L452R mutations) come together in the same virus, may be more infectious or less affected by vaccines.1

Mutations of Spike protein in SARS-COV-2 (2019nCOV) double mutant variant B.1.617 lineage, S:E484Q+L452R (India)
Two of the spike mutations are at key residues in the RBD (E484Q and L452R), two are in the N-terminal domain (T95I and E154K).
Figure. Amino acid changes in the spike region of the double mutant variant B.1.617 lineage, S:E484Q+L452R (India) genomes²